RESUMO
Objective To investigate the influence of astragalus polysaccharide administration on the the expression of pancreatic Kir6.2 in diabetes rats.Methods Wistar rats were induced diabetes mellitus,then divided into model group,control group,low dose group,and high dose group randomly.After OGTT of them were observed,determination of the Kir6.2 mRNA and protein expression levels in pancreas was performed by RT-PCR and Western blotting.Results The expression of Kir6.2 in model group decreased significantly,and the expressions of Kir6.2 in low dose and hish dose groups were higher than the model group (P<0.05).Conclusion Astragalus polysaccharide displayed up-regulation to the expression of pancreatic Kir6.2.which may be a mechanism on reducing blood glucose.
RESUMO
Objective To investigate the effect of Xinhuang Pian on pancreatic apoptosis and the protein expression of Bcl-2 and c-Myc in rat with acute pancreatitis (AP). Methods 90 Wister rats were randomly divided into 3 groups, including control group, AP group and Xinhuang Pian treatment (XP) group.AP model was induced by pancreaticobiliary injection of 5% sodium taurocholate (1ml/kg weight). Rats in XP group were given Xinhuang Pian solution (480 mg/kg weight) lavage 30min after AP induction, The rats were sacrificed at 6, 12, 24 h after AP induction, and serum amylase levels were determed, pancreatic pathological change was observed; apoptosis was determined by TUNEL method. The determination of Bcl-2and c-Myc protein expression was performed by Western blot. Results 12 h after AP induction, serum amylase, pancreatic pathological scores, apoptotic indexes and the protein expression of Bcl-2 and c-Myc were (1927 ± 186) U/L, 0, 0.59 ± 0. 12, 0.406 ± 0. 112, 0. 185 ± 0. 046 in control group; (5611 ± 473) U/L,2.65 ± 0. 56, 3.80 ± 0. 91, 0. 282 ± 0.082, 0. 339 ± 0. 076 in AP group; and (4572 ± 400) U/L, 2.40 ± 0. 75,7.15 ± 0. 86, 0. 220 ± 0.068, 0. 302 ± 0. 090 in XP group. The levels of serum amylase, pancreatic pathological scores, apoptotic indexes and the protein expression of c-myc in AP and XP group were all significantly higher than those in control group (P < 0. 05), while the protein expression of Bcl-2 was significanfiy lower than that in control group ( P < 0.05 ). Compared with AP group, the levels of serum amylase, pancreatic pathological scores, protein expression of Bcl-2 were all significanfiy lower in XP group (P < 0. 05 ), but the apoptotic indexe was significantly higher (P < 0. 05 ), and there was no significat change of expression of c-Myc protein.Conclusions Xinhuang Pian may induce the pancreatic apoptosis through down-regulating Bcl-2 protein expression, and result in the alleviation of pancreatic inflammatory reaction.
RESUMO
By analyzing "Diagnose and treatment of cholera in Shanghan Lun (Treatise on Febrile Diseases)", we found that such symptoms as serious vomiting and dysentery appeared in febrile diseases, which is similar to cholera. Therefore, Shanghan Lun named it "cholera illness". This name not only clearly manifested the characteristics of such gastrointestinal-febrile disease, but also emphasized the significance of differential diagnosis between cholera with "cholera illness" in febrile diseases.