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Objective:To investigate the clinical characteristics of acute myeloid leukemia with BCR-ABL p210 fusion gene-positive.Methods:The clinical characteristics of a patient diagnosed in the Second Hospital of Jiaxing were analyzed and the related literature was reviewed.Results:BCR-ABL p210 fusion gene and Philadelphia chromosome (Ph) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization (FISH). Imatinib associated with multi-drug intravenous chemotherapy resulted in poor efficacy.Conclusions:Patient with Ph +/BCR-ABL + acute myeloid leukemia is rare with a very poor prognosis. There is no unified standard treatment and the efficacy of tyrosine kinase inhibitors is unclear. Intravenous chemotherapy combined with hematopoietic stem cell transplantation is expected to change the prognosis.
RESUMO
AIM: To control the quality of Er Chen Pill (Pericarpium Citri Reticulatae, Rhizoma Pinellae, Poria, Radix Glycyrrhizae) by determination of limonene by GC. METHODS: HP 6890 GC instrument was employed to determine the content of limonene with column (HP-1 Methyl Siloxane 30m?320?m?0.25?m), detector temperature (250?C), injection temperature (250?C), split ratio (100∶1) and injection amount (1?L). RESULTS: The calibration equation was Y=0.9873X+0.0794(r=0.9995) in the range of 0.45?g～ 1.80?g. The average recovery was 99.6%(RSD=6.9%) and the average content of limonene in the sample was 78.4mg?g -1(RSD=3.2%). Conclusion: The method is simple, quick, and sensitive.