Effects of melatonin on partial unilateral ureteral obstruction induced oxidative injury in rat kidney

This experimental study was designed to produce ischemia-reperfusion injury in rat kidney by performing partial unilateral ureteral obstruction [PUUO] and investigated the effects of melatonin on the levels of oxidative injury parameters. Twenty-four adult male rats were randomly divided into three groups as follows; control group [Group 1]; only nephrectomy and blood [5 ml] drawn from vena cava inferior, PUUO group [Group 2]; PUUO [10 days]+ipsilateral nephrectomy after recovery of PUUO+blood from vena cava inferior VCI, melatonin treated group [Group 3]; PUUO [10 days]+melatonin [1/2 hr before release, 50 mg/kg, ip]+ipsilateral nephrectomy after recovery of PUUO+blood from VCI. The left ureter was embedded into the psoas muscle to create PUUO. After 10 days, PUUO was recovered and ipsilateral nephrectomies were performed for biochemical analysis of superoxide dismutase [SOD], catalase [CAT], malondialdehyde [MDA], glutathione peroxidase [GSH-Px], and protein carbonyl [PC] in the tissues and blood was drawn from inferior vena cava to study the same parameters in systemic circulation. The results were compared statistically. The blood levels of MDA, NO, and PC were increased in the PUUO group in comparison to the sham-operated group [P<0.05]. Melatonin treatment reduced MDA, NO, and PC levels in blood after PUUO recovery, but statistically significance consisted only for MDA and NO [P<0.05]. The antioxidant enzyme activities [SOD, GSH-Px] were increased in the PUUO group [P<0.05]. Melatonin treatment reduced SOD and GSH-Px activities in comparison with the sham-operated control group [P<0.05]. Similarly, renal tissue levels of MDA, NO, and PC were increased in the PUUO group in comparison with the sham-operated group [P<0.05]. Melatonin treatment ameliorated MDA, NO, and PC levels in renal tissue after PUUO recovery only MDA was statistically significant [P<0.05]. Antioxidant enzyme activities [SOD, CAT, and GSH-Px] were increased in the PUUO group. Melatonin treatment caused reduction in SOD, CAT, and GSH-Px activities in comparison to the sham-operated control group [P<0.05]. The results of this study showed that experimentally induced PUUO caused oxidative stress in rat kidney and melatonin treatment reduced oxidative stress and therefore may have a preventive effect on PUUO induced oxidative kidney damage in rats

Levels of oxidative stress parameters and the protective effects of melatonin in psychosis model rat testis / 亚洲男科学杂志(英文版)

<p><b>AIM</b>To evaluate the effects of melatonin on antioxidant enzyme levels and histopathologic changes in dizocilpine (MK-801)-induced psychosis model rat testis.</p><p><b>METHODS</b>A total of 24 adult male Wistar-Albino rats were divided into three groups with 8 in each. Group I was used as control. Rats in Group II were injected with MK-801 (0.5 mg/kg body weight i.p. for 5 days). In addition to MK-801, melatonin (50 mg/kg body weight i.p. once a day for 5 days) was injected into the rats in Group III. The testes were harvested bilaterally for biochemical and histopathological examinations. Antioxidant enzyme activities, malondialdehyde, protein carbonyl and nitric oxide (NO) levels in testicular tissues were analyzed using spectrophotometric analysis methods. Histopathological examinations of the testes were also performed.</p><p><b>RESULTS</b>MK-801 induced testicular damage, which resulted in significant oxidative stress (OS) by increasing the levels of antioxidant enzymes. The malondialdehyde, protein carbonyl and NO levels were increased in testicular tissues of rats. Treatment with melatonin led to significant decrease in oxidative injury. Administration of melatonin also reduced the detrimental histopathologic effects caused by MK-801.</p><p><b>CONCLUSION</b>The results of the present study showed that MK-801 cause OS in testicular tissues of rats and treatment with melatonin can reduce the harmful effects of MK-801.</p>