RESUMO
Enteroinvasive Escherichia coli (EIEC) and Shigellaspp cause bacillary dysentery in humans by invading and multiplying within epithelial cells of the colonic mucosa. Although EIEC and Shigellashare many genetic and biochemical similarities, the illness caused by Shigellais more severe. Thus, genomic and structure-function molecular studies on the biological interactions of these invasive enterobacteria with eukaryotic cells have focused on Shigella rather than EIEC. Here we comparatively studied the interactions of EIEC and of Shigella flexneriwith cultured J774 macrophage-like cells. We evaluated several phenotypes: (i) bacterial escape from macrophages after phagocytosis, (ii) macrophage death induced by EIEC and S. flexneri, (iii) macrophage cytokine expression in response to infection and (iv) expression of plasmidial (pINV) virulence genes. The results showed thatS. flexneri caused macrophage killing earlier and more intensely than EIEC. Both pathogens induced significant macrophage production of TNF, IL-1 and IL-10 after 7 h of infection. Transcription levels of the gene invasion plasmid antigen-C were lower in EIEC than in S. flexneri throughout the course of the infection; this could explain the diminished virulence of EIEC compared to S. flexneri.
Assuntos
Humanos , Citocinas , Escherichia coli , Regulação Bacteriana da Expressão Gênica/imunologia , Macrófagos , Shigella flexneri , Fatores de Virulência/biossíntese , Morte Celular , ELISPOT , Escherichia coli , Genes Bacterianos , Regulação Bacteriana da Expressão Gênica , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Shigella flexneri , Fatores de VirulênciaRESUMO
The genetic relationship among the Escherichia coli pathotypes was investigated. We used random amplified polymorphic DNA (RAPD) data for constructing a dendrogram of 73 strains of diarrheagenic E. coli. A phylogenetic tree encompassing 15 serotypes from different pathotypes was constructed using multilocus sequence typing data. Phylogram clusters were used for validating RAPD data on the clonality of enteropathogenic E. coli (EPEC) O serogroup strains. Both analyses showed very similar topologies, characterized by the presence of two major groups: group A includes EPEC H6 and H34 strains and group B contains the other EPEC strains plus all serotypes belonging to atypical EPEC, enteroaggregative E. coli (EAEC) and enterohemorrhagic E. coli (EHEC). These results confirm the existence of two evolutionary divergent groups in EPEC: one is genetically and serologically very homogeneous whereas the other harbors EPEC and non-EPEC serotypes. The same situation was found for EAEC and EHEC.
Assuntos
Humanos , Técnicas de Tipagem Bacteriana , Escherichia coli/genética , Variação Genética , DNA Bacteriano/genética , Escherichia coli/classificação , Escherichia coli/patogenicidade , Fenótipo , Filogenia , Técnica de Amplificação ao Acaso de DNA Polimórfico , Análise de Sequência de DNA , Virulência/genéticaRESUMO
The genetic diversity of 41 typical and atypical enteropathogenic Ëscherichia coli" (EPEC) strains of the serogroup O55 was analysed by using the random amplified polymorphic DNA (RAPD) method. All typical EPEC O55 strains were grouped in two clusters (A and C) and belonged to serotype O55:H6, while cluster B included all atypical strains, which were of the serotype O55:H7. The three groups also included non-motile strains. RAPD may be a useful method for epidemiological studies on "E. coli" O55 infection