Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 28
Filtrar
1.
Chinese Journal of Hematology ; (12): 839-844, 2018.
Artigo em Chinês | WPRIM | ID: wpr-1011880

RESUMO

Objective: To elucidate the expression levels of key immune biomarkers, phosphate and tension homology deleted on chromosome ten (PTEN) and programmed cell death protein1(PD-1),of different immune tolerance pathway in classic Hodgkin's lymphoma (CHL) to further determine their clinical role and prognostic significance. Methods: The clinical features and prognostic factors of 56 CHL patients, who were admitted to the TianJin Medical University Cancer Institute from February 2003 to August 2013, were retrospectively analyzed. PTEN and PD-1 protein expression levels were analyzed by immunohistochemistry, Epstein-Barr virus encoded RNA (EBER) was performed by in situ hybridization assay. Correlations between the expression of biomarkers and clinicopathologic parameters were examined and survival analyses were performed. Results: This cohort of 56 CHL patients included 34 males and 22 females with a median age of 25 years (ranged from 7 to 71 years). In a univariate analysis, age≥45, IPS score >2, EBER positive, high expression of PTEN protein conferred inferior 5-year OS and 5-year PFS; In a multivariate model, age≥45, IPS score >2, EBER positive, high expression of PTEN protein were identified as the independent adverse prognostic factors for CHL. Conclusions: This study suggested for the first time that PTEN was independent prognostic immune biomarkers in CHL, which provided the novel therapeutic strategy of immune therapy for CHL.


Assuntos
Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Doença de Hodgkin , PTEN Fosfo-Hidrolase/análise , Prognóstico , Receptor de Morte Celular Programada 1/análise , Estudos Retrospectivos
2.
China Journal of Chinese Materia Medica ; (24): 957-962, 2015.
Artigo em Chinês | WPRIM | ID: wpr-330203

RESUMO

To study the protective effect of Arctigenin in goto-kakizaki (GK) rats combined with hypertension macroangiopathy. Six-week-old GK rats were divided randomly according to blood glucose level into four groups: the model group and low, middle and high dose arctigenin groups (12.5, 25, 50 mg x kg(-1)), with Wistar rats as the normal group. All of GK rats were given high-glucose and high-fat diet. After 16 weeks, GK rats were orally administrated with 10 mg x kg(-1) x d(-1) N-Ω-nitro-L-arginine methyl ester for eight weeks. During the modeling, all of arctigenin groups were orally administrated with different dose of arctigenin twice a day; The model group and the normal group were given solvents. At the beginning, mid-term and end of the experiment, blood glucose was measured. At the end of the experiment, efforts were made to detect blood pressure, collect abdominal aortic blood after anesthesia, fix thoracic aorta after bloodletting to make paraffin sections, observe morphological characteristics and detect the expression of VEGF by immunohistochemistry. According to the results, the blood glucose rose in all GK rats, with no significant difference between the drug group and the model group. At the end of the experiment, the blood pressure significantly increased in GK rats, indicating that Arctigenin could notably reduce the blood pressure in GK rats in a dose-dependent manner. The blood routine test showed increases in both the total white blood cell count and differential blood count, MPV and PDW, abnormal blood platelet parameters and decrease in PLT in GK rats, suggesting that Arctigenin could remarkably reduce the total white blood cell count and differential blood count, MPV and PDW. The thoracic aortic morphological observation revealed obvious endangium lesions in GK rats, demonstrating that Arctigenin could ameliorate the lesion extent. VEGF immumohistochemical staining showed a higher VEGF expression in the model group but lower expression in Arctigenin groups. In conclusion, Arctigenin had a protective effect on aorta in GK rats. Its mechanism may be related to blood pressure lowering, anti-inflammation, improvement in blood platelet function and reduction of VEGF expression.


Assuntos
Animais , Humanos , Masculino , Ratos , Glicemia , Metabolismo , Pressão Sanguínea , Diabetes Mellitus Tipo 2 , Metabolismo , Angiopatias Diabéticas , Metabolismo , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas , Furanos , Hipertensão , Metabolismo , Lignanas , Ratos Wistar
3.
Chinese Journal of Cancer ; (12): 28-40, 2015.
Artigo em Inglês | WPRIM | ID: wpr-349614

RESUMO

Metastasis is the main cause of cancer mortality. One of the initiating events of cancer metastasis of epithelial tumors is epithelial-to-mesenchymal transition (EMT), during which cells dedifferentiate from a relatively rigid cell structure/morphology to a flexible and changeable structure/morphology often associated with mesenchymal cells. The presence of EMT in human epithelial tumors is reflected by the increased expression of genes and levels of proteins that are preferentially present in mesenchymal cells. The combined presence of these genes forms the basis of mesenchymal gene signatures, which are the foundation for classifying a mesenchymal subtype of tumors. Indeed, tumor classification schemes that use clustering analysis of large genomic characterizations, like The Cancer Genome Atlas (TCGA), have defined mesenchymal subtype in a number of cancer types, such as high-grade serous ovarian cancer and glioblastoma. However, recent analyses have shown that gene expression-based classifications of mesenchymal subtypes often do not associate with poor survival. This "paradox" can be ameliorated using integrated analysis that combines multiple data types. We recently found that integrating mRNA and microRNA (miRNA) data revealed an integrated mesenchymal subtype that is consistently associated with poor survival in multiple cohorts of patients with serous ovarian cancer. This network consists of 8 major miRNAs and 214 mRNAs. Among the 8 miRNAs, 4 are known to be regulators of EMT. This review provides a summary of these 8 miRNAs, which were associated with the integrated mesenchymal subtype of serous ovarian cancer.


