A Bacteriological of Make-up Tools Used in Calabar Metropolis, Cross River State, Nigeria

Sharing of make-up tools by multiple users is a possible means of transfer of fungal infections, such as dermatophytoses. With abrasion on the skin, it is possible for HIV, Hepatitis B virus, Spirochaetes and other pathogens to be carried via contaminated make-up tools, from one person to another. Using sterilized swab sticks, surfaces of make-up tools (sponge and brush) were cleansed. Materials deposited on the swab sticks were cultured aerobically on Chocolate and Cysteine Lactose Electrolyte Deficient Agar plates overnight at 37oC. After overnight aerobic incubation, the culture plates were read macroscopically for growth. Bacterial isolates were subjected to microscopic examination using Gram’s staining technique and biochemical tests (e.g. coagulase, catalase, oxidase, and motility). Results showed that the predominant bacterial growths obtained from the make-up tools were Staphylococcus aureus (51.5%), Coagulase-negative Staphylococcus (25.8%), Klebsiella pneumoniae (6.2%), and Pseudomonas aeruginosa (16.5%). The different genera of bacteria were harvested from make-up brushes (66.2%) and make-up sponges (85.7%). There was no bacterial growth from 33.8% and 14.3% of make-up brushes and make-up sponges respectively. There was no statistical difference in terms of bacterial growth between the two tools (brush and sponge) sampled (P>0.05). This study has shown that there is a moderate possibility for the transfer of bacterial organisms (both skin flora and pathogens) from one person to another, through make-up tools, in our local communities. It is hereby recommended that health education talks should be carried out regularly among beauticians to encourage them to use disposable make-up tools with disinfectants.

Studies on the Antibacterial Susceptibility of Uropathogens to Senna alata Extracts in Calabar, Cross River State, Nigeria

Background: Senna alata is an underutilized shrub found in many countries and is known for its traditional use in the treatment of dermatophytes and other related diseases. Therefore, this study aimed at evaluating the phytochemical and antibacterial effects of S. alata leaves extracts against bacterial isolates obtained from urinary tract infection patients in Calabar. Methodology: Matured fresh leaves of Senna alata were collected within Calabar, Cross River state, Nigeria, in May 2022 and identified by a botanist in the Department of Botany, University of Calabar. The leaves of S. alata were extracted with water, methanol and ethyl acetate using maceration and soxhlet methods. Phytochemical analysis was conducted to detect the presence of bioactive compounds using standard methods. The crude extracts of S. alata were investigated for antibacterial properties using agar well diffusion method and mechanisms of antibiosis determined using MBC/MIC ratio. Results: In both methods of extraction, methanol yielded more extracts compared to other solvents. Soxhlet methanol extract (SaMeSh) had the highest (12.21%) percentage yield while maceration ethyl acetate extract (SaEaMa) had the least (4.77%) percentage yield. The phytochemicals assayed revealed the presence of saponins, tannins, flavonoids, anthraquinones, terpenoids and steroids. However, terpenoids was not detected in methanol and ethyl acetate extracts. Senna alata extracts demonstrated broad spectrum of activity against the tested isolates at various concentrations with organic solvents exhibiting the highest antibacterial activity. However, the observed activity varied with respect to concentration of extract and types of organisms. The MIC values ranged from 31.25 to 250 mg/mL and MBC values from 62.5 to 500 mg/mL. The MIC index of the crude extracts against the test uropathogens was ?8. Conclusion: This study indicates that S. alata could be a source of novel antimicrobial agent. Further research is required to isolate, characterize and identify bioactive constituents responsible for the observed activity.

Basal and Squamous Cell Carcinomata on the Same Patients: Report of Two Cases in Aibinos

Background: Skin cancer is the most common cancer among the lightly pigmented individuals in Europe; North America and Australia. It occurs infrequently in darkly pigmented individuals because they have more melanin. Melanin and albinism are a known risk factors for skin cancers in Africans. Those already treated for skin cancer run a risk of developing another skin cancer. Method: Two case reports of albinos who developed basal cell carcinoma (BCC) and squamous cell carcinomata (SCC) later are presented. The two female albinos aged 61 and 21 years had BCC and SCC involving the left arm and the dorsum of the left hand in case1 and involving the face and the medial side of the right elbow in case 2. Both patients had numerous actinic keratoses that indicated chronic exposure to sunlight. Results: The outcome was good for the BCC's as the wounds healed post excision. There was no evidence of recurrence when the patients represented with SCC at 12 and 18 months respectively. Conclusion: Albinos run a risk of developing both BCC and SCC with chronic exposure to sunlight. Health education strategies on the need for early institution of preventive measures and follow up are emphasised as they run a risk of developing another cancer

Kaposi Sarcoma: Changing Trend in Calabar; South Eastern Nigeria

Background/objective: Kaposi sarcoma (KS) is now the most frequently reported malignant skin tumour in some areas of Africa and was endemic in Africa before the advent of the human immunodeficiency virus (HIV) infection. The prevalence has increased with the emergence of HIV infection. The objective of this report is to describe the frequency; current clinical pattern; and anatomic distribution of KS in Calabar; south-eastern Nigeria and compare this with total malignant skin tumour. Method: All the patients with histologic diagnosis of KS presenting to the University of Calabar Teaching Hospital from January 2005 and December 2006 were analyzed as part of the wider study of malignant skin tumorus. Diagnosis of HIV was based upon enzyme linked immunosorbent assay. Results: In our study; there were 11 patients (7 males and 4 females); with a male: female ratio of 1.75: 1. This was the commonest malignant skin tumor (38) followed by squamous cell carcinoma (SCC) (34.5) and the age ranged from 21 - 60 years (mean 42.9years). Nine patients (81.8) were HIV positive including the 4 females (age ranged from 21 -45 years) and 2(18.2) HIV negative; aged 59 and 60years. The lower limb was the commonest site (50). Atypical lesions involved the eyelids/ nose and penis. Conclusion: KS is now the commonest malignant skin tumour in our region with the HIV related KS as the commonest clinical type. Successful prevention and treatment of HIV infection would reduce the prevalence of this tumour

Infertility among Nigerian Couples as seen in Calabar

Objective: This study was conducted to determine the infertility pattern among Nigerian couples in Calabar and to examine the outcome of our management strategies.Methods: A retrospective descriptive design study based on findings from the clinical files of infertile couples presenting at the gynaecology and the male fertility clinics of University of Calabar Teaching Hospital over a five-year period (2001-2005). Data were collected from all the documented clinical and laboratory findings.Results: The obvious causes of infertility constituted 58in females; 30in males and 12in both partners. Primary infertility was found in 69.7of males and 34.5of females and secondary infertility in 30.3of males and 65.5of females. Infection appears to be a strong predisposing factor to infertility in both male and female patients in our study population. Pregnancy was achieved in 24of our female patients who had tubal surgery; while 69of our males who had varicocelectomy had improvement in seminal fluid parameters.Conclusion: Prevention and prompt treatment of infection; particularly the sexually transmitted infections could be beneficial in the reduction of infertility among couples