RESUMO
AIM: To explore the kinetics and thermodynamics parameters in the extraction of flavonoids from Fagopyrum tartarjcum . METHODS: Rutin was adopted as marker substance to create a regression equation between Rutin concentration and absorbance by meaus of UV. RESULTS: According to Fick diffusion principle Ⅱ, extraction process was analysed to gain parameters, such as K, Ea, Y, t 1/2 , K ?. CONCLUSION: The method would be useful for the process design and the choice of operation condition to extract flavonoids from Fagopyrum tartarjcum.
RESUMO
The effects of manganese on selenium content and glutathione peroxidaseactivity in blood and myocardium, and on selenium excretion in growing ratswere studied to ferret out the interaction of the two essential trace elements.Manganese dichloride (40mg/kg of MnCl2.4H2O) was administered daily to a group of 17 rats for 35 days intraperitoneally. An obvious increase in manganese content in serum and myocardium was induced. However, selenium levels in the two tissues of manganese-treated rats were significantly lower than the controls. From the 14th day on, glutathione peroxidase activities in whole blood of manganese-treated rats were below the controls, and on the 35th day, the activities of the selenium-containing enzyme in blood and myocardium were reduced by 29.33% (P
RESUMO
Albino rats fed on crops from endemic and non-endemic districts of Ke-shan desease, were used in this experiment, which was studied by 85Zn tracer metabolic method after 8 weeks feeding. Uptake of 85Zn (ZnCl2) by blood, heart, liver, kidneys and brown adipose tissue were determined at 24 and 72 hours after injection of this tracer respectively, and its distribution was thoroughly examined. The results revealed that the uptake of 8aZn was higher in the groups fed on crops from endemic districts, particularly significant in liver, kidneys and brown adipose tissue. This may reflect the lacking or deficiency of zinc in the bodies.The regular differences of 85Zn between two groups were certainly related to the crops produced from endemic or aon-endemic districts. In other words, there should be some differences between crops from endemic and non-endemic areas. Thus more attention should be paid to the factors in endemic crops, which induced lacking or deficiency of zinc in the experimental animal and to its relationship with etiology of Keshan disease.
RESUMO
Observations were conducted on 11 kinds of tissues and organs(brown adipose tissue, thymus gland, blood, heart, lungs, muscle, bone, kidneys, spleen, liver, and intestine) in experimental animals, which were fed on crops from endemic areas of Keshan disease and non-endemic areas for 8-13 weeks. The absorption and distribution of 75Se (Na2 SeO3) in above tissue and organs were studied. Results revealed that the absorption of Na2-SeO3 in experimental group fed on crops from endemic area was higher than control group fed on crops from non-endemic area. The gradient of distribution of 75Se in various organs was regular: The highest absorption was in kidneys, the lesser was in liver, spleen and thyrnus, the least was in muscle, heart and bones.In one week observation, the contents of 75Se changed as time passed, but the fluctuating changes of the two groups were different.The regular differences of 75Se absorption as above, revealed a distinction between endemic and non-endemic crops. Under the influences of the crops on experimental animals, there appeared more or less a condition of selenium deficiency, which proved that the soil-water-food chain had apparently influenced the selenium metabolism of the organisms.