Assuntos
Feminino , Humanos , Cistadenocarcinoma Seroso , Genética , Patologia , Transição Epitelial-Mesenquimal , MicroRNAs , Fisiologia , Neoplasias Ovarianas , Genética , Patologia
4.
Chinese Journal of Cancer ; (12): 47-50, 2014.
Artigo em Inglês | WPRIM | ID: wpr-320536

RESUMO

In the research community, resistance to apoptosis is often considered a hallmark of cancer. However, pathologists who diagnose cancer via microscope often see the opposite. Indeed, increased apoptosis and mitosis are usually observed simultaneously in cancerous lesions. Studies have shown that increased apoptosis is associated with cancer aggressiveness and poor clinical outcome. Furthermore, overexpression of Bcl-2, an antiapoptotic protein, is linked with better survival of cancer patients. Conversely, Bax, CD95, Caspase-3, and other apoptosis-inducing proteins have been found to promote carcinogenesis. This notion of the role of apoptosis in cancer is not new; cancer cells were found to be short-lived 88 years ago. Given these observations, resistance to apoptosis should not be considered a hallmark of cancer.


Assuntos
Animais , Humanos , Apoptose , Fisiologia , Biomarcadores Tumorais , Metabolismo , Carcinogênese , Metabolismo , Caspase 3 , Metabolismo , Linfoma de Células B , Metabolismo , Patologia , Neoplasias , Metabolismo , Patologia , Proteínas Proto-Oncogênicas c-bcl-2 , Metabolismo , Resultado do Tratamento , Proteína X Associada a bcl-2 , Metabolismo , Receptor fas , Metabolismo
5.
Chinese Journal of Otorhinolaryngology Head and Neck Surgery ; (12): 400-405, 2012.
Artigo em Chinês | WPRIM | ID: wpr-316657

RESUMO

<p><b>OBJECTIVE</b>To investigate the contribution of vasculogenic mimicry (VM) and endothelium-dependent vessel (EDV) to invasion and metastasis of laryngeal squamous cell carcinoma (LSCC).</p><p><b>METHODS</b>A total of 203 cases with LSCC was reviewed and followed up. VM and EDV in LSCC tissues were assessed by double staining with anti-CD31 immunohistochemistry and periodic acid-schiff. Kruskal-Wallis test and one-way ANOVA were used to analyze the relationship between VM, EDV and clinical pathology parameters of LSCC. Kaplan-Meier analysis was used to evaluate overall survival (OS) of patients with LSCC.</p><p><b>RESULTS</b>VM related to pTNM stage, lymph node metastasis and pathology grade of LSCC, while EDV related to primary sites, pTNM stage, T stage and distant metastasis of LSCC. Univariate analysis showed VM (P = 0.014), pTNM stage (P = 0.009), T stage (P = 0.013), nodal status (P = 0.013), histopathology grade (P = 0.038), tumor size (P = 0.028), and radiotherapy (P < 0.0001) related to OS. VM (P = 0.011), primary sites (P = 0.049), tumor size (P = 0.001) and radiotherapy (P < 0.0001) related to disease free survival. Multivariate analysis indicated that VM was an adverse predictor for both OS and disease free survival.</p><p><b>CONCLUSIONS</b>Both VM and EDV existed in LSCC. VM contributed to progression of LSCC through promoting lymph node metastasis. VM is an independent predictor for the prognosis of LSCC.</p>


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma de Células Escamosas , Diagnóstico , Patologia , Endotélio Vascular , Neoplasias de Cabeça e Pescoço , Diagnóstico , Patologia , Neoplasias Laríngeas , Diagnóstico , Patologia , Metástase Linfática , Neovascularização Patológica , Prognóstico , Estudos Retrospectivos
6.
Chinese Medical Journal ; (24): 3445-3449, 2012.
Artigo em Inglês | WPRIM | ID: wpr-316490

RESUMO

<p><b>BACKGROUND</b>Recurrence and local lymph node metastasis affected the prognosis of patients with laryngeal cancer. The aim of this study was to analyze clinical and pathological significance of vasculogenic mimicry (VM) in laryngeal squamous cell carcinoma (LSCC) and evaluate its contribution to prognosis.</p><p><b>METHODS</b>Data of 168 cases of LSCC were reviewed retrospectively to reveal clinical pathology and prognostic significance of VM. CD31 and periodic acid-Schiff double staining was used to identify VM.</p><p><b>RESULTS</b>VM in LSCC contributed to lymph node metastasis (P = 0.003) and clinical progression. VM correlated to histopathology grade (P = 0.001) of LSCC. VM was an adverse prognostic factor for both disease-specific survival (P = 0.039) and metastasis-free survival (P = 0.042) by univariate survival analyses. And it was an independent prognostic factor for only disease-specific survival (P = 0.003) by multivariate survival analyses.</p><p><b>CONCLUSIONS</b>VM existed in LSCC. LSCC with VM has more potential to invasion and metastasis.</p>


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma de Células Escamosas , Patologia , Imuno-Histoquímica , Neoplasias Laríngeas , Patologia , Metástase Linfática , Patologia , Análise Multivariada , Prognóstico , Estudos Retrospectivos
7.
Chinese Journal of Pathology ; (12): 528-531, 2011.
Artigo em Chinês | WPRIM | ID: wpr-358306

