Cloning of 2.4 kb bovine herpesvirus-1 DNA fragment containing glycoprotein III gene into pUC18 plasmid vector.

A recombinant pBR322 plasmid containing bovine herpesvirus-1 HindIII 'I' fragment was analysed using EcoRI and BamHI restriction endonucleases. This recombinant plasmid was labelled with [alpha 32P]dATP and hybridized with southern blot of HindIII digested BHV-1 DNA fragments. A 2.4 kb double digested EcoRI-BamHI fragment of HindIII 'I' was subcloned into pUC18 plasmid to get complete gIII gene. The recombinant pUC18 plasmid was analysed for 2.4 kb BHV-1 DNA insert by restriction digestion with EcoRI and BamHI. Southern blot of restriction digested plasmid was hybridized with [alpha 32P]dATP labelled BHV-1 DNA probe.