RESUMO
Objective To establish the effect of determining the content of notopterol and isoimperatorin in notopterygium from different habitat and harvest time by HPLC method .Methods HPLC instrument was used ,Waters symmetry C18(4 .6 mm × 250 mm ,5 μm) was adopted with a mobile phase of acetonitrile‐water (45∶55) ,the separation was carried out at a flow rate of 1 mL/min ,column temperature was 30 ℃ and the detection wavelength was 310 nm .Results Notopterol and isoimperatorin showed good linearity in the ranges of 0 .240 8-1 .505 0 μg and 0 .117 6-0 .735 0 μg with average recoveries rate (n=6) of 0 .98% and 1 .42%respectively .The content of notopterol and isoimperatorin in crude drugs of notopterygium from different habitat marked differ‐ence ,and harvest period and growth years made effect to content of two components .Conclusion HPLC method is easy and fast , and can be used as a basis for confirming the best habitat and harvest period of notopterygium .
RESUMO
Objective To study and establish the quantitative analysis method for verbascoside ,luteolin and apigenin in Siphono-stegia chinensis by HPLC .Methods HPLC was performed on Kromasil C18 (4 .6 mm × 250 mm ,5 μm) ,the mobile phase consisted of acetonitrile and water(containing 0 .1% phosphoric acid) at a flow rate of 1 .0 mL/min .The column temperature was set at 35℃ ,and the eluate was detected at 340 nm .Results Quantitative analysis of HPLC showed that the linear ranges of verbascoside , luteolin and apigenin were 0 .197 6~1 .482 μg (r=0 .999 8) ,0 .100 8~0 .756 μg (r=0 .999 7) ,0 .043 2~0 .324 μg (r=0 .999 7) respectively .The average recovery of verbascoside ,luteolin and apigenin were 99 .7% (RSD= 2 .0% ) ,99 .5% (RSD = 2 .3% ) , 99 .4% (RSD=2 .2% ) respectively .Conclusion The method is accurate ,feasible and reliable ,therefore it′s available for the quality control of Siphonostegia chinensis .