RESUMO

<p><b>OBJECTIVE</b>To study the expression of MUM-1/IRF4 and its significance in follicular lymphoma.</p><p><b>METHODS</b>Ninety-eight cases of follicular lymphoma were enrolled into the study. They were graded according to the 2008 WHO criteria. The expression of MUM-1/IRF4 protein and other markers (CD10, bcl-6, bcl-2 and Ki-67) was studied using tissue microarray and immunohistochemistry.</p><p><b>RESULTS</b>Amongst the 98 cases studied, there were 24 grade 1 cases, 30 grade 2 cases, 26 grade 3A cases and 18 were grade 3B cases. The rates of expression of MUM-1/IRF4, CD10, bcl-6, bcl-2 and Ki-67 (≥ 25%) were 39.8% (39/98), 62.2% (61/98), 80.6% (79/98), 87.8% (86/98) and 50.0% (49/98), respectively. MUM-1/IRF4 predominantly expressed in high-grade follicular lymphoma and showed a significantly positive correlation with lymphoma grade (r = 0.628, P = 0.000) and Ki-67 index (r = 0.473, P = 0.000). MUM-1/IRF4 expression had a significantly negative correlation with CD10 expression (r = -0.597, P = 0.000), but no correlation with bcl-6 and bcl-2 expression.</p><p><b>CONCLUSIONS</b>MUM-1/IRF4 expression is significantly higher in high-grade follicular lymphoma, indicating that these cases have a high proliferative activity, more aggressive behavior and poorer prognosis. MUM-1/IRF4, when strongly expressed, is another helpful marker for the diagnosis of high-grade follicular lymphoma.</p>


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Biomarcadores Tumorais , Metabolismo , Proteínas de Ligação a DNA , Metabolismo , Fatores Reguladores de Interferon , Metabolismo , Antígeno Ki-67 , Metabolismo , Linfoma Folicular , Metabolismo , Patologia , Gradação de Tumores , Neprilisina , Metabolismo , Proteínas Proto-Oncogênicas c-bcl-2 , Metabolismo , Proteínas Proto-Oncogênicas c-bcl-6
8.
Chinese Medical Journal ; (24): 2883-2889, 2011.
Artigo em Inglês | WPRIM | ID: wpr-292784

RESUMO

<p><b>BACKGROUND</b>Recombinant human endostatin (rh-endostatin, Endostar) has been proved to be an inhibitor of angiogenesis. Docetaxel has been also considered as a common chemotherapeutic agent with inhibition of angiogenesis of malignancies. However, their function has been seldom compared and a best synergism protocol is not determined. This study aimed to compare the effects of two drugs, investigate their combined impact on human umbilical vein endothelial cells (HUVECs), a molecular basis and find ideal protocols to inhibit endothelial cell proliferation.</p><p><b>METHODS</b>HUVECs on confluent growth or activated by vascular endothelial growth factor (VEGF) were treated by rh-endostatin or/and docetaxel at respective gradient concentration in following operations as cell proliferation determined by MTT assay, cell cycle distribution, apoptosis and markers of CD146, CD62E and CD105 detected by flow cytometery, the structure of the channel formed by HUVECs measured by tube formation count.</p><p><b>RESULTS</b>Rh-endostatin exhibited time dependent inhibition of proliferation while docetaxel showed both time and dose dependent inhibition. HUVECs accumulated in G(0)-G(1) with decreased numbers of cells in G(2) after a single treatment of rh-endostatin or that followed by docetaxel treatment. Cells accumulated in G(2) after both a single docetaxel and simultaneous administration. Both the number of cells in G(0)-G(1) and apoptotic cells were increased by docetaxel followed by rh-endostatin treatment. The number of non-apoptotic cells at G(0)-G(1) was increased by first administering rh-endostatin then docetaxel. Sequential treatment of docetaxel followed by rh-endostatin resulted in the greatest increase in apoptosis (34.7%) and the second highest apoptosis was seen with simultaneous administration (18.2%). Expression of CD146 and CD105 on confluent HUVECs was reduced at certain doses of rh-endostatin and/or docetaxel. However, rh-endostatin reduced CD105 without any apparent impact on either CD146 or CD62E expression, whereas these markers were down-regulated by docetaxel after pre-activation by VEGF. Rh-endostatin treatment maintained tube-like structures for a limited time. In contrast, docetaxel swiftly reduced tube formation. Simultaneous treatment, or docetaxel followed by rh-endostatin, exhibited a stronger inhibition on tube formation than either agent alone.</p><p><b>CONCLUSIONS</b>Both rh-endostatin and docetaxel can inhibit HUVEC proliferation while the high apoptotic rate after combined administration was probably owing to different sequent administration by docetaxel followed by rh-endostatin or simultaneous treatment. Both proliferation and adhesion molecules on HUVECs of confluent growth are down-regulated by the two drugs. The rh-endostatin decreased proliferation markers, but only slightly modified adhesion molecules, while both markers were down-regulated by docetaxel on HUVECs activated by VEGF. Rh-endostatin could maintain adhesion of HUVECs at first then induce cells apoptosis to damage tube formation. We hypothesize that it could lead to vascular normalization in short time. In contrast, docetaxel can suppress HUVEC proliferation, adhesion, and reduced tube formation swiftly due to its cytotoxicity. Combined treatments can induce a synergistic inhibition of tube formation.</p>


