RESUMO
The aim of this research is investigation of angiotensin converting enzyme gene expression and angiotensin II activity in middle-aged men, following to eight weeks moderate exercise. This study was a semi-experimental research with a repeated measures design. From 96 volunteer middle-aged men, 20 subject [age range of 45-55 years] participated in this study after signing an informed consent form. Next, subject divided in two groups of training [10 person] and control [10] groups and performed the eight weeks moderate exercise training [time: 45 minutes, speed: 50-65 maximal heart rate, slope: 0%]. Blood samples were collected in three times: baseline, after 4 week and after 8 week, and Real time- PCR[Polymerase Chain Reaction] was used for evaluation of Angiotensin Converting Enzyme [ACE] mRNA and Elisa methods for angiotensin II [Ang II]. Angll activity in training group were not significantly changed after 4 weeks exercise training [p=0.255] but significantly increased after 8 weeks [p= 0.004]. In control groups Ang II increased after 4 and 8 weeks exercise training [respectively; p=0.952 and p=0.876]. ACE gene expression was significantly reduced in training group after 4 and 8 weeks [p=0.001], but in control group, ACE gene expression was not increased after 4 weeks of no regular exercises [p=0.35], but after 8 weeks, ACE mRNA significantly increased [p=0.001]. Moderate exercise training has reduced the genetic and blood markers of blood pressure in middle-aged men, but not doing regular exercises increased this factor