Optimization of ultrasound - assisted extraction of phenolic compounds and flavonoids from Hortia oreadica leaf / Optimización de la extracción asistida por ultrasonido de compuestos fenólicos y flavonoides de la hoja de Hortia oreadica

Hortia oreadica is indiscriminated used by people from Cerrado. However, vegetable raw material quality is decisive in obtaining inter mediate and final products. So, this study aimed to establish quality parameters of H. oreadica . For this, we performed the phytochemical screening of H. oreadica leaf and identified the best extractive conditions for phenolic compounds and flavonoids usin g factorial experimental design, varying the alcoholic strength, extraction temperature, and solid/liquid ratio in the ultrasound - assisted extraction method. The optimum extraction condition for phenolic compounds and flavonoids was 60% alcoholic strength, 40°C temperature, and a solid/liquid ratio of 8 mg/m L . Under this setting, the phenolic and flavonoid contents were 0.171 ± 0.002 mg/m L (predicted value = 0.165) and 0.087 ± 0.002 mg/m L (predicted value = 0.084), respectively. The optimized extraction par ameters could be upscaled to develop pharmaceutical drugs or nutraceutical products from this non - traditional plant species using an eco - friendly approach.

Hortia oreadica es utilizada indiscriminadamente por la gente del Cerrado. Sin embargo, la calidad de la materia prima vegetal es determinante en la obtención de productos intermedios y finales. Por lo tanto, este estudio tuvo como objetivo establecer parámetros de calidad de H. oreadica . Para ello, realizamos el tamizaje fitoquímico de la hoja de H. oreadica e identificamos las mejores condiciones extractivas para compuestos fenólicos y flavonoides mediante un diseño experimental factorial, variando el grado alcohólico, la temperatura de extracción y la relación sólido/líquido en el método de extracción asistido por ultrasonido. La condición óptima de extracción para compuestos fenólicos y flavonoides fue de 60% de grado alcohólico, 40°C de t emperatura y una relación sólido/líquido de 8 mg/m L . Bajo esta configuración, los contenidos de fenoles y flavonoides fueron 0,171 ± 0,002 mg/m L (valor previsto = 0,165) y 0,087 ± 0,002 mg/m L (valor previsto = 0,084), respectivamente. Los parámetros de ext racción optimizados podrían ampliarse para desarrollar fármacos o productos nutracéuticos a partir de esta especie de planta no tradicional uti lizando un enfoque ecológico .

An eco-friendly method for extraction and quantification of flavonoids in Dysphania ambrosioides

ABSTRACT Dysphania ambrosioides (L.) Mosyakin and Clemants (Syn: Chenopodium ambrosioides L.), Amaranthaceae, is a plant with antibacterial, antifungal, antioxidant, antiparasitic and antitumor properties that is commonly used in Brazilian folk medicine. In this work we performed the optimization of ultrasound-assisted extraction of flavonoids in the aerial parts of D. ambrosioides. The flavonoid concentrations, as rutin equivalents, were quantified with the aid of a validated spectrophotometric method. The Box-Behnken (33) design with response surface methodology, for the independent variables, extraction time, temperature, and ethanol content, were used for the optimization of ultrasound-assisted extraction. The analytical method was selective, linear, without matrix interference, accurate, precise and robust. The best conditions for the ultrasound-assisted extraction of flavonoids were: time of 60 min, temperature of 57 °C and ethanol content of 57% (w/w). The methods of extracting and quantifying flavonoids developed in the present study have provided be eco-friendly, simple, and useful to determine the flavonoid content, expressed as rutin equivalents, in the aerial parts of D. ambrosioides.