Assuntos
Humanos , Antígenos CD , Metabolismo , Apoptose , Antígeno CD146 , Metabolismo , Proliferação de Células , Selectina E , Metabolismo , Endoglina , Endostatinas , Farmacologia , Citometria de Fluxo , Células Endoteliais da Veia Umbilical Humana , Biologia Celular , Receptores de Superfície Celular , Metabolismo , Proteínas Recombinantes , Farmacologia , Taxoides , Farmacologia
9.
Chinese Journal of Pathology ; (12): 332-337, 2010.
Artigo em Chinês | WPRIM | ID: wpr-333273

RESUMO

<p><b>OBJECTIVE</b>To study the expression of stomatin like protein-2 (SLP-2) at mRNA and protein levels in two kinds of malignant epithelial tumors, including laryngeal squamous cell carcinoma (LSCC) and invasive breast cancer, and to study the relations of SLP-2 expression and clinicopathologic parameters with the prognosis.</p><p><b>METHODS</b>RT-PCR and Western blot were used to detect the expression of SLP-2 mRNA and protein in LSCC and their normal counterparts (46 and 10 pair, respectively). Immunohistochemistry was carried on tissue array constructed from LSCC (104 cases) and breast cancer (263 cases), respectively. The association between SLP-2 expression and clinicopathologic parameters was analyzed.</p><p><b>RESULTS</b>LSCC showed a higher expression of SLP-2 than that of their normal counterparts (negative expression) at mRNA (83%, 38/46) and protein (7/10) level. Immunohistochemical analysis of LSCC showed that compared with negative expression in normal laryngeal epithelium (0/20), a higher SLP-2 expression was detected in LSCC (36/104, P=0.000) and associated with the advanced clinical stage (P<0.01) and lymph node metastasis (P=0.003). Immunohistochemical study of invasive breast cancer demonstrated that compared with negative expression in normal breast tissue (0/10), more than one half of the cases showed a high SLP-2 expression (52.5%, 138/263, P=0.000) in breast cancer, which correlated with the tumor size (P=0.020), lymph node metastasis (P<0.01), advanced clinical stage (P<0.01), distant metastasis (P=0.002) and HER2/neu protein expression (P=0.037). Survival analysis showed a shorter overall survival probability in patients with a high SLP-2 expression. It was considered that lymph node metastasis, positive HER2/neu expression, and high-level SLP-2 expression may act as the independent prognostic factors for those tumors.</p><p><b>CONCLUSIONS</b>A high expression level of SLP-2 may be associating with the development of invasion and metastasis in LSCC and breast cancer, and SLP-2 is also considered working as an independent factor indicating a poor prognosis clinically in breast cancer.</p>


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Proteínas Sanguíneas , Genética , Metabolismo , Neoplasias da Mama , Metabolismo , Patologia , Carcinoma Ductal de Mama , Metabolismo , Patologia , Carcinoma de Células Escamosas , Metabolismo , Patologia , Neoplasias Laríngeas , Metabolismo , Patologia , Metástase Linfática , Proteínas de Membrana , Genética , Metabolismo , Metástase Neoplásica , Recidiva Local de Neoplasia , Estadiamento de Neoplasias , Prognóstico , Modelos de Riscos Proporcionais , RNA Mensageiro , Metabolismo , Receptor ErbB-2 , Metabolismo , Análise de Sobrevida
10.
Chinese Journal of Oncology ; (12): 667-670, 2010.
Artigo em Chinês | WPRIM | ID: wpr-293530

RESUMO

<p><b>OBJECTIVE</b>To investigate gene mutations of epidermal growth factor receptor (EGFR) and K-ras in Chinese patients with non-small cell lung cancer (NSCLC) and its clinicopathological significance, and to analyze the correlation between these mutations and tumor response to erlotinib treatment.</p><p><b>METHODS</b>Mutations of exons 18, 19, 20 and 21 of the EGFR and codons 12, 13 of the K-ras in 301 cases of NSCLC were detected by PCR-amplification and gene sequencing. The relationship between the mutations and clinicopathological characteristics of the 301 patients was analyzed.</p><p><b>RESULTS</b>EGFR mutations were present in 32.9% (99/301) of the samples: 3 mutation in exon 18, 59 in exon 19, 2 in exon 20, and 35 in exon 21. Mutations of K-ras were present in 4.7% (14/301) of the samples: 13 in codon 12 and 1 in codon 13. EGFR mutations were never found in tumors with K-ras mutations, suggesting a mutually exclusive relationship. EGFR mutations were more common in adenocarcinomas, non-smokers and females. Seven out of 10 erlotinib-treated patients with disease control carried EGFR mutation.</p><p><b>CONCLUSION</b>The frequency of EGFR mutation in Chinese NSCLC patients is higher than that in Westerners, but the frequency of K-ras mutation is quite opposite. Combined detection of EGFR gene and K-ras gene mutation may help clinicians to choose patients who may gain benefit from EGFR tyrosine kinase inhibitor (EGFR-TKI) treatment, and to predict their response to erlotinib treatment and prognosis.</p>


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Adenocarcinoma , Tratamento Farmacológico , Genética , Patologia , Povo Asiático , Carcinoma Pulmonar de Células não Pequenas , Tratamento Farmacológico , Genética , Patologia , Carcinoma de Células Escamosas , Tratamento Farmacológico , Genética , Patologia , Códon , Cloridrato de Erlotinib , Éxons , Genes erbB-1 , Genes ras , Neoplasias Pulmonares , Tratamento Farmacológico , Genética , Patologia , Mutação , Inibidores de Proteínas Quinases , Usos Terapêuticos , Proteínas Proto-Oncogênicas , Genética , Proteínas Proto-Oncogênicas p21(ras) , Quinazolinas , Usos Terapêuticos , Receptores ErbB , Genética , Fatores Sexuais , Fumar , Proteínas ras , Genética
11.
Chinese Journal of Oncology ; (12): 767-770, 2010.
Artigo em Chinês | WPRIM | ID: wpr-293486

