Correlation of Leptin and Vaspin Serum Concentration in Newly Diagnosed Type 2 Diabetes Mellitus

Objectives : The aim of the study was to explore the correlation of circulating leptin and vaspin levels with lipid profile, fasting blood sugar, HbA1c and anthropometric variable as inflammatory markers between diabetic patients and non - diabetic subjects. Material and Methods: This study was conducted with 120 newly diagnosed type 2 diabetes mellitus (T2DM) patients with age - matched 120 non - diabetic sub jects as controls. Results: We found that there is significant increase in the parameters like serum Leptin, Vaspin, FBS, PPBS, HbA1c and lipid profile (TC, TG & VLDL). No significant differences were found between BMI, LDL & HDL parameters of T2DM patient s compared to non - diabetic subjects. The results have been shown a significant positive correlation between Vaspin and Leptin in T2DM patients, (r = .755) and ( P ?0.01 ) as compared to controls. The body mass index was positively correlated with Vaspin in T2DM patients, (r = .50) and ( P <0.01 ) and with leptin in T2DM patients, (r = .265) and ( P <0.01) . A positive correlation had also observed between vaspin and LDL in T2DM patients, (r = .189) and ( p <0.05 ). We also found that significant increased leve l of leptin and vaspin in females compared to males in our study group. Conclusions: Serum leptin and vaspin level is positively associated with BMI and LDL and negatively correlated with fasting blood sugar, post - prandial glucose, HbA1c, VLDL and age.

Laboratory observations on the larvicidal efficacy of three plant species against mosquito vectors of malaria, Dengue/Dengue Hemorrhagic Fever (DF/DHF) and lymphatic filariasis in the semi-arid desert.

Comparative larvicidal efficacy of aqueous and organic solvent extracts from seeds, leaves and flowers of three desert plants viz. Calotropis procera (Aiton), Tephrosia purpurea (L.) Pers. and Prosopis juliflora (Sw.) DC. was evaluated against Anopheles stephensi (Liston), Aedes aegypti (Linnaeus) and Culex quinquefasciatus (Say). For this purpose larvae of all the three mosquito species were reared in the laboratory and studies carried out on late 3rd or early 4th instars using standard WHO technique. Based on concentration mortality data 24 and 48 hr LC50 and LC90 values along with their 95% fiducial limits, regression equation, chi-square (c2)/ heterogeneity of the response were determined by log probit regression analysis. Experiments were carried out with different solvent extracts of seeds of C. procera which revealed that methanol (24 hr LC50: 127.2, 194.8, 361.0) and acetone (229.9, 368.1, 193.0 mg l-1) extracts were more effective with the three mosquito species, respectively. Petroleum ether extract was effective only on An. stephensi while aqueous extracts were not effective at all with any of the mosquito species (mortality <10-30%). Tests carried out with methanol extracts of fresh leaves (24 hr LC50: 89.2, 171.2, 369.7) and flowers (24 hr LC50: 94.7,617.3, 1384.0 mg l-1) of Calotropis showed that preparations from fresh parts were 2-3 times more effective as compared to the stored plant parts. Efficacy was less than 10-30% with both An. aegypti and Cx. quinquefasciatus while An. stephensi was still susceptible to extracts from both leaves and flowers even after two years of storage. The 24 hr LC50 values as observed for methanol extracts of seeds of T. purpurea and leaves of P. juliflora were 74.9, 63.2 and 47.0 and 96.2, 128.1 and 118.8 mg l-1 for the above three mosquito species, respectively. Experiments carried out up to 500 mg l-1 with leaves (T. purpurea) and seeds (P. juliflora) extracts show only up to 10-30% mortality indicating that active larvicidal principle may be present only in the seeds of Tephrosia and leaves of Prosopis. In general, anophelines were found more susceptible than the culicines to the plant derived derivatives. More studies are being carried out on some other desert plants found in this arid region. The study would be of great importance while formulating vector control strategy based on alternative plant based insecticides in this semi-arid region.

Comparative larvicidal potential of different plant parts of Withania somnifera against vector mosquitoes in the semi-arid region of Rajasthan.

