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1.
Indian J Exp Biol ; 2000 Aug; 38(8): 791-6
Artigo em Inglês | IMSEAR | ID: sea-56765

RESUMO

Brugia malayi microfilarial excretory-secretory (mf ES) and phosphate buffer saline soluble (mf S) antigens were fractionated by fast protein liquid chromatography (FPLC) on superdex 200 HR 10/30 gel filtration column. The active antigen fractions were identified and explored in comparison with whole mf ES and mf S antigens to detect filarial IgG antibodies in different groups viz microfilaraemics, acute, chronic and occult filarial cases of Wuchereria bancrofti infection and endemic and non-endemic normals. One of the fractions of mf ES antigen (ESF-6) and two fractions of mf S antigen (SF-2 & 3) were identified to be useful to detect filarial antibodies. A pooled preparation of these antigen fractions gave a sensitivity of 86.6% (for microfilaraemic cases) and a specificity of 95% to detect filarial IgG antibodies by indirect ELISA. The pooled FPLC purified mf antigens also showed 55-88% of cases of different grades of clinical filariasis and 65% of tropical pulmonary eosinophilia cases as positive for filarial antibodies. The pooled FPLC purified B. malayi mf antigens with higher specificity are preferable to whole mf ES and mf S antigens to detect active filarial infection in microfilaraemia and as well in different clinical entities of bancroftian filariasis.


Assuntos
Animais , Antígenos de Helmintos/sangue , Brugia Malayi/imunologia , Cromatografia em Gel , Cromatografia Líquida , Filariose/sangue , Sensibilidade e Especificidade
2.
J Indian Med Assoc ; 1977 Jul; 69(1): 16-7
Artigo em Inglês | IMSEAR | ID: sea-103296
4.
Indian J Med Sci ; 1971 Feb; 25(2): 95-7
Artigo em Inglês | IMSEAR | ID: sea-69319
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