RESUMO

<p><b>OBJECTIVE</b>To investigate the mutation in mitochondrial DNA displacement-loop (mtDNA D-loop) region in oncocytoma and its relationship with tumorigenesis and tumor development.</p><p><b>METHODS</b>The mtDNA D-Loop region of 20 thyroid or renal oncocytomas and the adjacent normal tissues were amplified by PCR, and then sequenced. Five human fetal renal tissues were collected as matched controls.</p><p><b>RESULTS</b>Among the 20 oncocytomas, 21 mutations which focused on hypervariable region I (HVI) were found in 7 tumor tissues and 1 normal tissue with the mutation rates of 35% and 5%, respectively. At the same time, 191 polymorphisms were found in the 20 cases.</p><p><b>CONCLUSION</b>mtDNA D-loop region, especially HV I, is the mutational hotspot of oncocytomas, which may be closely related with mtDNA duplicating rate and the function of mitochondria.</p>


Assuntos
Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adenoma Oxífilo , Genética , Sequência de Bases , Análise Mutacional de DNA , DNA Mitocondrial , Genética , Neoplasias Renais , Genética , Mitocôndrias , Genética , Mutação , Polimorfismo Genético , Neoplasias da Glândula Tireoide , Genética
12.
Chinese Journal of Oncology ; (12): 830-833, 2010.
Artigo em Chinês | WPRIM | ID: wpr-293471

RESUMO

<p><b>OBJECTIVE</b>To study the expression of stomatin-like protein-2 (SLP-2) in esophageal squamous cell carcinoma (ESCC), and analyze the correlation between SLP-2 expression and clinicopathological features.</p><p><b>METHODS</b>The expression of SLP-2 protein in ESCC tissues (18 and 220 cases respectively) was detected by Western blot and IHC. The association between SLP-2 expression and clinicopathological features was analyzed.</p><p><b>RESULTS</b>Compared with normal epithelium, 13 cases of ESCC tissues showed a higher expression of SLP-2 on the protein level (72.2%, 13/18). IHC analysis on tissue microarray revealed that the expression rate of SLP-2 protein in ESCC was 54.1% and in normal esophageal mucosa was 3.6%, showing a significant difference (P < 0.001). SLP-2 high-level expression correlates with the extent of ESCC invasion (P = 0.033), but not with other clinicopathologic characteristics (P > 0.05).</p><p><b>CONCLUSION</b>SLP-2 as a novel cancer-related gene may play an important role in tumorigenesis of ESCC. The overexpression of SLP-2 may be closely associated with the invasion of esophageal cancer.</p>


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Proteínas Sanguíneas , Metabolismo , Fisiologia , Western Blotting , Carcinoma de Células Escamosas , Metabolismo , Patologia , Neoplasias Esofágicas , Metabolismo , Patologia , Imuno-Histoquímica , Metástase Linfática , Proteínas de Membrana , Metabolismo , Fisiologia , Invasividade Neoplásica
13.
Chinese Journal of Pathology ; (12): 402-407, 2009.
Artigo em Chinês | WPRIM | ID: wpr-249103

RESUMO

<p><b>OBJECTIVE</b>Bone-marrow derived mesenchymal stem cells (BMSC) have the potential to differentiate into endothelial cells. The aim of the study was to investigate the induction process of BMSC by B16 melanoma cells in vitro and to analyze the role of VEGF-a in the process.</p><p><b>METHODS</b>A co-culture system containing BMSC and B16 melanoma cells based on transwell indirect model was established, and the induction process of BMSC by B16 melanoma cells was studied in vitro.</p><p><b>RESULTS</b>BMSC were isolated from the bone marrow of C57 mice. BMSC expressed CD105, CD90, CD73, CD44 and CD166, and acquired expressin of endothelial phenotype markers including VEGFR-1, VEGFR-2 and Factor VIII after co-culture with B16 melanoma cells for 48 hours. The expression level of VEGFR-2 would be double and Factor VIII threefold more by extending the co-culture time to 72 hours. In the co-culture system, B16 melanoma cells also up-regulated the expression of VEGF-a.</p><p><b>CONCLUSIONS</b>VEGF-a plays a significant role in the differentiation of BMSC into cells of endothelial phenotype, therefore, is important to tumor angiogenesis.</p>


Assuntos
Animais , Masculino , Camundongos , Células da Medula Óssea , Biologia Celular , Metabolismo , Diferenciação Celular , Linhagem Celular Tumoral , Técnicas de Cocultura , Células Endoteliais , Biologia Celular , Metabolismo , Fator VIII , Metabolismo , Melanoma Experimental , Metabolismo , Patologia , Células-Tronco Mesenquimais , Biologia Celular , Metabolismo , Camundongos Endogâmicos C57BL , Projetos Piloto , Fator A de Crescimento do Endotélio Vascular , Metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular , Metabolismo , Receptor 2 de Fatores de Crescimento do Endotélio Vascular , Metabolismo
14.
Chinese Journal of Pathology ; (12): 585-589, 2009.
Artigo em Chinês | WPRIM | ID: wpr-249034