Larvicidal potential of the extracts from different parts viz. green and red fruits, seeds, fruit without seeds, leaves and roots of Withania somnifera in different solvents was evaluated against larvae of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus, the important disease vectors prevalent in the semi-arid region. Experiments were carried out on late 3rd or early 4th instar larvae of these mosquitoes using standard WHO technique. 24 and 48 hr LC 50 values along with their 95% confidence limits, regression equation, chi-square (c2)/ heterogeneity of the response have been determined by log probit regression analysis. The 24 hr LC50 values as observed for whole green fruits in water, methanol and petroleum ether were 350.9, 372.4, 576.9; 115.0, 197.1, 554.6; 154.9, 312.0, 1085.0 while corresponding values for red fruits were 473.5, 406.4, 445.2; 94.7, 94.5, 1013.0; 241.8, 535.0, 893.3 mg l-1 for An. stephensi, Ae. aegypti and Cx. quinquefasciatus respectively showing that methanol extracts were more effective against anophelines as compared to culicines when whole fruits were taken. The 24 hr LC50 values as observed for seeds in acetone, methanol and petroleum ether were 188.1, 777.5, 822.5; 245.5, 769.0, 1169.0; 140.3, 822.9, 778.4 and for fruit without seeds were 80.2, 97.6, 146.6; 88.4, 404.4, 1030.0; 30.0, 44.5, 54.2 mg l-1 for the above mosquito species respectively showing that extract of fruit without seeds were most effective in petroleum ether followed by acetone and methanol extracts. However, experiments conducted with methanol extracts of leaves and roots of this plant species did not show any appreciable larvicidal activity and a 20-40% mortality was observed up to 500 mg l-1 of the extracts. Overall larvae of anophelines were found more susceptible as compared to culicines to all the extracts tested. Petroleum ether extract of fruit without seeds was found most effective against all the mosquito species showing that active ingredient might be present in this part of the plant species. The study would be of great importance while planning vector control strategy based on alternative plant derived insecticides.

Evaluation of larvicidal efficacy of Solanum xanthocarpum storage against vector mosquitoes in north - western Rajasthan.

Efficacy of the methanol extracts from fruits without seeds, whole yellow ripe fruits and seeds of the plant Solanum xanthocarpum was evaluated against larvae of Anopheles culicifacies, Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus, the important vector mosquitoes prevalent in the arid region. Experiments were carried out on late 3rd or early 4th instar larvae of these mosquitoes using standard WHO technique. 24 and 48 hr LC50 and LC90 values along with their fiducial limits, regression equation, chi-square (x2)/ heterogeneity of the response have been determined by log probit regression analysis. The 24 hr LC50 values as observed for fruit without seeds, whole fruits and seeds after six months were 79.6, 91.7 and 131.7; 131.4, 186.9 and 195.6; 273.4, 290.9 and 377.6 and 384.9, 450.6 and 520.0 mgl-1 for An. culicifacies, An. stephensi, Ae. aegypti and Cx. quinquefasciatus respectively. However, the 24 hr LC50 values for fresh yellow ripe fruits and seeds determined previously were 51.6 and 66.9; 52.2 and 73.7; 118.3 and 123.8 and 157.1 and 154.9 mgl-1 for the above four vector species respectively showing that the efficacy was decreased two to three times more after six months of storage. The 24 hr LC50 values were also determined after one year of storage for fruit without seeds, whole fruits and seeds. The values were 103.3, 120.1 and 195.7; 146.3, 224.3 and 251.2; 316.9, 336.5 and 426.2 and 393.0, 500.3 and 656.7 mgl-1 as compared to the 24 hr LC50 values of 47.4,51.6 and 66.9; 52.3,52.2 and 73.7; 108.0,118.3 and 123.8 and 141.1, 157.1 and 154.9 mgl-1 of all the three fresh preparations for the above four vector species respectively showing that the efficacy was further decreased after one year of storage. Further the extracts should be used when they have been prepared from the fresh plant parts instead of the old stored parts. Larvae of anophelines were found more susceptible as compared to culicine. HPLC and IR studies showed that active component might be present in the fruits and seeds of this plant species. The study would be of great importance while planning vector control strategy based on alternative plant derived insecticides.

Larvicidal activity of the extracts from different parts of the plant Solanum xanthocarpum against important mosquito vectors in the arid region.

