Detection of bacterial growth in blood components using oxygen consumption as a surrogate marker in a tertiary oncology setup.

Microbiological contamination of blood and blood products is a well-recognised transfusion risk. This study was performed in the blood bank of our oncology centre, with an objective to detect bacterial contamination in our blood products using oxygen consumption as a surrogate marker [Pall Enhanced Bacterial Detection System (eBDS)]. Results revealed that the percentages of failed units were 1.16% for random donor platelets (RDP), 0.81% for single donor platelets (SDP) and 2.94% for packed red blood cells (PRBCs), of which one RDP and one SDP grew coagulase-negative staphylococcus, while one PRBC culture grew Gram-positive bacilli.

Knowledge, attitudes and behaviours ofadolescents in relation to STIs, pregnancy,contraceptive utilization and substance abuse inthe Mhlakulo region, Eastern Cape

Sexually transmitted infections (STIs) and HIV/AIDS are a major problem in South Africa. This; coupled with a high incidence of teenage pregnancy; alcohol and drug abuse; is of grave concern; especially its impact among the young (15-24 years) and in economically poor; rural populations. This study aimed to assess the youths' knowledge; attitudes and behaviours regarding STIs; teenage pregnancy; contraception and substance abuse.Methodology: This is an interview-based; descriptive study. The sample design employed a stratified sample (using schools as strata) of young people aged 15 to 24 years in three schools in the Mhlakulo region; Eastern Cape province. From each school; a sample of learners from grades 10 to 12 was selected randomly. Questionnaires covering relevant parameters were used to interview the learners; after which the data were assimilated and analysed.Results: A total of 150 learners were surveyed (86 females and 64 males). In total; 56of them knew about STIs. About 88of the participants learned about STIs from health care workers/nurses/doctors/clinics; the media; educators; the school and friends. Most preferred to communicate to friends (38.67) and siblings (28); only 15communicated with parents. Among the sexually active; 54reported the use of condoms; of these only 62used them consistently. Of the participants; 7.33had more than five sexual partners. Of the young women; 12.8reported to have fallen pregnant with one-sixth of them wanting to become pregnant. Thirty per cent of those pregnant had to quit school; but did return subsequently. Common contraceptives used were condoms (54) and pills (58). Twenty-two per cent of the youths admitted to the use of recreational drugs at some time; most of these were related to alcohol (19.33). A small fraction (1.33) used dagga (cannabis).Conclusion: There is lack of knowledge of STIs and their prevention and condom and contraceptive use among young people of this community. Sexual promiscuity and teenage pregnancy in the group is a cause for concern. Substance abuse is another important problem that requires urgent attention

Bacteriological Profile and Antibiogram of Aerobic Burn Wound Isolates in Mthatha; Eastern Cape; South Africa

Burn wound colonisation and infection is not only associated with delayed wound healing and scar formation; but may also lead to sepsisrelated mortality. A wide variety of microorganisms; like staphylococcus aureus; Pseudomonas aeruginosa; and Enterobacteriaceae-like Klebsiella pneumoniae and Escherichia coli; are involved. Resistance is generally increasing; with reports of multidrug-and pan-resistant isolates. This study was conducted to determine the common aerobic bacterial isolates in our setting and describe their antimicrobial susceptibility. This retrospective; descriptive study was carried out on 243 patients; from whom 312 burn wound specimens were received by the Nelson Mandela Academic Hospital microbiology laboratory of the National Health Laboratory Service; Mthatha. All samples were processed according to standard laboratory protocols; isolates were tabulated according to age and gender of the patients; and their percentage susceptibilities to relevant antibacterials were computed. A total of 229 patient specimens showed growth on culture. The total number of isolates was 629; out of which 269 were Gram-positive cocci and 360 were Gram-negative bacilli. The commonest organism was S. aureus (27.7); followed by K. pneumoniae (13.4); Proteus mirabilis (12.4); Group D streptococcus (9.4); P. aeruginosa (8.9) and E. coli (6.2). A generally high level of resistance was observed in many organisms. Methicillinresistant S. aureus accounted for 57.5of the S. aureus. Resistance among the Gram-negative bacilli was; in general; least to imipenem; amikacin and ciprofloxacin. The common organisms causing burn wound infections in our setting include staphylococci; Klebsiella; Proteus and Pseudomonas and there is a high level of resistance against commonly used antimicrobials. Regular surveillance of burn wound organisms and their antimicrobial resistance patterns will help in determining empirical antibiotic therapy for subsequent related septic events

Prevalence of goitre among school children in Belgaum District.

Objective. A school survey was conducted to estimate the prevalence of goitre among schoolchildren in Belgaum district. Methods. A cross-sectional study was conducted in primary, middle and high schools of villages selected. All the children of the selected schools were examined for the presence of goitre and the salt samples obtained from their homes were tested for iodine content. Results. Overall prevalence of goitre was 16.7%. Prevalence of palpable goitre was 16.4 % and visible goitre was very low (0.3%). Higher prevalence was found among females (21.1%) when compared to that of male children (12.8 %). Prevalence of goitre increased significantly with advancement of age until 16-yr. 72.1 % children were consuming rock salt and only 27.9 % were consuming powdered salt at their homes. Estimation of Iodine content in the salt samples showed that 68.7 % of the sample had inadequate iodine content. Prevalence of goitre was significantly high among children who consumed rock salt (16.2%) as compared to those who used powdered salt (11%). Conclusion. Strict implementation of salt codization and marketing in rural area is desired. Also health education programme be showed.

