A Comparative Study of Lipid Profile in Type I and Type II Diabetes

Background:Prevalence of D.M. has been steadily increasing in urban as well as rural areas in India & it will be one of the major cause of death in India in 21st century. Pattern of dyslipidemia is found to be different in Type I & Type II diabetes mellitus (DM). Aims and objectives: 1) Estimation of lipid profile in Type I and Type II DM. 2) Comparison of Lipid Profile in Type I and Type II DM. Material and methods: 50 patients of each already diagnosed Type I and Type II DM patients visiting OPD. 50 age and sex matched healthy controls. FPG (Fasting Plasma Glucose) and lipid profile were studied in the groups. Statistics: Students‘t’ test was used. Results: FPG and TC, TG, LDL-C, VLDL-C were significantly high in both Type I and Type II DM compared to the controls. While HDL-C was significantly lower in diabetics compared to the controls. Among the diabetics all the parameters were significantly high in Type IIDM compared to Type I DM exceot HDL-C in which no significant difference was found. Conclusion: Glycaemic control is poorer in Type II DM compared to Type I DM. Also, dyslipidaemia is more prominent in Type II DM than in Type I DM.

Study of Lipid Profile and Glycated Hemoglobin in Diabetes Mellitus.

Background: Pattern of dyslipidemia is found to be different in type-1 & type-2 diabetes mellitus(DM). Dyslipidemic pattern and its correlation with glycated hemoglobin in both types of diabetes mellitus has been well studied and might be informative to the diagnostic,therapeutic & prognostic aspects of diabetes mellitus. AIM: To study the lipid profile by using direct method for High Density Lipoprotein-Cholesterol (HDLC) & Low Density Lipoprotein-Cholesterol(LDL-C) estimation, and glycated hemoglobin(GHb) in type-1DM & type-2 DM. Material and Methods: fifty each already diagnosed patients of type-1 and type-2 diabetes mellitus along with fifty control were studied for lipid profile using direct method for Low Density Lipoprotein-Cholesterol (LDL-C) & High Density Lipoprotein-Cholesterol (HDLC) estimation and glycated hemoglobin status. Statistical Analysis: we used student t-test and Pearson’s correlation coefficient to find the statistical significance. Result: serum concentration of glycated hemoglobin and all the parameters of lipid profile except HDL-C were increased while HDL-C concentration decreased in both the types of DM as compared to that of control. Conclusion: dyslipidemia is more prominent in type-2 DM than that in type-1 DM . Glycemic control is poorer & its correlation with lipid profile is stronger in type-2 DM as compared to type-1 DM.

Isolation, purification and partial characterisation of prealbumin from cerebrospinal fluid.

Prealbumin from human cerebrospinal fluid was purified using a combination of ammonium sulphate precipitation, phenol precipitation, Polyacrylamide disc gel electrophoresis and gel filtration on Sephadex G-100. The homogeneity of the purified protein was established by Polyacrylamide gel electrophoresis and Immunoelectrophoresis. On the basis of its molecular weight (55,000), amino acid composition, electrophoretic mobility and immunological cross-reactivity, the prealbumin from cerebrospinal fluid showed complete identity with serum prealbumin. The cerebrospinal fluid prealbumin levels in various neurological disorders may have a diagnostic significance.