Larvicidal efficacy of the aqueous and methanol extracts from green unripe and yellow ripe fruits, seeds, leaves and roots of the plant Solanum xanthocarpum was evaluated against Anopheles culicifacies, Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus, the important mosquito vectors prevalent in the arid region. Studies were carried out on late 3rd or early 4th instar larvae of these species using standard WHO technique. Based on concentration mortality data LC50 and LC90 values along with their fiducial limits, regression equation, chisquare (l2)/ heterogeneity of the response have been determined by log probit regression analysis. The 24 hr LC50 values as observed for aqueous extracts for green unripe and yellow ripe fruits were 112.7, 498.2 and 846.3 mgl-1 and 104.7, 267.7 and 832.2 mg l-1 for Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus respectively. However, the 24 hr LC50 values for methanol extracts of yellow ripe fruits and seeds were 51.6, 52.2, 118.3 and 157.1 mg l-1 and 66.9, 73.7, 123.8 and 154.9 mg l-1 for the above four vector species respectively. The results showed that larvae of anophelines were much more susceptible as compared to culicines to both the aqueous and methanol extracts from fruits and seeds of the plant tested. The methanol extracts were found 2-5 times more effective as compared to the aqueous extracts. The chi-square values calculated during the analysis did not show any heterogeneity of the response. Experiments conducted with aqueous and methanol extracts from leaves and roots of this plant species didn’t show any larvicidal activity against any of the mosquito species tested. The study would be of great importance while formulating vector control strategy based on alternative plant based insecticides.

Relative susceptibility of some common mosquito vector larvae to synthetic insecticidal compounds in north-western Rajasthan.

Relative susceptibility of three important mosquito vector larvae viz., Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus, prevalent in the arid region was determined against four organophosphates (malathion, fenitrothion, fenthion, temephos) and three synthetic pyrethroid (alphamethrin, deltamethrin and fanvalerate) compounds. Studies were carried out on late 3rd or early 4th instar larvae of these species using standard WHO technique. Based on concentration mortality data LC50 and LC90 values along with their fiducial limits, regression equation, chi-square (chi2)/heterogeneity of the response have been determined by log probit regression analysis. LC50 values as observed for the above seven insecticides were 0.8097, 0.0398. 0.0432, 0.0035, 0.0025, 0.0092, 0.1006; 1.2370, 0.0531, 0.0655, 0.0076, 0.00004, 0.00004, 0.0046 and 1.4980, 0.0719, 0.0817, 0.0056, 0.00021, 0.00073, 0.0112 mg/l for the above three mosquito species respectively Among the four organophosphates tested temephos was the most effective followed by fenitrothion, fenthion and malathion. In general, Anopheles was found more susceptible as compared to the other two culicines to the above four compounds. The results also showed that larvae of Ae. aegypti were most susceptible followed by Cx. quinquefasciatus and An. stephensi to all the three pyrethroids tested. Among the three compounds tested alphamethrin was found to be the most toxic followed by deltamethrin while fanvalerate was the least toxic. The study would be of great importance while planning use of these insecticides for the control of different vector species in this area.

Study on serum and urinary cortisol levels of asthmatic patients after treatment with high dose inhaled beclomethasone dipropionate or budesonide.

BACKGROUND: The potential for long-term adverse effects from inhaled corticosteroids relates to their systemic absorption. With increasing use of high dose inhaled corticosteroids, there is need to establish whether similar doses of beclomethasone dipropionate (BDP) and budesonide (BUD) produce clinically important differences in untoward side effects specially hypothalamo-pituitary-adrenal (HPA) axis suppression. METHODS: Fifteen asthmatic patients were started on BDP or BUD (2000 microg/day) through spacer for six weeks. Serum cortisol (9 AM and 4 PM), 24-hour urinary steroid and pulmonary function testing parameters were performed. RESULTS: The serum cortisol levels were not found to be suppressed with either BDP or BUD. Similarly no significant changes were found in 24 hours urinary excretion of steroids with either of the drugs. Significant improvement was found in values of forced expiratory volume in the first second (FEV1) with BDP. With BUD the changes in forced vital capacity (FVC) and FEV1 were found to be significant. CONCLUSION: BDP or BUD in high doses of 2000 microg/day given upto six weeks through spacer are equally effective for treatment of bronchial asthma and do not cause any significant change in serum and urinary cortisol levels, and adrenal function/HPA axis.

Efficacy of different organophosphate and synthetic pyrethroid insecticides to the larvae of malaria vector Anopheles stephensi, Liston.

