RESUMO
Abstract Objective: Despite the literature on dydrogesterone, studies on dydrogesterone utilization patterns are largely lacking in Indian patients. Methods: This was a multi-center, retrospective, observational, cross-sectional, and descriptive study across 817 centers in India. Data of patients who received dydrogesterone in past and provided consent for future use of their medical record for research purpose was were retrieved and analyzed. Results: Data of 7287 subjects (aged 29.55±4.84 years) was analyzed. Threatened abortion was the most common indication for which the subjects received dydrogesterone (46.9%) followed by recurrent pregnancy loss. Polycystic ovary syndrome (PCOS), thyroid disorders and anemia were the most common comorbid conditions and prior pregnancy loss, advanced maternal age and obesity were the most common risk factors seen in subjects who received dydrogesterone. Total 27.5% of subjects received a loading dose of dydrogesterone, and majority (64%) received 40 mg as loading dose. 10 mg dose was used as maintenance or regular dose in 81.4% of the subjects. Twice daily (BID) was the most common dosing frequency (66.6%). The most common concomitant medications being taken by the subjects on dydrogesterone included folic acid (45.1%), iron supplements (30.3%) and calcium and vitamin D3 supplements (25.5%). Another progesterone preparation (oral, injection, vaginal, tubal) other than dydrogesterone was used concurrently in 7.8% of subjects. Conclusion: The study helped to identify the patient population that is benefitted by dydrogesterone and the preferred indications, risk factors, comorbid conditions and concomitant medication used in this patient population at real-life scenario.
Assuntos
Humanos , Feminino , Comorbidade , Fatores de Risco , Ameaça de Aborto , DidrogesteronaRESUMO
Methods@#This study included 25 patients with adult lytic HGSL (Meyerding grade III and IV) operated using a hybrid technique from 2012 to 2015. Data were compared with retrospective data on conventional open reduction (n=23) operated from 2000 to 2015. The minimum follow-up was 5 years. Clinical outcomes were assessed using the Visual Analog Scale (VAS) score and modified Oswestry Disability Index (m-ODI). The spinopelvic and perioperative parameters were recorded. The inter-body fusion and adjacent segment degeneration (ASD) were assessed on radiographs at the final follow-up. @*Results@#The average age in the MTFD and open groups was 45.84±12.70 years (nine males and 16 females) and 49.26±13.33 years (eight males and 15 females), respectively. Further, 22 and three patients in the MTFD group and 19 and four in the open group had grade III and IV listhesis, respectively. The MTFD group demonstrated less operative time, blood loss, and hospital stays than the open group. Significant improvements were observed in VAS and m-ODI in subsequent follow-ups in both groups. The MTFD group fared better at 3 months but outcomes were comparable at the final follow-up. Both techniques were equally effective in restoring spinopelvic parameters. The incidence of ASD is comparable. @*Conclusions@#The technique was proven effective in reducing HGSL. The long-term clinical and radiological outcomes were favorable and comparable with the conventional approach.
RESUMO
Abstract Objective: Horizontal semicircular canal site pathology of benign paroxysmal positional vertigo demonstrating three types of nystagmi on positional test were studied. We have attempted to design a protocol for its diagnosis and treatment. Methods: 320 patients of HSC-BPPV were subjected to two types of positional tests. Of these, patients with bilateral steady apogeotropic nysatgmus were treated with VAV modification of Semont's maneuver. Patients with unsteady or changing apo/geotropic signs were converted into steady geotropic ones by repetitive positional tests; followed by barbecue maneuver with forced prolong positioning. Results: Overall 88% of patients had a total recovery. 92% of patients with geotropic nystagmus showed no symptoms after second maneuveral sitting. 85% of patients with apogeotropic nystagmus recovered fully after third maneuveral sitting. Conclusion: Correct identification of subtypes of HSC-BPPV is based on provoked nystagmus by positional tests. After locating the site and side on the basis of nystagmic pattern, physician can apply the appropriate PRM. Level of evidence: II a.