RESUMO

<p><b>OBJECTIVE</b>To explore the existence of vasculogenic mimicry (VM) in ovarian carcinoma and its correlationship with the clinicopathologic features and prognosis of the tumor.</p><p><b>METHODS</b>A total of 84 ovarian carcinoma cases were collected with complete clinical and prognostic data. CD31 immunohistochemistry and PAS special stain were used to investigate VM in the tumor tissue. Immunohistochemical staining of VEGF, MMP-2, MMP-9, E-cadherin, beta-catenin, and Vimentin were used to explore the pathogenesis of VM.</p><p><b>RESULTS</b>Totally 36 of 84 cases exhibited evidence of VM. FIGO classification, pathologic grades and histological types were significantly different between the VM and non-VM groups. Expression of VEGF, MMP-2, MMP-9, E-cadherin and beta-catenin were higher in the VM group than in the non-VM group. Kaplan-Meier survival curve analysis showed that cases of the VM group had a lower survival rate than that of the non-VM group (P = 0.04).</p><p><b>CONCLUSIONS</b>Vasculogenic mimicry exists in ovarian carcinoma. Ovarian carcinomas with a high grade malignancy have a high incidence of VM formation, a higher incidence of metastases and a lower survival rate. High expression of MMP-2 and MMP-9 may contribute to the formation of VM in the ovarian cancer.</p>


Assuntos
Feminino , Humanos , Pessoa de Meia-Idade , Caderinas , Metabolismo , Carcinoma Endometrioide , Metabolismo , Patologia , Cistadenocarcinoma Mucinoso , Metabolismo , Patologia , Cistadenocarcinoma Seroso , Metabolismo , Patologia , Metaloproteinase 2 da Matriz , Metabolismo , Metaloproteinase 9 da Matriz , Metabolismo , Metástase Neoplásica , Neovascularização Patológica , Metabolismo , Patologia , Neoplasias Ovarianas , Metabolismo , Patologia , Taxa de Sobrevida , Fator A de Crescimento do Endotélio Vascular , Metabolismo , beta Catenina , Metabolismo
15.
Chinese Journal of Otorhinolaryngology Head and Neck Surgery ; (12): 480-485, 2009.
Artigo em Chinês | WPRIM | ID: wpr-245899

RESUMO

<p><b>OBJECTIVE</b>Analyze the relationship between the expression of hypoxia-inducible factor-1alpha (HIF-1alpha) and epidermal growth factor receptor (EGFR) and CD105 micro vessel density (MVD) and their value in evaluating biologic behavior and prognosis in laryngeal cancer.</p><p><b>METHODS</b>Ninety-one cases of laryngeal cancer were analyzed about their clinical and pathology data. In tumor tissue the expression of HIF-1alpha and EGFR was detected by immunohistochemistry and MVD was marked by CD105.</p><p><b>RESULTS</b>The expression of HIF-1alpha was correlated with size, TNM stage, T stage, lymph node metastasis and histological grade (all P<0.05). The expression of EGFR was correlated with TNM stage, lymph node metastasis, histological grade and relapse (all P<0.05). MVD was correlated with type, TNM stage, T stage, lymph node metastasis, metastasis and histological grade (all P<0.05). The expression of HIF-1alpha and EGFR was correlated with MVD (F value was 7.644 and 5.197 respectively, P value was 0.001 and 0.025 respectively). The correlation between the expression of HIF-1alpha and EGFR was significant statistically (r= 0.238, P=0.007). The survival rate of patients of 3 years and 5 years were 56.1% and 44.2% respectively. Survival analysis by Log Rank showed that prognosis of laryngeal cancer patients was correlated with type, TNM stage and the expression of both HIF-1alpha and EGFR. While Cox multiple factors analysis demonstrated that TNM stage and expression of EGFR were independent prognostic factor of laryngeal cancer (P value was 0.049 and 0.041 respectively, RR was 1.300 and 2.417 respectively).</p><p><b>CONCLUSIONS</b>HIF-1alpha and EGFR are key molecular event during development and progression of laryngeal cancer, which act in regulating tumor angiogenesis as well, and show intimate relationship with biological behavior and prognosis of laryngeal cancer.</p>


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Vasos Sanguíneos , Patologia , Carcinoma de Células Escamosas , Metabolismo , Patologia , Subunidade alfa do Fator 1 Induzível por Hipóxia , Metabolismo , Neoplasias Laríngeas , Metabolismo , Patologia , Estadiamento de Neoplasias , Neovascularização Patológica , Prognóstico , Receptores ErbB , Metabolismo , Estudos Retrospectivos
16.
Chinese Journal of Pathology ; (12): 660-664, 2008.
Artigo em Chinês | WPRIM | ID: wpr-315118

RESUMO

<p><b>OBJECTIVE</b>To establish a method of SYT-SSX fusion gene detection by FISH and to explore its diagnostic value for synovial sarcoma.</p><p><b>METHODS</b>The presence of SYT-SSX fusion gene was determined by FISH using a tissue microarray containing 62 known synovial sarcomas, 60 non-synovial sarcomas and 133 equivocal synovial sarcomas. FISH results were compared with those of RT-PCR published previously.</p><p><b>RESULTS</b>Overall, 96.9% (247/255) of the cases were successfully analyzed by FISH. The sensitivity of FISH for known synovial sarcomas was 96.7% (58/60), and the specificity for the non-synovial sarcoma was 100% (59/59). Moreover, SYT-SSX gene fusion was detected in 78.1% (100/128) of the equivocal synovial sarcomas. The concordance rate between FISH and RT-PCR was 83.6% (102/122) in those equivocal synovial sarcomas, and overall 79.7% (106/133) of these cases were confirmed as synovial sarcomas either by RT-PCR or by FISH.</p><p><b>CONCLUSIONS</b>The sensitivity and specificity of FISH detection of SYT-SSX fusion gene are high. FISH and RT-PCR are complementary to each other in the confirmation of synovial sarcomas, particularly those questionable cases.</p>