Evaluation of the potency of four organophosphate (OP) (Malathion, Fenthion, Fenitrothion and Temephos) and two synthetic pyrethroid (SP) compounds (cypermethrin and deltamethrin) was carried out against larvae of Anopheles stephensi, the major malaria vector in district Bikaner (Rajasthan). Different concentrations supplied by WHO were used for determining the percent mortalities. LC50 values as calculated by probit analysis were 1.2740, 0.0600, 0.0405, 0.0046, 0.0019 and 0.0016 mg/l respectively for the above six insecticides. Both SP compounds were found more toxic followed by temephos among the OP compounds. Malathion was found the least toxic while fenthion and fenitrothion were in between these two extremes.

Insecticide susceptibility status of Aedes aegypti to DDT and dieldrin in desert and non-desert parts of Rajasthan.

Seasonal prevalence and insecticide susceptibility tests were carried out on the adults of Aedes aegypti, the vector of dengue and dengue hemorrhagic fever(DF/DHF), in three desert (Bikaner, Jaisalmer and Jodhpur) and three non-desert (Alwar, Ajmer and Jaipur) districts of Rajasthan. Among the three species viz. Ae. aegypti, Ae. vittatus and Ae. w-albus encountered during the survey the former was the most prevalent species. Studies were carried out both in rural and urban areas against different concentrations of two organochlorines (DDT and Dieldrin). In rural areas resistance has been observed with DDT and dieldrin both in desert and non-desert parts while an intermediate resistance has been observed in the urban areas with both the insecticides which required further verification. Resistance was more pronounced in rural areas as compared to urban areas. LC50 along with regression equation and Chi-square values have been determined for both the insecticides.

Multiple isoforms of protein kinase C in lymphocytes and airway smooth muscle of guinea pig.

The isoenzyme pattern of protein kinase C (PKC) in lymphocytes and airway smooth muscles (ASM) was examined by Western blot using commercially available monoclonal antibodies. The results showed the presence of PKC alpha, beta, gamma, epsilon, eta, mu and zeta in lymphocytes and PKC alpha, gamma, epsilon, eta and zeta in ASM. The unexpected feature was the presence of PKCgamma in both lymphocytes and ASM of guinea pigs. Expression of this PKC isoform is usually restricted to tissues in the central nervous system or spinal cord. Expression of PKC delta, theta, lambda and tau was not detected in either lymphocytes or ASM.

Effect of feeding protein deficient diet on phospholipid turnover and protein kinase C mediated protein phosphorylation in rat brain.

Feeding of protein deficient diet is known to alter the transmembrane signalling in brain of rat by reducing total protein kinase C (PKC) activity. Phospholipid metabolism regulates the activation of PKC through generation of second messengers and the extent of PKC activation accordingly influences the magnitude of phosphorylation of its endogenous substrate proteins. Thus it was speculated that ingestion of protein deficient diet may modify the turnover rate of membrane phospholipids and magnitude of phosphorylation of endogenous substrate proteins of PKC. The experiments were conducted on rats fed on three different types of laboratory prepared diets viz. casein (20% casein), deficient (4% protein, rice flour as source of protein) and supplemented (deficient diet supplemented with L-lysine and DL-threonine) for 28 days. The metabolism of phosphoinositides (PIs) and phosphatidyl choline (PC) was studied by equilibrium labeling with [3H] myo inositol and [14C methyl] choline chloride respectively. The phosphorylation of endogenous substrate proteins of PKC was studied by using 32P-gamma-ATP followed by SDS-PAGE and autoradiography. The results suggest that in deficient group, there is an increased incorporation of [3H] myo inositol in PIs and inositol phosphate pool in comparison to the casein group. The phosphatidyl inositol (PI) turnover reduced, although there was a marginal increase in the phosphatidyl inositol monophosphate (PIP) and phosphatidyl inositol bis phosphate (PIP2). Supplementation of diet showed a reversal of the pattern towards control to a considerable extent. In the deficient group, PC metabolism showed an increased incorporation of [14C methyl] choline in choline phospholipids but decreased incorporation in phosphoryl choline in comparison with the casein group. The increase in total PC contents was significant but marginal in residue contents. The turnover rate of PC increased only marginally and that of residue declined. Supplementation of diet reduced the total contents of PC and residue, but the turnover rate of PC and residue remained still higher. Phosphorylation of endogenous proteins showed four different proteins of 78, 46, 33 and 16 kDa to be the substrates of PKC in casein group. In deficient group, phosphorylation of these proteins increased markedly while supplementation of diet had a reversing effect rendering the values to be intermediate between casein and the supplemented group. The changes in phospholipid metabolism and in phosphorylation of endogenous substrate proteins of PKC suggest that dietary protein deficiency causes alterations in transmembrane signalling mechanism in rat brain. These effects are partially reversed by improving the quality of proteins in the diet.