Neonatal melioidosis: A case report from India.

Melioidosis, caused by Burkholderia pseudomallei , is an infectious disease of major public health importance in Southeast Asia and Australia. We report, for the first time from the Indian subcontinent, a case of melioidosis in a neonate, its clinical presentation, microbiological diagnosis, possible mode of transmission and outcome. A pre-term female baby developed respiratory distress soon after birth. The child was febrile, had tachypnea, grunting, normal heart rate with a low pulse volume and poor peripheral perfusion. Chest X-ray revealed right-sided bronchopneumonia. B. pseudomallei was isolated from the blood culture of the neonate collected aseptically. The neonate was successfully treated with meropenem.

Correlation between Different PBL Assessment Components and the Final Mark for MB ChB III at a Rural South African University

Problem-based learning (PBL) is now an accepted component of many medical school programmes worldwide. Our university also follows the PBL `SPICES' model for MB ChB III. The assessment modalities used are the modified essay questions (MEQ); objective structured practical examination (OSPE); individualised process assessment (IPA) and tutorial continuous assessment (TUT). This study was done to compare the students' performances in individual assessment components with the final mark to determine the correlation between these parameters. Materials and methods. The study was retrospective; descriptive and analytical; based on the integrated marks of all the MB ChB III students at Walter Sisulu University (WSU) in 2007. Assessment marks were stratified according to blocks and different types of assessment (MEQ; TUT; OSPE; IPA). Regression analysis was used to compute and scrutinise these vis-a-vis their correspondence with the final marks for each block.Results. Three hundred and seventy-nine block assessment marks of 96 students from 4 blocks of MB ChB III were analysed and the correlation between the assessment components and final mark were compared. Regression analysis showed good correlation when analysing the assessment modality versus the final mark for the MEQs (r=0.93; 0.93; 0.94; 0.96); followed by OSPEs (r=0.71; 0.70; 0.76; 0.77) and IPAs (r=0.62; 0.51; 0.68; 0.77). However; correlation was not significant with the TUT. Conclusion. There was good correlation between the students' performance in the majority of assessment modalities and the final mark in the different blocks of the MB ChB III examination. There may be a need to make tutorial assessment methods more objective; partly by additional tutor training

Primary meningeal melanocytoma.

Meningeal melanocytoma is an uncommon benign pigmented primary lesion that has to be distinguished as a pathological entity separate from other benign melanin containing tumours as well as its overtly malignant counterpart, melanoma. In the present study, a 58 year old lady presented with bilateral sensory and motor symptoms in the lower limbs. The MRI scan showed a dumb-bell shaped lesion in the spinal canal at 6th cervical vertebra to 1st thoracic vertebra (C6-T1) level mimicking a schwannoma and histologically proved to be melanocytoma.

Influence of parental literacy and socio-economic status on infant mortality.

The influence of parental literacy and socio-economic status (SES) on infant mortality rate (IMR) was studied. This report is only a part of much larger community-based prospective study conducted to determine the factors influencing infant mortality in Dakshina Kannada district of Karnataka, by the Department of Community Medicine, Kasturba Medical College, Manipal, with the financial assistance form IDRC, Canada. A total of 12,857 livebirths during the period of October 1, 1991, to September 30, 1992, where registered and followed up for one year. There were 391 infant deaths which gives an IMR of 30.4 per 1,000 livebirths. Univariate analysis and subsequent stepwise multiple logistic regression analysis revealed that, literacy and SES have a very significant role in reducing IMR.

Nerve injuries due to obstetric trauma.

The incidence of nerve injuries among 32,637 deliveries over a period of ten years was 1.81/1000. Brachial plexus injury (1/1000) and facial nerve injury (0.74/1000) accounted for 98% of nerve injuries. Both the right and left side were involved equally. Bilateral nerve injury was not seen. Lack of antenatal care, macrosomia, abnormal presentations, and operative vaginal deliveries significantly increased the risk of nerve injuries. These babies had significantly higher incidence of meconium stained liquor and intrapartum asphyxia. Parity of the mother, gestational age and sex of the baby did not have significant role in the causation of nerve injuries. Injuries to brachial plexus and facial nerve were seen even in babies born by caesarean section, when it was performed for obstructed labour caused by cephalo-pelvic disproportion and abnormal presentations. Three babies with injuries expired and forty-three could be followed up for varying periods. None of the babies had residual defects. Detection of cephalopelvic disproportion and abnormal lie in the third trimester and their appropriate management would decrease the incidence of obstetric palsies to a significant extent.

A case of spindle cell haemangioendothelioma.

Spindle cell haemangioendothelioma (SCH) is a low grade, slowly progressive angiosarcoma of multifocal nature with local recurrences being common. It is histologically marked by cavernous blood spaces and a spindle cell stroma. Its importance lies in recognising its relatively benign nature and differentiating it from the ominous Kaposi's sarcoma. Our case reiterates the characteristics of this recently described disease, probably the first reported from India.

Mosquito repellent activity of oils from Vitex negundo Linn. leaves.

Oil obtained from stream distillate of V. negundo leaves was fractionated by column chromatography. Mosquito repellence activity, as evaluated against Aedes aegypti was mainly confined to the most polar fractions. The protection period against mosquito bites by polar fractions ranged between 1-3 h. However, the mean protection period values of these fractions did not show significant increase in the subsequent subfractions.