RESUMO
The immunogenic potency of anti-leprosy ICRC vaccine preparations was determined by foot pad enlargement in mice. Female Balb/c mice were sensitized by I.D. route with irradiated, heat-killed or live antigens of 2 strains (C-44 and C-75) of ICRC bacilli and tested against lepromin and ICRC-antigens. ICRC antigens sensitized mice against lepromin; similarly, BCG and M. leprae sensitized mice, which showed good cross reactivity with ICRC antigens. A mixture of irradiated ICRC bacilli and live BCG was not more immunogenic to mice than its single constituents. The immunity induced by ICRC antigens could be adoptively transferred to syngeneic recipients by sensitized spleen cells.
Assuntos
Animais , Vacina BCG/imunologia , Vacinas Bacterianas/imunologia , Feminino , Hipersensibilidade Tardia , Imunidade Celular , Imunização , Imunização Passiva , Camundongos , Mycobacterium leprae/imunologia , Baço/imunologiaRESUMO
Leucocyte migration inhibition, in presence of specific particulate antigen, is a good correlate of CMI. It can detect small differences in related antigens. In the present study, LMI was used to study the cross-reactivity between ICRC bacilli and M. leprae and also to examine the antigenic relationship amongst different ICRC isolates. The study showed a close antigenic cross-reactivity in lepromin and ICRC-in. LMI has brought out strain differences in two ICRC culture isolates, C-44 and C-75. The data appear to elucidate the possible strain specific antigenic relationship of M. leprae with its culture isolate e.g. ICRC strains. This may have important bearing on the development effective antileprosy vaccine for human use.
Assuntos
Animais , Vacina BCG/imunologia , Vacinas Bacterianas/imunologia , Inibição de Migração Celular , Reações Cruzadas , Feminino , Antígeno de Mitsuda/imunologia , Hanseníase/microbiologia , Leucócitos/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Mycobacterium/imunologia , Mycobacterium avium/imunologia , Mycobacterium leprae/imunologia , Baço/imunologiaRESUMO
ICRC-bacilli strain C-44 when grown in Dubos medium of its equivalent, express M. avium taxonomic biochemical characters. Assuming that difference in characters of M. leprae and ICRC bacilli, could be due to 'in vivo' and 'in vitro' milieu, we altered the substrates in the medium. The bacilli grow well in the new medium containing selenium, ferric nitrate, magnesium chloride and deleting Tween 80. The ICRC strain C-44 grown in new medium expressed characters: 9/10 similarity with M. leprae. The 10 day tween hydrolysis reaction in weak but positive. It is probable that 'M. leprae culture isolate', may have acquired 'in vitro' growth potential by recombination with M. avium, an ubiquitous mycobacterium. The M. leprae culture isolate thus may express some characters of both M. leprae and M. avium.
Assuntos
Meios de Cultura , Mycobacterium/metabolismo , Mycobacterium avium/classificação , Mycobacterium leprae/classificaçãoRESUMO
Presence of O-phenoloxidase is regarded as M. leprae specific character. This enzyme activity was found to be present in ICRC bacilli, Strain C-44. Though this strain is cultivable 'in vitro', the expression of DOPA-Oxidase activity strongly suggests that it carries M. leprae genome. The ICRC bacilli, therefore, may thus from a group of M. leprae culture isolates, distinct from other known cultivable mycobacteria which do not possess this enzyme activity.
Assuntos
Catecol Oxidase/metabolismo , Humanos , Hanseníase/microbiologia , Monofenol Mono-Oxigenase/metabolismo , Micobactérias não Tuberculosas/enzimologia , Mycobacterium avium/enzimologia , Mycobacterium leprae/enzimologia , Especificidade da EspécieAssuntos
Mostarda de Anilina/análogos & derivados , Neoplasias da Mama/tratamento farmacológico , Feminino , Glucuronatos/uso terapêutico , Glucuronidase/metabolismo , Humanos , Neoplasias Bucais/tratamento farmacológico , Compostos de Mostarda Nitrogenada/uso terapêutico , Técnicas de Cultura de Órgãos , Neoplasias Ovarianas/tratamento farmacológicoAssuntos
Adolescente , Adulto , Antineoplásicos/uso terapêutico , Criança , Cobre/sangue , Quimioterapia Combinada , Feminino , Doença de Hodgkin/sangue , Humanos , MasculinoRESUMO
Three fold increase of arginase activity was observed in hydrocortisone treated mammary tumour tissue when compared to the untreated tissue in vitro. No change in succinic dehydrogenase activity was observed. It is likely that arginase present in mammary tumour is due to the presence of mammary tumour virus and it is tempting to speculate that the increase in arginase activity by hydrocortisone may be due to sustained viral production in the presence of hydrocortisone.