RESUMO
Objective: In general, proximal restorations of primary molars fracture, so it is vital to study the new materials that could solve this problem. Hence, the present trial assessed the success of occluso-proximal atraumatic restorative treatment (ART) restorations using silorane and glass ionomer cement (GIC) in carious primary molars for a period of 2 years. Materials and Methods: One hundred and ninety-two children between the age group of 4 and 9 years were randomly allocated to GIC or silorane. In the clinical set up, they were treated by a pediatric dentist, and their restorations were evaluated at 3, 6, 12, 18, and 24 months. The primary outcome was the survival of restoration, which was analyzed using the Kaplan–Meier and superior Cox regression models. As a sensitivity analysis, intention-to-treat (ITT) was executed. Sex, age, molar, jaw, cavity volume, and caries incidence were the independent variables. Results: The restoration survival after 24 months for GIC and silorane was 82.75% and 88.88%, respectively, whereas ITT analysis showed a success of 84.37% and 89.58% for GIC and silorane, respectively. Conclusion: With regard to longevity, there was no statistically significant difference between silorane and GIC in primary molar occlusoproximal ART restorations.
RESUMO
Effect of non-thermal atmospheric pressure plasma and ErCr YSGG LASER activation of three fluoride varnishes on surface re-mineralization of enamel A SEM-EDX analysisBackground: Dental remineralization is the process of transporting minerals from the surrounding environment (i.e., saliva and biofilm) into partially demineralized tooth structures. Remineralization can be induced by professional therapies such as fluoride-based treatments that have the highest level of supporting evidence. High-intensity LASER and nonthermal atmospheric pressure plasma therapy have been known to increase the resistance of enamel to demineralization by surface modification. Aim: The aim of the study was to evaluate and compare the surface remineralization of enamel using ErCr:YSGG LASER and nonthermal atmospheric pressure plasma (NTP) activation with three different fluoride varnishes. Methodology: Sixty-eight extracted premolars were used which were sectioned mesiodistally to obtain 135 specimens and artificial caries were induced on the experimental surface. They were then randomly divided into three groups (n = 45): MI Varnish (GC Japan), Vanish Varnish (3M ESPE), and Embrace Varnish (Pulpdent). After varnish application, these groups were further divided into three subgroups based on the activation therapy used. Fifteen samples from each group were treated with ErCr:YSGG LASER, 15 samples with NTP, and 15 samples were the control that did not undergo activation. After 9 days of pH cycling, the mean ion concentration of the surface calcium and phosphate ions was recorded using FEG-SEM and EDX analysis. The data were statistically analyzed. Results: One-way ANOVA and post hoc Tukey test accepting P < 0.05 were performed for comparisons between all analyses groups. Vanish Varnish showed a higher Ca/P ratio in LASER, NTP, and control subgroups, followed by MI Varnish and Embrace Varnish. ErCr:YSGG LASER therapy showed an improved Ca/P ratio in all varnishes than NTP therapy and control. Conclusion: ErCr:YSGG LASER therapy showed positive effects toward improving the Ca/P, followed by NTP therapy as compared to control in all three varnishes indicating their role in enhancing the effects of remineralization. Vanish Varnish showed a higher Ca/P ratio indicating better remineralization post activation.
RESUMO
To gain insights on the diverse practice patterns and treatment pathways for prostate cancer (PC) in India, the Urological Cancer Foundation convened the first Indian survey to discuss all aspects of PC, with the objective of guiding clinicians on optimizing management in PC. A modified Delphi method was used, wherein a multidisciplinary panel of oncologists treating PC across India developed a questionnaire related to screening, diagnosis and management of early, locally advanced and metastatic PC and participated in a web朾ased survey (WBS) (n = 62). An expert committee meeting (CM) (n = 48, subset from WBS) reviewed the ambiguous questions for better comprehension and reanalyzed the evidence to establish a revote for specific questions. The threshold for strong agreement and agreement was ?90% and ?75% agreement, respectively. Sixty?two questions were answered in the WBS; in the CM 31 questions were revoted and 4 questions were added. The panelists selected answers based on their best opinion and closest to their practice strategy, not considering financial constraints and access challenges. Of the 66 questions, strong agreement was reached for 17 questions and agreement was achieved for 22 questions. There were heterogeneous responses for 27 questions indicative of variegated management approaches. This is one of the first Indian survey, documenting the diverse clinical practice patterns in the management of PC in India. It aims to provide guidance in the face of technological advances, resource constraints and sparse high?level evidence.
