RESUMO
A 26 year old male presented to the emergency department with alleged history of RTA and sustained run over injury to right lower limb. On examination his vitals were HR-132/min. BP-70/42 mmHg SpO2 – 97% with room air. He was papery pale, drowsy and arousable to calls. A provisional diagnosis of severe hypovolemic shock was made. 16G Venflon was secured and resuscitation initiated with crystalloids and colloids. Blood samples sent for routine investigations (CBC, RFT) and blood grouping, typing and coagulation parameters. Meanwhile during resuscitation patient had cardiac arrest with asystole for which CPR initiated and ROSC achieved in 5 minutes. His haemoglobin levels were 5 g% with ABG showing mild metabolic and lactic acidosis. He was then stabilised with 3 units of PRBC in ER and shifted to CT scans. OF CASE
RESUMO
Background : Human telomerase is a multi subunit ribonucleoprotein enzyme concerned with telomeric lengthening and homeostasis in man. This enzyme has been found to be elevated in inflammatory conditions like rheumatoid arthritis and silica injury lung. Since chronic periodontitis is also an inflammatory condition where immune cells and cytokines mediate tissue destruction, we set out to evaluate telomerase in gingival tissue samples from healthy subjects and chronic periodontitis patients by reverse transcriptase polymerase chain reaction. Materials and Methods : Gingival biopsies were obtained from eight healthy subjects and eight chronic periodontitis patients. Reverse transcriptase polymerase chain reaction (RTPCR) was carried out to evaluate telomerase gene expression in the samples. Results : None of the healthy gingival tissue samples expressed the telomerase gene while all the chronic periodontitis samples expressed it. The severe chronic periodontitis samples expressed the gene more intensely than the moderate chronic periodontitis samples. Conclusion : Various mechanisms have been explained to account for telomerase elevation in chronic periodontitis .This study helps us understand the role of telomerase in the pathogenesis of periodontal disease. It could be concluded that telomerase could be used as a marker to assess the severity of inflammation in chronic periodontitis.