Detection of enterotoxigenic Clostridium perfringens type: a in camel meat

Clostridium perfringens enterotoxin [CPE] is an important sporulation associated virulence factor in several illnesses of humans and domestic animals. In the present investigation 169 camel meat samples were collected from small butcher's shop- where C. perfringens type A was identified with an incidence of 33.7% only 5.9% had detectable enterotoxin. The meat samples tested had MPN per gram values ranging from 0 to 35, determination of spore heat resistance for cpe positive C. perfringene type A isolates varied between logs 1.3 to log 5.07 at different time intervals. The current study has used PCR assays to detect cpe production in gene as a method for determining the enterotoxigenicity of C. perfringens isolates. Results showed that PCR is suitable for detection of C. perfringens enterotoxin from raw meat

Clostridium sordellii as a cause of enteritis in lambs

During 2004-2005, out of 25 lambs up to 10 weeks old which died suddenly, 13 Clostridium sordellii were isolated. The post- mortem findings varied. Losses have been sporadic in some farms in Kafer el-Sheikh governorate and recorded in all age of lambs. Also, 61 fecal samples of diarrheic lambs at three different age groups, were examined bacteriological ly for the presence ofclostridia with special emphasis on C. sordellii, the isolation rate of C. sordellii was higher in all age groups [24.6, 8.3, and 5%] than other Clostridium species. C.perfringens, C. sporogenes, and C. bifermentans were isolated and identified in a different percentage. In vitro, the sensitivity of C. sordellii isolates to different chemotherapeutic agents was determined .Results of this study indicates that Clostridium sordellii should be considered when investigating the cause of sudden death in lambs

Effect of pediococcus acidilactici and saccharomyces boulardii as probiotics on intestinal and caecal colonization of salmonella typhimurium and clostridium perfringens in broiler chickens

A significant reduction in intestinal and caecal colonization of Clostridium perfringens [C. perfringens] during 21 days post infection [PI] has occurred on using the probiotics Pediococcus acidilactici or Saccharomyces boiilardii. However; the former resulted in a higher reduction than the latter. The same results were also obtained with Saccharomyces boulardii at 7 and 14 days PI. Reduction in Salmonella typhimurium [S. typhinwrium] caecal colonization significantly happened during the entire period of the experiment [21 days] on using the two studied probiotics. Neither mortality nor disease lesions could be obtained in experimentally infected groups with the used doses of both S.typhimurium and C perfringens under the conditions of the experiment