RESUMO
Myocardial infarction (MI) is the leading cause of death from coronary heart disease and requires immediate reperfusion therapy with thrombolysis, primary percutaneous coronary intervention, or coronary artery bypass grafting. However, myocardial reperfusion therapy is often accompanied by cardiac ischemia/reperfusion (I/R) injury, which leads to myocardial injury with detrimental consequences. The causes of I/R injury are unclear, but are multifactorial, including free radicals, reactive oxygen species, calcium overload, mitochondria dysfunction, inflammation, and neutrophil-mediated vascular injury. Mild hypothermia has been introduced as one of the potential inhibitors of myocardial I/R injury. Although animal studies have demonstrated that mild hypothermia significantly reduces or delays I/R myocardium damage, human trials have not shown clinical benefits in acute MI (AMI). In addition, the practice of hypothermia treatment is increasing in various fields such as surgical anesthesia and intensive care units. Adequate sedation for anesthetic procedures and protection from body shivering has become essential during therapeutic hypothermia. Therefore, anesthesiologists should be aware of the effects of therapeutic hypothermia on the metabolism of anesthetic drugs. In this paper, we review the existing data on the use of therapeutic hypothermia for AMI in animal models and human clinical trials to better understand the discrepancy between perceived benefits in preclinical animal models and the absence thereof in clinical trials thus far.
RESUMO
Background: Composites are the widely used restorative materials, and over the year, newer restorative composites have been introduced to eliminate the drawbacks of previous ones. The recent advance in restorative dentistry is bioactive restorative materials. However, bacterial plaque formation on these restorations is the primary reason for secondary caries. Aims and objectives: The purpose of this study was to do the comparative evaluation of bioactive restorative composites (Beautifil Flow Plus, Activa BioACTIVE, and Filtek Z250 XT as control) for their antibacterial efficacy under in vitro conditions. Materials and Method: Thirty material blocks were used for this evaluation. Antibacterial efficacy was checked against Streptococcus mutans and observed under confocal laser scanning microscopy. Results: The results showed that Activa BioACTIVE shows maximum number of dead bacteria on the material surface compared to other groups. Conclusion: It can be concluded as it has maximum antibacterial efficacy among tested materials.
RESUMO
Introduction: Surgery and anesthesia can be a traumatic experience for a child. Stormy induction of anesthesia in children canlead to an increased incidence of post-operative behavioral problems. Thus, sedative premedication may be used in childrento aid smooth induction of anesthesia. Hence, this study was conducted to compare the preanesthetic sedative effects ofintranasal midazolam and ketamine.Materials and Methods: This study was carried out on 60 ASA Grades I and II pediatric patients aged 2–5 years undergoingemergency or elective surgery lasting for 30 min–2 h. The exclusion criteria were established. Patients were included after writteninformed consent of the parent/guardian. They were randomly divided into two groups: Group M and Group K. Pre-operative,intraoperative, and post-operative parameters were observed.Results: Statistically significant tachycardia and increased blood pressure (BP) (both clinically insignificant) were observed inboth the groups (more persistent in the ketamine group). The sedation by both the drugs was adequate in terms of parentalseparation score, acceptance of facemask and response to venipuncture with no statistically significant difference. There werealso no significant side effects (namely, respiratory depression, increased secretions or emergence reaction).Conclusion: Both the drugs midazolam and ketamine provide adequate preanesthetic sedation through intranasal route andare safe to use in pediatric patients without any significant side effects.
RESUMO
The occurrence of oligohydramnios complicating a pregnancy is seen in 0.8 to 5.5 % of pregnancies. Severe Oligohydramnios, though not clearly defined, but clinically with an AFI of less than 5 cm appears to be an important predictor for an abnormal fetal outcome. In general, the prognosis of mid trimester oligohydramnios is still poor. Hence we report a case with a very favourable neonatal outcome following severe oligohydramnios documented in early pregnancy. The aim of this case report is to add our experience to the currently limited literature regarding the best treatment of this unique obstetrical problem.
RESUMO
@#The introduction of the recent 2017 classification of periodontal diseases has been welcomed by the periodontal fraternity. The new classification has used available evidence as the backbone for its development. It has been felt by many experts that a good understanding is required for its practice. Towards this, a Malaysian PerioSymposium was organised in May 2019 to comprehend the classification and also to identify the right path towards its learning among undergraduate students of dentistry in Malaysia. The symposium was conducted and moderated by local and international experts. Apart from the few limitations identified, the new classification was recommended to be taught in the undergraduate dental programme across schools in Malaysia.