Assuntos
Humanos , Biomarcadores Tumorais , Genética , Hibridização in Situ Fluorescente , Métodos , Hibridização de Ácido Nucleico , Métodos , Proteínas de Fusão Oncogênica , Genética , Patologia Molecular , Métodos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sarcoma Sinovial , Diagnóstico , Genética , Neoplasias de Tecidos Moles , Diagnóstico , Genética
17.
Chinese Journal of Pathology ; (12): 832-837, 2007.
Artigo em Chinês | WPRIM | ID: wpr-350001

RESUMO

<p><b>OBJECTIVE</b>To investigate the influence of different microenvironments on tumor microcirculation patterns and invasive capability.</p><p><b>METHODS</b>Melanoma B16 cells were injected into the peritoneal cavity and skeletal muscle of C57 mice synchronously. CK18 expression in melanoma was assessed to distinguish the malignant phenotype of tumors in the peritoneal cavity from that in the skeletal muscle. HIF-1alpha, MMP-2 and MMP-9 protein and mRNA expression were compared in the two microenvironments. Cells positive for each immunohistochemical stain and the vessels representative of each type of microcirculation pattern were evaluated in two microenvironments.</p><p><b>RESULTS</b>CK18 and HIF-1alpha expression in melanoma were significantly higher in the skeletal muscle than in the peritoneal cavity (t = 8.142, t = 3.645, P < 0.05). Compared with the peritoneal cavity, melanoma cells in the skeletal muscle overexpressed MMP-2 and MMP-9 (t = 4.916, t = 7.782, P < 0.05). Real time-PCR results also showed that MMP-2 and MMP-9 mRNA levels in melanoma were higher in the skeletal muscle than in the peritoneal cavity (t = 36.814, t = 26.025, P < 0.05). Vasculogenic mimicry channels and endothelium-dependent vessels were the major microcirculation patterns in the skeletal muscle and in the peritoneal cavity respectively.</p><p><b>CONCLUSIONS</b>Different microenvironments affect invasiveness and blood supply patterns of melanoma. Different microenvironment induced tumor cell secretion of more invasion-related proteins and affect invasiveness and blood supply patterns of melanoma.</p>


Assuntos
Animais , Camundongos , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica , Subunidade alfa do Fator 1 Induzível por Hipóxia , Genética , Metaloproteinase 2 da Matriz , Genética , Metaloproteinase 9 da Matriz , Genética , Melanoma , Genética , Metabolismo , Patologia , Camundongos Endogâmicos C57BL , Microcirculação , Músculo Esquelético , Metabolismo , Patologia , Invasividade Neoplásica , Cavidade Peritoneal , Patologia , Reação em Cadeia da Polimerase , RNA Mensageiro , Genética , Metabolismo
18.
Chinese Journal of Pathology ; (12): 480-484, 2007.
Artigo em Chinês | WPRIM | ID: wpr-347754

RESUMO

<p><b>OBJECTIVE</b>To assess the diagnostic values of immunohistochemistry and SYT-SSX fusion gene detection for synovial sarcoma.</p><p><b>METHODS</b>Based on clinical features, histological and immunohistochemical profiles, 195 cases of tumors were divided into three diagnostic categories: definitive synovial sarcoma, probable synovial sarcoma and possible synovial sarcoma. RT-PCR Detection of the SYT-SSX fusion gene was performed using paraffin embedded tissue samples. Comparison between RT-PCR and immunohistochemistry results was carried out and their diagnostic value was evaluated.</p><p><b>RESULTS</b>There were 62 (31.8%) definite synovial sarcomas, 49 (25.1%) probable synovial sarcomas and 84 cases (43.1%) possible synovial sarcomas. SYT-SSX fusion gene was detected in 140 (78.2%) cases overall, including 94.7% (54/57) definite synovial sarcomas, 86.0% (37/43) probable synovial sarcomas and 62.0% (49/79) possible synovial sarcomas. In tumors in the certain and probable synovial sarcoma categories, the positive rates of epithelial membrane antigen (EMA) were significantly higher in the SYT-SSX positive cases than SYT-SSX-negative cases (P = 0.022, P = 0.010, respectively). EMA was positively correlated with the presence of SYT-SSX (r(s) = 0.431, P = 0.001, r(s) = 0.463, P = 0.002, respectively). However, such a correlation was not seen in cytokeratin (CK), vimentin or S-100 protein immunostains (P > 0.05). In tumors of possible synovial sarcoma category, there were no significant differences of CK, EMA, vimentin or S-100 protein between SYT-SSX-positive and SYT-SSX-negative tumors.</p><p><b>CONCLUSIONS</b>SYT-SSX fusion gene detection is not needed when the conventional approaches are diagnostic. EMA positivity has a similar diagnostic value to that of SYT-SSX by RT-PCR for tumors in the probable synovial sarcoma category. However, detection of SYT-SSX is very important for diagnosis of the tumors in the category of possible synovial sarcoma.</p>


