RESUMO
Recently, it has been shown that immunological methods can be used for the diagnosis of malaria other than sero-epidemiology. A study has been done to investigate optimum binding capacity of antigen-antibody (Ag-Ab) at different serum dilutions. For validating antigen-antibody (Ag-Ab) reaction at 1:100, 1:1000 and 1:1000 serum dilutions, have been tested in two different laboratories to establish validation of the ELISA method. Inter laboratory test on synthetic peptide (RI) ELISA was found comparable and meaningful for assessing malaria transmission in defined locality at 1:100 dilution. Results also showed that 1:1000 serum dilution can be useful for diagnostic purpose.
Assuntos
Sítios de Ligação de Anticorpos/imunologia , Coleta de Amostras Sanguíneas/métodos , Método Duplo-Cego , Ensaio de Imunoadsorção Enzimática/economia , Humanos , Índia/epidemiologia , Malária Falciparum/sangue , Proteínas de Protozoários/diagnóstico , Sensibilidade e Especificidade , Estudos SoroepidemiológicosRESUMO
Image contrast in the tilted beam mode of dark field (DF) imaging with a conventional transmission electron microscope has been estimated theoretically for two well-known biological macromolecules viz. lambda-phage DNA and BSA precursor protein. The DF contrast for electron accelerating voltage 0.1 to 3.0 MV has been compared with the corresponding bright field (BF) contrast. The DF contrast is seen to be so overwhelmingly greater than the BF contrast, for a wide range of accelerating voltage (phi) and aperture (alpha), that even an unstained bio-macromolecule should become visible under high voltage DF imaging.