RESUMO
Objective:To investigate L-3,4-dihydroxyphenylalanine (L-dopa,anfi-Parkinson drug),anti-inflammatory activity,proximate nutritional composition and antioxidant potential of Mucuna macrocarpa (M.macrocarpa) beans.Methods:L-dopa content was determined and quantified by high performance thin layer chromatography and reversed phase high-performance liquid chromatography (RPHPLC) methods.Anti-inflammatory activity was performed by in vitro protein denaturation inhibition and human red blood cell membrane stabilisation activity.Proximate composition and elemental analysis were also investigated.The antioxidant potential (2,2-diphenyl-1-picrylhydrazyl,N-N-dimethyl-phenylenediamine and ferric-reducing antioxidant power) of M.macrocarpa beans were evaluated by using different extraction solvents.The RP-HPLC analysis also quantified significant phenolics such as gallic acid,tannic acid,p-hydroxybenzoic acid and p-coumaric acid.Results:RP-HPLC quantification revealed that M.macrocarpa beans contain a high level of L-dopa [(115.41 ± 0.985) mg/g] which was the highest among the Mucuna species from Indian sub-continent.Water extract of seed powder showed strong antiinflammatory and antioxidant potential.Proximate composition of M.macrocarpa beans revealed numerous nutritional and anti-nutritional components.RP-HPLC analysis of major phenolics such as tannic acid (43.795 mg/g),gallic acid (0.864 mg/g),p-coumafic acid (0.364 mg/g) and p-hydroxybenzoic acid (0.036 mg/g) quantified successfully from M.macrocarpa beans respectively.Conclusions:This study suggests that M.macrocarpa is a potential source of L-dopa with promising anti-inflammatory,antioxidant and nutritional benefits.
RESUMO
Objective To investigate L-3, 4-dihydroxyphenylalanine (L-dopa, anti-Parkinson drug), anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa (M. macrocarpa) beans. Methods L-dopa content was determined and quantified by high performance thin layer chromatography and reversed phase high-performance liquid chromatography (RP-HPLC) methods. Anti-inflammatory activity was performed by in vitro protein denaturation inhibition and human red blood cell membrane stabilisation activity. Proximate composition and elemental analysis were also investigated. The antioxidant potential (2,2-diphenyl-1-picrylhydrazyl, N-N-dimethyl-phenylenediamine and ferric-reducing antioxidant power) of M. macrocarpa beans were evaluated by using different extraction solvents. The RP-HPLC analysis also quantified significant phenolics such as gallic acid, tannic acid, p-hydroxybenzoic acid and p-coumaric acid. Results RP-HPLC quantification revealed that M. macrocarpa beans contain a high level of L-dopa [(115.41 ± 0.985) mg/g] which was the highest among the Mucuna species from Indian sub-continent. Water extract of seed powder showed strong anti-inflammatory and antioxidant potential. Proximate composition of M. macrocarpa beans revealed numerous nutritional and anti-nutritional components. RP-HPLC analysis of major phenolics such as tannic acid (43.795 mg/g), gallic acid (0.864 mg/g), p-coumaric acid (0.364 mg/g) and p-hydroxybenzoic acid (0.036 mg/g) quantified successfully from M. macrocarpa beans respectively. Conclusions This study suggests that M. macrocarpa is a potential source of L-dopa with promising anti-inflammatory, antioxidant and nutritional benefits.