Assuntos
Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Biomarcadores Tumorais , Metabolismo , Imuno-Histoquímica , Queratinas , Metabolismo , Mucina-1 , Metabolismo , Proteínas de Fusão Oncogênica , Metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Proteínas S100 , Metabolismo , Sarcoma Sinovial , Diagnóstico , Metabolismo , Patologia , Neoplasias de Tecidos Moles , Diagnóstico , Metabolismo , Patologia , Vimentina , Metabolismo
19.
Chinese Journal of Oncology ; (12): 500-504, 2007.
Artigo em Chinês | WPRIM | ID: wpr-298565

RESUMO

<p><b>OBJECTIVE</b>To investigate the effects of endostatin and doxycycline on microcirculation patterns in melanoma and their molecular mechanisms.</p><p><b>METHODS</b>To establish mouse B16 melanoma model by subcutaneous injection of B16 melanoma cell suspension. The mice were divided into 3 experimental groups and 1 control group. To treat the mice in the 3 experimental groups with endostatin, doxycycline, endostatin and doxycycline, respectively, and the control group without any treatment. The tumor volume was measured and recorded to make comparison of their growth rate. To assess the expression of MMP-2, MMP-9 and TIMP-2 by immunohistochemical staining. The three microcirculation patterns of endothelium-dependent vessels, mosaic vessels and vasculogenic mimicry were counted. The activity of MMP-2, MMP-9 between different groups was examined by gelatin zymography.</p><p><b>RESULTS</b>Tumor growth in the three experimental groups was statistically significantly slower than that in the control group. The expression of MMP-2, MMP-9 and TIMP-2 in each treated group was significantly different with that in the control group. The amount of three microcirculation patterns in three experimental groups was less than that of the control group, and the amount of MV and VM in each experimental group was significantly less than that in the control group. By gelatin zymography, the enzyme activity of MMP-9, actived-MMP-2 and MMP-2/proMMP-2 in ES, DOX and ES + DOX group was lower than that in the control group, but the enzyme activity of pro-MMP-2 among the four groups was not significantly different.</p><p><b>CONCLUSION</b>The combined use of doxycycline and endostatin in melanoma can inhibit the expression of MMPs, influencing the formation of different microcirculation patterns in melanoma.</p>


Assuntos
Animais , Feminino , Masculino , Camundongos , Antineoplásicos , Farmacologia , Linhagem Celular Tumoral , Doxiciclina , Farmacologia , Combinação de Medicamentos , Sinergismo Farmacológico , Endostatinas , Farmacologia , Metaloproteinase 2 da Matriz , Metabolismo , Metaloproteinase 9 da Matriz , Metabolismo , Melanoma Experimental , Patologia , Camundongos Endogâmicos C57BL , Microcirculação , Microvasos , Patologia , Transplante de Neoplasias , Inibidor Tecidual de Metaloproteinase-2 , Metabolismo , Carga Tumoral
20.
Chinese Journal of Otorhinolaryngology Head and Neck Surgery ; (12): 419-424, 2006.
Artigo em Chinês | WPRIM | ID: wpr-298862

RESUMO

<p><b>OBJECTIVE</b>Identify the significance of variants in papillary thyroid carcinoma (PTC), the role of extracellular matrix (ECM), MMPs, cell adhesion molecular (CAM) and cytokine in lymphatic metastasis in PTC and the value of PET-CT in diagnosis of thyroid carcinoma.</p><p><b>METHODS</b>Five hundred and five cases of PTC which had complete medical records and followed up surveys were selected from the files. Clinical biological characteristic of the histological variants was investigated. Sixty cases of PTC were selected. As the important parts of micro ecosystems, ECM, MMPs, CAMs and cytokine were investigated in use of tissue chip and method of immunohistochemistry. In addition, a group of cases of thyroid carcinoma including PTC was analyzed, follicular thyroid carcinoma and medulla thyroid carcinoma (MTC) and their reports of PET-CT as the initiation.</p><p><b>RESULTS</b>The variants could be divided into three groups in terms of the rate of cervical lymph node metastasis. The high-metastases group included diffuse sclerosing variant, tall cell variant, column cell variant and diffuse follicular variant. The low-metastases group included macrofollicular variant and papillary microcarcinomas. Others are included in the moderate-metastases group. The rate of cervical lymph node metastasis of each group were 83.0% , 55.5% and 34.1% (P < 0. 05), respectively. The 10-year-survival were 75.3% , 95.8% and 100.0% , respectively. The 20-year-survival were 31.2%, 80.3% and 87.5%, respectively. The positive rate of LN, FN, MMP-2, MMP-9, TIMP-2, CD, Integrinbeta-1, ICAM-1, EGFR, TGFR-beta, VEGF-C and E-Cad in metastasis ranged from 51.6% (Integrinbeta-1) to 98.3% (CD), and that in primartumor ranged from 46.7% (FN) to 98.3% (ICAM-1). The expression of E-cad in primartumor was lower than that in normal tissues (P < 0.05). The sensitivity of PET-CT was 100% and the specificity was 85.7%.</p><p><b>CONCLUSIONS</b>The characteristic has significant difference among the variants. Individual treatment should be performed in terms of different variants. Furthermore, some molecules play an important role in the lymph node metastasis of PTC and may be considered as the focus of future study. In addition, compared with CT and Bus, PET-CT is more sensitive and has its unquestionable advantage as a whole body examination, especially for staging and detecting micro metastases, though it requires a high expense.</p>


Assuntos
Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Carcinoma Papilar , Diagnóstico por Imagem , Patologia , Metástase Linfática , Tomografia por Emissão de Pósitrons , Sensibilidade e Especificidade , Neoplasias da Glândula Tireoide , Diagnóstico por Imagem , Patologia , Tomografia Computadorizada por Raios X
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA