RESUMO
Bacterial meningitis caused by Neisseria meningitidis which causes human brain meninges damage, is generally diagnosed from patient cerebrospinal fluid through microscopy, immunological assays, biochemical test, PCR, microarray and biosensors. However, these methods are expensive, time-consuming or non-confirmatory due to certain limitations. A quick PCR based method was developed for detection of bacterial meningitis caused by N. meningitidis using specific primers based on amplification of virulence nspA (Neisseria surface protein A) gene partial sequence (202 bp). The nspA gene amplicon could be used as a genetic marker for minimum detection of 10 ng genomic DNA (G-DNA) of N. meningitidis with high sensitivity only in 80 min, which is least time reported for the confirmation of the disease. However, the lower detection limit was found as low as 1.0 ng G-DNA, but with less sensitivity. The cross-reactivity of the genetic marker was also studied with other possible pathogens. A comparison with the presently available detection methods and our method was also done using patient samples.
Assuntos
Proteínas da Membrana Bacteriana Externa/genética , Sequência de Bases , Marcadores Genéticos/genética , Humanos , Infecções Meningocócicas/diagnóstico , Infecções Meningocócicas/microbiologia , Dados de Sequência Molecular , Neisseria meningitidis/genética , Neisseria meningitidis/isolamento & purificação , Reação em Cadeia da Polimerase , Sensibilidade e EspecificidadeRESUMO
Topical use of timolol eye drops in the management of glaucoma is associated with various systemic side effects including alteration of lipid profile with increase in cardiovascular risk factors. Change over to timolol gel altered results in normalisation of altered lipid profile with decrease in cardiovascular risk factors. The decrease seen is attributable to lack of systemic absorption of timolol gel.