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1.
Journal of Medical Biomechanics ; (6): E370-E376, 2014.
Artigo em Chinês | WPRIM | ID: wpr-804321

RESUMO

Objective To discuss the possibility for regeneration of knee joint with normal function under bionics biomechanical environment. Methods Seven normal rabbits with two metal frames respectively mounted on their femur and tibia of single (right) side leg by two threading pins of 1.5 mm diameter were used. Then the external artificial joints, containing two four-bar-linkage inside and outside of the knee to simulate motion trajectory of the joint in sagital plane were connected with the two preset fixed frames before osteotomy. The arthro-cartilage of the knee joint, crucial ligaments, semi-lunar fibro-cartilages, and partial under-cartilage-osseous intra articular capsule were cut off during osteotomy, with the patella, sesamoid bones remained intact. Insertion sites of muscle tendons were not invaded as far as possible, and capsules with ligaments outside were kept complete when the incision wound was closed by suture. The rabbits could move freely after osteotomy. The range of motion (ROM) of the knees in sagital plane and bony gap between the femur and tibia were measured by X-Ray films during the fracture healing after osteotomy. Results External artificial knee joints were successfully installed on right legs of 6 rabbits among the seven. The rabbits moved freely after osteotomy under the control of minimal invasive external artificial joint in bionics trajectory. The average angles between femoral shaft and tibial shaft at the 1st week after osteotomy were from (144.7±15.62)° in extremely flexed position to (44.2±25.77)° in extremely extended position, with ROM of (100.5±29.03)°. At the 12th week, the average angles were from (139.4 ± 12.92)° in extremely flexed position to (40.4±22.04)° in extremely extended position, with ROM of (99.0±23.39)°. No significant differences were displayed in flexed/extended position and ROM between the 1st and 12th week, with the bony gaps of the knees still existed but decreased significantly from (4.03±1.84) mm at the 1st week to (2.32±1.05) mm at the 12th week. In contrast, bony gaps of the opposite knees were not changed significantly, which were (1.27±0.22) mm on average. At the end of 16th week after osteotomy, the external artificial joints were removed. Newly born cartilage, with white color and smooth surface, were covered at lower end of the femur and upper end of the tibia. Typical trochlear surface appeared at the front side of regenerated cartilage corresponding to the posterior surface of the patella. And the regenerated fibro-bundle linkage similar with ligament, which started from bony structure of regenerated lower end of the femur and inserted into regenerated upper end of the tibia, was observed in each rabbit. At the 25th week, the average angles between femoral shaft and tibial shaft were from (148.3 ± 4.75)° in extremely flexed position to (48.30±17.57)° in extremely extended position, with ROM of (100.0±20.80)°. In the opposite (left) leg, the average angles between femoral shaft and tibial shaft were from (148.3±7.5)° in extremely flexed position to (21.6±9.09)° in extremely extended position, with ROM of (126.7±6.88)°, and the average bony gap of the knees after osteotomy was (1.4±0.59) mm, while that of the opposite (left) knees was (0.92±0.35) mm. Conclusions The external artificial joint with bionics trajectory could reserve the space for regeneration of rabbit knee joint by providing motion modeling environment, and proved the stress adaption during fracture healing. The present results indicated that regeneration of the knee joint after intra-capsular osteotomy in bionics biomechanical environment was possible.

2.
China Journal of Chinese Materia Medica ; (24): 1740-1746, 2013.
Artigo em Chinês | WPRIM | ID: wpr-294031

RESUMO

Eighteen lignans were isolated from an ethanol extract of Machilus robusta by a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20 and reversed-phase HPLC. Their structures were identified by spectroscopic data analysis as isolariciresinol-9'-O-beta-D-xylopyranoside (1), (+)-5'-methoxy-isolariciresinol-9'-O-beta-D-xylopyranoside(2), lyoniresinol-9'-O-beta-D-xylopyranoside(3), (+)-(8S, 8'S) -4, 4'-dihydroxy-3, 3', 5, 5'-tetramethoxylignan-9, 9'-diol 9-O-beta-D-xylopyranoside (ssioriside, 4), lyoniresinol (5), meso-dihydroguaiaretic acid (6), (+)-(8S, 8'R)-3', 4, 4'-trihydroxy-3'-methoxylignan (7), (8S, 8'R)-4'-hydroxy-3, 3', 4-trimethoxylignan (meso-monomethyl dihydroguaiaretic acid, 8), (+)-guaiacin (9), isoguaiacin (10), (-)-(7'R, 8R, 8'R)-4, 4'-dihydroxy-3, 3', 5-trimethoxy-2, 7'-cyclolignan (11), henricine B (12), (-)-(7S, 7'S, 8R, 8'R)-4, 4'-dihydroxy-3, 3', 5, 5'-tetramethoxy-7, 7'-epoxylignan-9, 9'-dio] (7S, 7'S, 8R, 8'R-icariol A2, 13), (+)-(7R, 8R, 7'E)-4-hydroxy-3, 5'-dimethoxy-7, 4'-epoxy-8, 3'-neolignan-7'-ene (licarin A, 14), nectandrin B (15), machilin-I (16), (-)-pinoresinol (17), and (-)-syringaresinol (18). All compounds were isolated from this plant for the first time. In the preliminary assay, compound 17 showed inhibitory activity against NO secretion of mouse peritoneal macrophages with an inhibition rate of 72.2% at 10 micromol x L(-1).


Assuntos
Lauraceae , Química , Lignanas , Química , Espectrometria de Massas , Estrutura Molecular , Extratos Vegetais , Química
3.
China Journal of Chinese Materia Medica ; (24): 1172-1182, 2013.
Artigo em Chinês | WPRIM | ID: wpr-350638

RESUMO

Thirty-three compounds were isolated from the root decoction of Isatis indigotica by using a combination of various chromatographic techniques including silica gel, macroporous adsorbent resin, Sephadex LH-20, and reversed-phase HPLC. Their structures were elucidated by spectroscopic data as (+)-dehydrovomifoliol (1), (S)-(+)-abscisic acid (2), vomifoliol (3), cyclo (L-Phe-L-Leu) (4), cyclo(L-Phe-L-Tyr) (5), cyclo(L-Tyr-L-Leu) (6), cyclo(L-Pro-L-Tyr) (7), evofolin B (8), (+)-syringaresinol (9), (-)-(7R,7'R,8S,8'S)-4,4'-dihydroxy-3-methoxy-7,9';7',9-diepoxy-lignan (10), (-)-medioresinol (11), (+) -(7R,7'R,8S,8'S) -neo-olivil (12), (-) -5-methoxyisolariciresinol (13), 1,3-dihydro-2H-indol-2-one (14), isalexin (15), dihydroneoascorbigen (16), indican (17), (-) -(S) -cyanomethyl-3-hydroxyoxindole (18), isoformononetein (19), calycosin (20), stigamast-5-ene-3beta-ol-7-one (21), acetovanillone (22), 3, 5-dimethoxy-4-hydroxyacetophenone (23), dihydroconiferyl alcohol (24), dihyroferulic acid (25), 3-hydroxy-1-(4-hydroxyphenyl) propan-1-one (26), beta-hydroxypropiovanillone (27), 4-aminobenzoic acid (28), 3-(4-hydroxyphenyl) propan-1-ol (29), 4-(2-hydroxyethyl) phenol (30), 2-methoxy-4-vinylphenol (31), pyrocatechol (32), and 4-pentenamide (33). These compounds were isolated from the root of I. indigotica for the first time. In preliminary in vitro assays, compound 19 showed activity against the influenza virus A/Hanfang/359/95 (H3N2), the herpes simplex virus 1 (HSV-1), and Coxsackie virus B3 (Cox-B3), with IC50 values of 2.06, 6.84, and 8.70 micromol x L(-1), respectively, but other compounds were in-active at a concentration of 1.0 x 10 x (-5) mol x L(-1).


Assuntos
Animais , Humanos , Linhagem Celular , Isatis , Química , Extratos Vegetais , Química , Farmacologia , Toxicidade , Raízes de Plantas , Química
4.
Journal of Biomedical Engineering ; (6): 716-720, 2010.
Artigo em Chinês | WPRIM | ID: wpr-230798

RESUMO

Cardiac contractility variability (CCV) is a new concept which is introduced in the research field of cardiac contractility in recent years, that is to say, there are some disparities between cardiac contractilities when heart contracts. The changing signals of cardiac contractility contain a plenty of information on the cardiovascular function and disorder. In order to collect and analyze the message, we could quantitatively evaluate the tonicity and equilibrium of cardiac sympathetic nerve and parasympathetic nerve, and the effects of bio-molecular mechanism on the cardiovascular activities. By analyzing CCV, we could further understand the background of human being's heritage characteristics, nerve types, the adjusting mechanism, the molecular biology, and the adjustment of cardiac automatic nerve. With the development of the computing techniques, the digital signal processing method and its application in medical field, this analysis has been progressing greatly. By now, the assessment of CCV, just like the analysis of heart rate variability, is mainly via time domain and frequency domain analysis. CCV is one of the latest research fields in human cardiac signals being scarcely reported in the field of sports medicine; however, its research progresses are of important value for cardiac physiology and pathology in sports medicine and rehabilitation medicine.


Assuntos
Humanos , Sistema Nervoso Autônomo , Fisiologia , Exercício Físico , Fisiologia , Contração Miocárdica , Fisiologia , Fonocardiografia , Métodos , Processamento de Sinais Assistido por Computador
5.
Chinese Journal of Plastic Surgery ; (6): 345-348, 2010.
Artigo em Chinês | WPRIM | ID: wpr-268679

RESUMO

<p><b>OBJECTIVE</b>To investigate the change of zygomatic and temporal soft tissue after coronal incision.</p><p><b>METHODS</b>A retrospective analysis was performed in 33 patients who received firm fixation for unilateral zygomatic comminuted fracture through semi-coronal incision. All the patients were followed up for more than one year. Craniofacial anthropometric measurement through 3D-CT reconstruction and facial profile was performed. The difference between the operated side and healthy side was analyzed.</p><p><b>RESULTS</b>At the temporal concave point, the soft tissue thickness at healthy side was (1.60 +/- 0. 97) mm more than that at operated side, showing a significant difference between them (P < 0.01). While the soft tissue thickness was not statistically different between two sides at zygion, malar prominence, zygomaxillare, and temporal convex point (P > 0.05).</p><p><b>CONCLUSIONS</b>The soft tissue atrophy may happen at temporal fat pad after semi-coronal incision, but not at zygomatic area. Intraoperative precise dissection and less stretch of soft tissue may be helpful to avoid the postoperative facial asymmetry.</p>


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Tecido Adiposo , Seguimentos , Fraturas Cominutivas , Cirurgia Geral , Período Pós-Operatório , Estudos Retrospectivos , Couro Cabeludo , Cirurgia Geral , Fraturas Zigomáticas , Cirurgia Geral
6.
China Journal of Orthopaedics and Traumatology ; (12): 194-195, 2008.
Artigo em Chinês | WPRIM | ID: wpr-323179

RESUMO

<p><b>OBJECTIVE</b>In order to get three dimensional measurements simply an plain X-ray photographs.</p><p><b>METHODS</b>The multi-layers of bone pieces were made by use of bone saw, and piled up to spiral ladder, which was used as a calibrating module for to living bone in clinics. The relationship between the cortical thickness and gray level of the X-ray photographs was obtained.</p><p><b>RESULTS</b>The correlation can be described with a cubic regression curve, and it can be simplified to a linear regression equation when cortical thickness is small than 3.8 mm.</p><p><b>CONCLUSION</b>It can be realized to get three dimensional measures of the bone on plain X-ray photographs, by use of the bony spiral ladder described here.</p>


Assuntos
Adulto , Humanos , Osso e Ossos , Química , Diagnóstico por Imagem , Radiografia , Raios X
7.
China Journal of Orthopaedics and Traumatology ; (12): 103-105, 2008.
Artigo em Chinês | WPRIM | ID: wpr-323147

RESUMO

<p><b>OBJECTIVE</b>To explore the mechanical property of intact long bone through three-point bending test of rabbit tibia according to mechanics of materials.</p><p><b>METHODS</b>Ten pairs of normal rabbits tibia were treated on three-point bending test with 80 mm supporting distance in order to corroborate the virtual loading test on bone model.</p><p><b>RESULTS</b>Bending stiffness and strength of 10 pairs of normal rabbit tibia were obtained. The maximum flexibility of the right and the left tibia was (2.737 +/- 0.262) mm and (2.739 +/- 0.233) mm respectively. The maximum load, which indicated the bending strength, of the right and the left tibia was (17.803 +/- 2.675) kg and (18.366 +/- 2.653) kg respectively. The area under the load-flexibility curve before fail of the right and the left tibia was (23.829 +/- 4.413) kg/mm and (24.725 +/- 4.101) kg x mm respectively. The slope of the load-flexibility curve which indicate the stiffness of the right and the left tibia was (7.545 +/- 1.310) kg/mm and (7.631 +/- 1.174) kg x mm respectively.</p><p><b>CONCLUSION</b>The discrepancy between different animal body is larger than conventionally required. It's suggested for performing three-point bending test of long bone of the body both sides in pair.</p>


Assuntos
Animais , Coelhos , Fenômenos Biomecânicos , Amplitude de Movimento Articular , Tíbia , Fisiologia , Suporte de Carga
8.
Acta Pharmaceutica Sinica ; (12): 959-963, 2007.
Artigo em Inglês | WPRIM | ID: wpr-268547

RESUMO

To study the chemical constituents of Ardisia punctata, compounds were isolated with a combination of multi-chromatography. Their structures were determined on the basis of spectral analysis and comparison to those of the known compounds. A 1,4-benzoquinone derivative and a alkylphenol were isolated from the petroleum ether extract of the roots of Ardisia punctata. Their structures were elucidated as 2-tridecyl-3-[(2-tridecyl-4-acetoxy-6-methoxy)-phenoxyl] -6-methoxy-1,4-benzoquinone (1) and 2-methoxy-4-hydroxy-6-tridecyl-phenyl acetate (2). The two compounds are both new.


