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1.
Chinese Journal of Laboratory Medicine ; (12): 802-811, 2020.
Artigo em Chinês | WPRIM | ID: wpr-871978

RESUMO

Objective:To establish autoverification rules for coagulation tests in multicenter cooperative units, in order to reduce workload for manual review of suspected results and shorten turnaround time (TAT) of test reports, while ensure the accuracy of results.Methods:A total of 14 394 blood samples were collected from fourteen hospitals during December 2019 to March 2020. These samples included: Rules Establishment Group 11 230 cases, including 1 182 cases for Delta check rules; Rules Validation Group 3 164 cases, including 487cases for Delta check; Clinical Application Trial Group 77 269 cases. Samples were analyzed for coagulation tests using Sysmex CS series automatic coagulation analyzers, and the clinical information, instrument parameters, test results, clinical diagnosis, medication history of anticoagulant and other relative results such as HCT, TG, TBIL, DBIL were summarized; on the basis of historical data, the 2.5 and 97.5 percentile of all data arranged from low to high were initially accumulated; on the basis of clinical suggestions, critical values and specific drug use as well as relative guidelines, autoverification rules and limits were established.The rules were then input into middleware, in which Stage I/Stage II validation was done. Positive coincidence, negative coincidence, false negative, false positive, autoverification pass rate, passing accuracy (coincidence of autoverification and manual verification) were calculated. Autoverification rules underwent trial application in coagulation results reports.Results:(1) The autoverification algorisms involve 33 rules regarding PT/INR, APTT, FBG, D-dimer, FDP,Delta check, reaction curve and sample abnormalities; (2)Autoverification Establishment Group showed autoverification pass rate was 68.42% (7 684/11 230), the false negative rate was 0%(0/11230), coincidence of autoverification and manual verification was 98.51%(11 063/11 230), in which positive coincidence and negative coincidence were respectively 30.09% (3 379/11 230) and 68.42%(7 684/11 230); Autoverification Validation Group showed autoverification pass rate was 60.37%(1 910/3 164), the false negative rate was 0%(0/11 230), coincidence of autoverification and manual verification was 97.79%(3 094/3 164), in which positive coincidence and negative coincidence were respectively 37.42%(1 184/3 164) and 60.37%(1 910/3 164); (3) Trialed implementation of these autoverification rules on 77 269 coagulation samples showed that the average TAT shortened by 8.5 min-83.1 min.Conclusions:This study established 33 autoverification rules in coagulation tests. Validation showedthese rules could ensure test quality while shortening TAT and lighten manual workload.

2.
Journal of Jilin University(Medicine Edition) ; (6): 1010-1013, 2018.
Artigo em Chinês | WPRIM | ID: wpr-841853

RESUMO

Objective: To explore the differences in the levels of 25 hydroxy vitamin D3 (vitamin D3) of the asthmatic children with different control levels, to analyze the relationships between the vitamin D3 level and the pulmonary function indexes, and to clarify the effects of vitamin D3 level on the asthma control and the pulmonary function. Methods: A total of 64 asthmatic patients who aged from 6 to 14 years old were selected as asttma group, and then according to the control level, asthma groups were divided into well-controlled group, partially controlled group and uncontrolled group. Eighteen healthy children who aged from 6 to 14 years old were selected as control group. The general materials of subjects in gwo groups were collected. The levels of serum vitamin D3 of the subjects in two groups were detected by Roche electrochemiluminescence, and the pulmonary function indexes (FEV1, FEV1 % pred, FEV1/FVC%, and FVC) were measured by spirometry. The levels serum of vitamin D3 of the subjects in asthma and control groups were analyzed by Wilcoxon rank sum test, the differences of vitamin D3 levels among different control level groups were analyzed by Kruskal-Wallis test, and the relationships between the vitamin D3 level and the pulmonary function indexes were analyzed by Spearman correlation analysis. Results: The serum vitamin D3 level of the children in asthma group was significantly lower than that in control group (P0, 05). The percentage of children with vitamin D3 deficiency in uncontrolled group was significantly higher than that in well-controlled group (P<0. 05). The vitamin D3 level was positively correlated with FEV1 and FEVl/FVC% (r=0. 651, P<0. 01; r= 0. 642, P<0. 01). Conclusion: The level of serum vitamin D3 of the asthmatic children affects the control level of asthma and pulmonary function, so vitamin D3 inefficiency or deficiency should be paid attention in the treatment of asthma. Vitamin D3 supplementation is expected to be an important means of adjuvant treatment for asthma.

3.
Chinese Journal of Immunology ; (12)1985.
Artigo em Chinês | WPRIM | ID: wpr-547335

RESUMO

Objective:To prepare gACE polyclonal antibody for functional study of gACE.Methods:According to the bioinformatics analysis and prediction of the possible conformational structure,hydrophobicity and antigenicity of gACE and the principal for antibody production,a partial peptide with 18-amino acid residues of gACE was synthesized after homology search.The synthesized peptide was then used to immunize after coupling with KLH.The properties of anti-gACE were analyzed by ELISA,Western blot and immunohistochemistry.Results:The antigenicity was repredicted by bioinformatics analysis.The polyclonal antibody against gACE was successfully obtained and its specificity and sensitivity we conformed by ELISA,Western blot and immunohistochemistry.Conclusion:By the bioinformatics analysis and prediction,the hydrophilicity and antigenicity of gACE are analyzed.The antibody of gACE is successfully obtained.

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