Effect of fiberoptic bronchoscopy on arterial blood gases and cardiac rhythm at a moderate altitude of 2250 meters.

OBJECTIVES: To study the effects of fiberoptic bronchoscopy (FOB) at an altitude of 2250 m on arterial blood gases (ABG) and cardiac rhythm abnormality. METHODS: Fifty consecutive patients undergoing fiberoptic bronchoscopy were evaluated for the arterial blood gases and cardiac rhythm changes at Shimla (a moderate altitude of 2250 m), where there is a state of ambient hypoxia. RESULTS: The changes were noted in five stages ranging from the levels before the procedure till 15 minutes after the completion of the procedure. The mean fall in PaO2 levels in this study was 8 +/- 2.45 mm Hg and the fall was maximum at the end of procedure. Both smokers and nonsmokers showed a significant fall but the fall was more severe in smokers. The mean fall in SaO2 in this study was 3%. The increase in heart rate and blood pressure during FOB was significant as compared to baseline levels. There was no significant change in PH, PaCO2, HCO3. The commonest rhythm abnormality noted was sinus tachycardia which was well tolerated. No major cardiac arrhythmia was noted. It was further seen that the duration of the procedure and type of special procedure undertaken did not effect the levels significantly. Cyanosis was the commonest complication encountered (36%) and was seen more frequently in smokers and those with age more than 40 years. It was observed during the induction of bronchoscope and also during the further negotiation of the bronchoscope into the smaller branches of bronchial tree. CONCLUSION: The changes in ABG and cardiac rhythm are comparable to the studies at sea level except the increased incidence of cyanosis.

Effectiveness of salmeterol in stable COPD.

Thirty patients of stable chronic obstructive pulmonary disease (COPD) were studied for evaluation of effectiveness of inhaled salmeterol in a double blind, randomised, placebo controlled trial for seven days. Baseline spirometery and breathlessness score after 6 minutes walk test were assessed in all patients. Bronchial asthma was excluded in each patient by reversibility test with 200 ug of salbutamol inhalation. After inhalation of two puffs from a given inhaler through spacer device in the morning, spirometery was carried out 1, 3, 6 and 12 hrs after inhalation on day 1, 3 and 7 of trial. Six minutes walk test and breathlessness score were assessed after 3 hrs of inhalation on days 1, 3 and 7. The maximum bronchodilator response to salmeterol was observed on 7th day at three hours after inhalation. The mean increase in FEV1 was 29.2% of baseline value, which was significant as compared to placebo where the mean increase in FEV1 was 3.3% of the baseline value. The peak rise in FEV1 was observed at three hours after inhalation on all the three days. The bronchodilator effect of salmeterol persisted for upto 12 hours. The mean maximum increase in walking distance in 6 minutes walk test was observed on 7th day in salmeterol group, which was 50.7 metres whereas in placebo group it was 12.2 meters. The patients in salmeterol group perceived less breathlessness after the six minutes walk test. The study concluded that salmeterol 50 micrograms twice a day is effective in improving lung functions in patients with stable COPD and can be recommended as maintenance therapy for stable COPD patients.

Dietary protein deficiency induced changes in protein kinase C activity and phospholipid metabolism in rat hepatocytes.

Dietary protein deficiency is known to alter the protein kinase C activity in various tissues of rats. Protein kinase C activity is influenced by the metabolism of membrane phosphoinositides and phosphatidyl choline (PC). For metabolic studies, hepatocytes have been the cells of choice of various workers. Therefore, studies on protein kinase C and these phospholipids were conducted in hepatocytes of rats maintained on three different diets viz. casein (20% protein) deficient (4% protein, rice flour as source of protein) and supplemented (deficient diet supplemented with L-lysine and DL-threonine) diet for 28 days. The protein deficiency in diet led to a decline in protein kinase C activity (P < 0.01) without effecting its translocation, an increase in phosphatidyl inositol 4,5-bisphosphate (P < 0.001) and a decrease in phosphatidyl inositol 4-monophosphate and phosphatidyl inositol (P < 0.01) but did not alter the PC contents, as compared to the casein group. Supplementation of deficient diet with L-lysine and DL-threonine could considerably reverse the effect of deficiency of protein in diet. The results suggest that quality of dietary protein is mainly relevant for maintaining phospholipid metabolism and physiology of hepatocytes and thus the signalling mechanism in these cells.