RESUMO
In the present study, we aimed to estimate the occurrence of bovine tuberculosis [TB] and examine the determinants of distribution of the disease in three high-risk populations of Central India. A prospective cohort study was conducted in Central India between March 2014 and June 2015. Based on the requisite inclusion criteria, we recruited a total of 301 participants whose blood samples were subjected to poly-merase chain reaction-based detection and differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. M. bovis was detected in 11.4%, 8.9%, and 12.6% of the recruited participants belonging to three distinct population groups [Groups A, B, and C, respectively]. The highest proportion of cases infected with M. bovis was observed in Group C, who lived in the high TB endemic region. Previous contact with active TB cases [odds ratio = 3.7; 95% confidence interval, 0.9612-14.4533] and raw milk consumption [odds ratio = 5.3472; 95% confidence interval, 1.9590-14.5956] were found to be important determinants of bovine TB in this population. The high incidence rates of bovine TB in the Central Indian populations indicate the substantial consequences of this disease for some population groups and settings. However, more research is necessary to identify the main transmission drivers in these areas
Assuntos
Humanos , Criança , Zoonoses , Prevalência , Fatores de Risco , Estudos Prospectivos , Estudos de Coortes , Mycobacterium bovis , Mycobacterium tuberculosisRESUMO
The tuberculin skin test [TST] and interferon-gamma release assays [IGRA], namely, the QuantiFERON-TB Gold test [QFT], remain the standard immuno-logical diagnostic tools for latent tuberculosis [TB] infection [LTBI]. However, the sub-optimal detection rates of both of these tests are major impediments in recognizing the population at risk. This study was aimed at evaluating additional cytokines besides interferon-gamma [IFN-gamma] as biomarkers for improving LTBI diagnosis in the tribal population of Melghat, India. Seventy-four close TB contacts were stratified by QFT and TST results into: [i] QFT+/TST+ [n = 26], [ii] QFT+/TST- [n = 12], [iii] QFT-/TST- [n = 35] and [iv] QFT-/TST+ [n = 1] groups. A panel of cytokines [IL-6, IL-10, TNF-alpha and IL-2R] was then evaluated in antigen-stimulated QFT cell-free culture supernatants using IMMULITE-1000, an automated immunoassay analyzer. Cytokine estimation showed significantly higher levels of IL-6 in the QFT+/TST+ group, while significantly higher levels of IL-10 were found in the QFT-/TST- group. Correlation analysis identified a positive correlation between IL-6 and the QFT response [r = 0.6723, P< 0.0001], while a negative correlation was seen between QFT and IL-10 expression [r=-0.3271, P = 0.0044]. Similarly, IL-6 was positively correlated with TST levels [r = 0.6631, P< 0.0001], and conversely, a negative correlation was found between TST and IL-10 expression [r=-0.5698, P<0.0001]. The positive and negative predictive values of IL-6 were found to be 92.59 and 93.33%, respectively, and the positive and negative predictive values of IL-10 were 96.55 and 91.18%, respectively. No significant impact of the demographic characteristics on cytokine positivity was observed. Our preliminary results suggest that the evaluation of additional cytokines in QFT cell-free culture supernatants may be valuable for the identification of LTBI. Combining IL-6 and IL-10 with QFT and/or TST could markedly improve the detection accuracy of LTBI. Our observations require investigation in larger well-characterized cohorts along with follow-up studies to further confirm the study outcome
RESUMO
Rubinstein–Taybi syndrome (RSTS), a developmental disorder comprising abnormalities that include mental retardation, an unusual facial appearance, broad thumbs and big toes is frequently associated with molecular lesions in the CREB-binding protein gene, CREBBP. The objective of the present study was to identify and analyse CREBBP mutations in Indian RSTS patients on which there are no data. Direct sequencing of CREBBP performed in 13 RSTS patients identifi ed the three zinc fi ngers (CH1, CH2, CH3) and HAT domain as mutational hotspots in which ten novel pathogenic mutations were localized. Functional analysis revealed that three of these mutations affecting amino acids Glu1459, Leu1668 and Glu1724 were critical for histone acetyltransferase activity. Twenty-eight novel CREBBP single-nucleotide polymorphisms (SNPs) were also identifi ed in the Indian population. Linkage disequilibrium studies revealed associations between (i) SNP (rs129974/c.3836-206G>C) and mutation (p.Asp1340Ala); (ii) (rs130002) with mutation (p.Asn435Lys) and (iii) SNPs rs129974, rs130002 and SNP (c.3836-206G>C) signifying a disease affection status. In conclusion, the present study reports the highest detection rate of CREBBP mutations (76.9%) in RSTS patients to date, of which ten are predicted to be pathogenic and three critical for histone acetyltransferase activity. Moreover, identifi cation of the association of CREBBP polymorphisms with disease susceptibility could be an important risk factor for the pathogenesis of RSTS.
