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Objective To study the impact on the proliferation and cytotoxicity of donor T cells during mobilization with rhG - CSF. Methods The peripheral blood mononuclear cells (PBMNC) were collected from 20 healthy donors before mobilization and on the fifth day of mobilization with rhG - CSF. After the PBMNC was activated with 500 ng/ml of CD3 monoclonal antibody and 500 ?g/ml of rhIL -2 for 96 hours,the activated T cells were collected for testing proliferation, cytotoxicity. Fas expression, perforin and Fas ligand (FasL) mRNA expression,and IFN -? concentration in the culture medium of the activated T cells, were determined by radioimmunoassay. Results The proliferation activity and cytotoxicity of T cells activated with CD3 monoclonal antibody and rhIL - 2 were reduced markedly after mobilization with rhG - CSF(P0.05). The activated T cells expressed perforin mRNA and didn' t express FasL mRNA both before and after mobilization with rhG - CSF. The concentration of IFN -? in the culture medium of the activated T cells decreased significantly after mobilization with rhG - CSF(P
RESUMO
Objective To investigate the blood cell separator collection of peripheral blood stem cell after using recombinant human granulocyte colony - stimulating factor (rhG - CSF) to mobilize the normal donor and patients with hematologic malignancies, through morphological observation of lymphocyte ratios and PBSC vitality of mononuclear cells. Methods Peripheral blood mononuclear cells (MNC)were collected from 15 cases of healthy donors and 14 patients of hematologic malignancies with rhG - CSF mobilization dose with blood cell separators. The number of nucleated cells, classification of nucleated cells, MNC count and cell viability were observed. Results ①The total number of nucleated cells in normal donor group was(4.31?1.41)?10~8 / kg,and hematologic malignancies group was (6.21?4.37)?10~8/kg. ②The number of nucleated cells of PBSC in hematologic malignancies group was higher than those of the donor group (P0.05).④MNC number was (96.7? 5.1) % in normal donor group counts, and in which lymphocytes was (65.9?9.4)% , myeloid (16.3?8.7)% , mononuclear cells (16.5?4.0)% ,respectively,MNC number was (92.7?15.6)% in hematologic malignancies group, and in which lymphocytes was (55.3?16.7)% , myeloid (24.3?16.5) % , mononuclear cells (14.9?9.1)%, respectively. ⑤Cell viability in normal donor group before and after cryopreservation were (91.5?4.3)%, (67.4?9.1) % , in hematologic malignancies grou were (82.9?11.1) % , (56.5?20.1) % , respectively. Cell viability was significantly different (P