Current status of Anopheles stephensi response to various insecticides in some areas of the Thar desert.

Investigations on the current response of A. stephensi. to six insecticides viz. DDT, dieldrin, malathion, fenitrothion, propoxur and permethrin, were carried out in 3 districts i.e. Barmer, Jodhpur and Pali, of the Thar desert. The species was found resistant to DDT and dieldrin, partially resistant to malathion and susceptible to fenitrothion, propoxur and permethrin. Dieldrin and malathion resistance has been detected for the first time in the Thar desert. Lethal concentrations (LC50 & LC95) of DDT and dieldrin and lethal exposure times (LT50 & LT95) of malathion, fenitrothion, propoxur and permethrin have been determined. In some areas, the differences in LC50 and LT50 values of tested insecticides, except fenitrothion, were found statistically significant. Chi-square and regression tests have revealed the homogeniety and linear trend respectively in the response of A. stephensi to insecticides. The findings of the study indicate that organochlorine compounds can be used alternately in the spray operations.

Differential activity of protein kinase C in alveolar and peritoneal macrophages.

The characteristics of protein kinase C activity present in guinea pig alveolar and peritoneal macrophages have been compared and examined. The activity is predominantly cytosolic with preference for phosphatidyl serine as cofactor over other phospholipids. K(m) of protein kinase C for ATP is 30.30 and 54.05 microM in alveolar and peritoneal macrophages respectively. Scatchard plot analysis shows heterogenous binding sites for [3H]PDBu in alveolar macrophages in contrast to peritoneal macrophages showing homogeneous type of binding sites. PMA activates protein kinase C in a dose-dependent manner and shows downregulation at higher concentration in both alveolar and peritoneal macrophages. Endogenous proteins of molecular masses 77, 47, 37 and 16.5 kDa in alveolar macrophages and 77, 47, 38 and 15.5 kDa in pertioneal macrophages are phosphorylated by PKC. These findings suggest that alveolar and peritoneal macrophages possess two different types of protein kinase C activities but phosphorylate similar proteins and exhibit functional similarities in these cells.

Effect of dietary protein manipulation on translocation of protein kinase C activity in various tissues of rats.

Ingestion of protein deficient diet is known to decrease the enzyme load, particularly drug metabolising enzymes in liver. It also leads to decrease in polyphosphoinositide pool in brain and kidney. Therefore, changes in protein kinase C activity and its translocation were speculated and studied in brain, lung, heart, spleen, liver and kidney of rats maintained on three different diets, viz. casein (20% protein) deficient (4% protein, rice flour as protein source) and supplemented (deficient diet supplemented with L-lysine and DL-threonine), for 28 days. A significant alteration in total protein kinase C activity and/or its translocation was observed in these tissues in the deficient group in comparison to casein group. Supplementation of diet with L-lysine and DL-threonine could partially reverse the affect. These changes in protein kinase C activity and its translocation indicate alteration in the mechanism of signalling system in dietary protein deficiency and hence an altered response of tissues to the external stimuli in dietary protein deficiency.

Susceptibility status of Phlebotomus papatasi and Sergentomyia punjabaensis (Diptera:Psychodidae) to some insecticides in district Bikaner (Rajasthan).

Susceptibility tests were carried out on the females of two species of sandflies viz. Phlebotomus papatasi and Sergentomyia punjabaensis against six insecticides viz. DDT, dieldrin (organochlorines); malathion, fenitrothion (organophosphates); propoxur (carbamate) and permethrin (synthetic pyrethroid) in district Bikaner, Rajasthan. A concentration and time dependent effect was observed with insecticides for both the species. P. papatasi was found resistant to DDT, dieldrin and propoxur while susceptible to malathion, fenitrothion and permethrin. However, S. punjabensis was found susceptible to all the insecticides tested. LC50 and LT50 value estimated for DDT and dieldrin for P. papatasi and S. punjabaensis were found to be 2.2 and 0.3% x 1 hr and 1.45 and 0.032% x 15 min. respectively.