RESUMO
Background : Diarrheal diseases affect millions of people around the world and have the greatest impact on children, especially those in developing countries. Rotavirus is now known to contribute significantly to the etiology of diarrheal diseases in both developing and developed countries. Objective : To study demographic, clinical, and laboratory characteristics of rotavirus infection in cases of acute diarrhea admitted in the hospital who were below the age 5 years. Materials and methods : It was a prospective study conducted from June 2009 to June 2011 to determine characteristics, clinical profile, and antigenemia of rotavirus. The study group included patients up to 5 years of age hospitalized with a diagnosis of acute gastroenteritis. Statistical analysis was done using the chi-square test. Data were analyzed using SPSS (Statistical Package for Social Sciences) version 17.0. Results : From the 246 cases studied in the hospitalized patients of acute gastroenteritis, 88 cases (35.77%) were found to be positive for rotavirus infection. The occurrence of infection was found to be most common in the age group of 11-20 months (38.63%) followed by the age group of 0-10 months (23.86%). There was no association with the sex of the child. In rotavirus-positive patients, associated features were fever in 48.86%, vomiting in 29.54%, and pain in abdomen in 53.4% of cases. Seasonal variation of rotavirus infection was observed in the present study with peak in the winter season. It was found that 5.68% cases which were positive for rotavirus in their stool samples were positive for rotavirus present in their urine sample, suggesting extraintestinal manifestation of rotavirus. Conclusion : It is concluded that in spite of clean hygienic condition and vaccination rotavirus is still an important cause of infantile diarrhea in urban area like Pune. There is association between the occurrence of rotavirus and degree of dehydration, fever, vomiting, and pain in abdomen. Peak incidence was found in the months of December and January. There is association between the occurrence of rotavirus in stool and rotavirus in blood.
Assuntos
Grupos Etários , Criança Hospitalizada , Feminino , Gastroenterite/epidemiologia , Gastroenterite/etiologia , Humanos , Incidência , Lactente , Recém-Nascido , Índia/epidemiologia , Masculino , Infecções por Rotavirus/complicações , Infecções por Rotavirus/epidemiologiaRESUMO
Aim: Gallstone disease is the most common cause of acute pancreatitis. Cholecystectomy is mandatory to avoid recurrence of pancreatitis. Our objective was to evaluate the results of laparoscopic cholecystectomy (LC) in patients with gall-stone induced pancreatitis . Methods: All patients presenting to us within the time frame from February 2004 to June 2008 with acute biliary pancreatitis were included in the study. The severity of pancreatitis was assessed by Ranson’s criteria. ERCP and endoscopic sphincterotomy was performed when the common bile duct (CBD) was dilated (>6 mm) with either calculi or sludge as seen on imaging. Patients with successful ERCP with predicted demanding laparoscopic cholecystectomy were discharged instead for an elective LC, 4-6 weeks later. Patients with mild pancreatitis (with Ranson’s score of 3 or less) and predicted uncomplicated LC underwent surgery at the same admission. The difficulty of the procedure was determined by the presence of adhesions in the gallbladder area, dissection in Calot’s triangle, tackling the dilated cystic duct, intra-operative bleeding, and the need for a drain. Results: A total of 26 patients (12 male and 14 female; age range 23-75 years) with acute biliary pancreatitis comprised the study group. Eleven patients with suspected choledocholithiasis underwent ERCP and clearance of the CBD was done in all of them. Nine patients (2 ERCP and 7 non-ERCP) underwent early LC in the same admission. Seventeen patients (9 ERCP and 8 non-ERCP) were predicted as difficult cases for LC and underwent delayed LC. No patient had recurrent pancreatitis in the interval period. Conclusion: There was no significant difference in the operative difficulty between early and delayed LC when patients were selected for timing of LC based on pre-defined criteria.
RESUMO
OBJECTIVE: To evaluate the predictive value of a cord serum screen test and possible subsequent development of allergic disease in infants. DESIGN: Cohort study. SETTING: 100 pregnant women were randomly recruited for the study. METHODS: The maternal serum and the cord serum of their matched newborn were analyzed for total serum immunoglobulin E (IgE), gamma interferon (g IFN), house dust mite- Dermatophagoides pteronyssinus allergen (Der p1) and Blomia tropicalis allergen (Blo t5) using immunoassay methods. All infants were followed up for one year. RESULTS: Infants who had allergic diseases in the one year follow-up (n=45) had significantly (P < 0.001) elevated IgE, Der p1, Blo t5, and significantly low g IFN levels in cord serum as compared to the same parameters of infants who did not develop allergic disease in the one year follow-up (n=43). CONCLUSION: In utero exposure to HDM allergens Der p1 and Blot5 is prevalent. We have successfully established a cord serum screening test for predicting allergic diseases in infancy with 93% specificity and sensitivity.