Biological dose estimation by cytokinesis-block micronucleus assay on the patients exposed to accidental radiation / 解放军医学杂志

Objective A biological dose estimation by cytokinesis-block micronucleus(CBMN)assay was performed on 2 patients exposed to accidental radiation.Method Micronucleus assay and dose estimation by CBMN in peripheral blood and bone marrow were performed in 2 patients(A and B)suffering from radiation sickness.Results No binucleated lymphocytes were observed in peripheral blood of 2 patients.The results of bone marrow examination showed that only one binucleated cell was discovered in patient A.The rough estimation of radiation dose of A was above 20Gy according to the degree of reduction of binucleated cell in our previous study.The micronucleus frequency was 2.42 per cell and the dose estimation was 8.7(8.0-9.4)Gy for patient B.These results were approximately in accordance with that of dose estimations by physical method,chromosome aberration analysis,ESR method,and clinical manifestations.Conclusion MN assay is simple,quick and accurate.It is a reliable biodosimetry in addition to chromosome aberration analysis.

Dynamic observations on the cytogenetics of the exposed patients in Shandong “10?21” radiation accident / 解放军医学杂志

Objective To evaluate the engraftment state after allogeneic peripheral blood stem cell transplantation(Allo-PBSCT)in 2 exposed patients in Shandong “10?21” radiation accident.Methods The dynamic cytogenetic analysis was performed by chromosome aberration(CA)analysis,R-band karyotyping analysis and micronucleus(MN)assay of peripheral blood and/or bone marrow samples of 2 patients(A and B).Results The results showed that the transplantation was successful(complete chimerism),and the engraftment state was stable.No difference was observed between the results of cytogenetic analysis from peripheral blood samples and bone marrow samples.These results were in fairly good accordance with those results by short tandem repeats-polymerase chain reaction(STR-PCR)and clinical manifestations.Conclusions The results suggested that the dynamic observations of dicentric and ring aberrations and MN can be used as an indication to estimate the engraftment state after Allo-PBSCT,but only for the patient with acute radiation sickness.Moreover,the cytogenetic analysis performed on bone marrow samples is better than that on peripheral blood samples when the lymphocytes were reduced sharply in peripheral blood.