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OBJECTIVE To explore the mechanism of modified Xianfang huoming decoction in the treatment of sepsis- induced liver injury from the perspective of gut microbiota and metabolites. METHODS Sixty SD rats were divided into blank group （normal saline）， model group （normal saline）， positive control drug group （Dexamethasone tablet， 5.0 mg/kg）， modified Xianfang huoming decoction high-dose， middle-dose and low-dose groups （6.0， 3.0， 1.5 g/kg， calculated by crude drug） according to equilibrium-partitioning approach of body mass， with 10 rats in each group. They were given relevant drug/normal saline 10 mL/ kg， once a day， for consecutive 14 days. After the last medication， except for blank group， other groups were given intraperitoneal injection of lipopolysaccharide 10 mg/kg to induce sepsis model. Twelve hours after modeling， serum levels of inflammatory indexes in rats [interleukin-1β （IL-1β）， IL-6， tumor necrosis factor-α （TNF-α）] and liver function indicators [total cholesterol （TC）， triglyceride （TG）， aspartate transaminase （AST）， alanine transaminase （ALT）] were detected. The changes of gut microbiota and liver metabolites in rats were analyzed by 16S rRNA technology and liver metabolomics. RESULTS Modified Xianfang huoming decoction could significantly improve the indexes of serum inflammatory indexes and liver function in rats with sepsis-induced liver injury （P＜0.05 or P＜0.01）； there was a significant callback effect on the relative abundance of 11 genera of bacteria （such as Akkermansia， Lactobacillus and Bilophila） among the 5 dominant phyla（P＜0.05 or P＜0.01）. Twelve metabolites related to liver injury caused by sepsis were identified， such as glycine cholic acid， phosphatidylcholine， taurine （P＜0.05 or P＜0.01）， mainly involving glycerol phospholipid metabolism， purine metabolism and primary bile acid metabolism. CONC LUSIONS Modified Xianfang huoming decoction can improve liver injury induced by sepsis by regulating gut microbiota and liver metabolites.
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During the development of science of acupuncture and moxibustion, the acupuncture monographs in Song Dynasty had been respected by scholars. Among those monographs, Tongren Shuxue Zhenjiu Tujing, which was compiled by WANG We-iyi in accordance with the emperor's command in Northern Song Dynasty, collected and collated the doctrine of the past, revised its error, designed and casted the bronze portrait as a model simulation, textually researched on meridian theory, and made a huge contribution for the development of acupuncture and standardization of acupuncture teaching and physician examination. In Southern Song Dynasty, WANG Zhi-zhong compiled the Zhenjiu Zisheng Jing. In this book those who met the traditional Chinese medicine and acupuncture theory and heired own clinical experiences were recorded together with practical guide on clinic. This book was instructive and meaningful to the clinical practice. This paper analyzed the lingual and cultural characters of these two reprehensive acupuncture monographs of Song Dynasty in a new viewpoint, and revealed the irreplaceable literature value, clinical value and cultural value of acupuncture monographs in Song Dynasty from their practicality, inheritance and innovativeness.