Assuntos
Ardisia , Química , Benzoquinonas , Química , Estrutura Molecular , Fenilacetatos , Química , Raízes de Plantas , Química , Plantas Medicinais , Química
9.
Chinese Journal of Plastic Surgery ; (6): 385-388, 2007.
Artigo em Chinês | WPRIM | ID: wpr-314212

RESUMO

<p><b>OBJECTIVE</b>To investigate the feasibility, technique and significance of preserving the posterior branch of the great auricular nerve in parotid surgery.</p><p><b>METHODS</b>48 cases with parotid tumor who underwent regular parotid surgery with cosmetic incision were included. The sensory testing was carried out in upper auricle, lobule, infra-auricular, pre-auricular and post-auricular region preoperatively and at 10 days, 1 month, 6 months and 12 months after operation.</p><p><b>RESULTS</b>The posterior branch of the great auricular nerve was preserved in 35 out of 48 patients. There was no sensory loss in the upper auricle and post-auricular region. Early after operation, sensory disturbance was found in lobule, infra-auricular region, but significantly in pre-auricular region. The sensory disturbance was recovered slowly to nearly normal level at six months after operation. 13 cases with sacrifice of the nerve had a significantly higher sensory disturbance and a longer recovery time.</p><p><b>CONCLUSIONS</b>Preservation of the posterior branch of the great auricular nerve during parotid surgery is technically feasible and can reduce the postoperative sensory disturbance and possible permanent sensory loss. It further helps to improve the quality of life early after operation.</p>


Assuntos
Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Orelha Externa , Glândula Parótida , Cirurgia Geral , Neoplasias Parotídeas , Cirurgia Geral , Complicações Pós-Operatórias
10.
Acta Pharmaceutica Sinica ; (12): 830-834, 2006.
Artigo em Inglês | WPRIM | ID: wpr-294930

RESUMO

<p><b>AIM</b>To study the chemical constituents of Ardisia punctata.</p><p><b>METHODS</b>Compounds were separated with a combination of multi-chromatography. Their chemical structures were determined on the basis of spectral analysis and single crystal X-ray diffraction.</p><p><b>RESULTS</b>Three compounds were isolated from chloroform extract of the roots of Ardisia punctata. Their structures were elucidated as 2-tridecyl-3-[(2-tridecyl-3-acetoxy-4-methoxy-6-hydroxy) -phenyl]-6-methoxy-1, 4-benzoquinone (1), 2-tridecyl-3-[(2-tridecyl-4,6-dihydroxy) -phenyl]-6-methoxy-1,4-benzoquinone (2) and 2-tridecyl-3-[(2-pentadecyl-4,6-dihydroxyl) -phenyl]-6-methoxy-,4-benzoquinone (3).</p><p><b>CONCLUSION</b>The three compounds are new 1,4-benzoquinone derivatives.</p>


Assuntos
Ardisia , Química , Benzoquinonas , Química , Conformação Molecular , Estrutura Molecular , Fenóis , Química , Raízes de Plantas , Química , Plantas Medicinais , Química , Resorcinóis , Química
11.
China Journal of Chinese Materia Medica ; (24): 131-133, 2006.
Artigo em Chinês | WPRIM | ID: wpr-350991

RESUMO

<p><b>OBJECTIVE</b>To study the chemical constituents from the roots of Ficus hirta.</p><p><b>METHOD</b>Compounds were isolated by chromatographic techniques on silica gel and HP-20 resin columns. Their structures were elucidated by chemical and spectroscopic methods.</p><p><b>RESULT</b>Ten compounds were identified as beta-sitosterol (1), stigmasterol (2), psoralene (3), 3beta-hydroxy-stigmast-5-en-7-one (4), 5-hydroxy-4', 6, 7, 8-tetramethoxy flavone (5), 4', 5, 6, 7, 8-pentamethoxy flavone (6), 4', 5, 7-trihydroxy-flavone (7), 3beta-acetoxy-beta-amyrin (8), 3beta-acetoxy-alpha-amyrin (9) and hesperidin (10).</p><p><b>CONCLUSION</b>The compounds 4, 5, 6 were obtained from this genus for the first time, and all the compounds were obtained from this plant for the first time.</p>


Assuntos
Ficus , Química , Ficusina , Química , Flavonas , Química , Ácido Oleanólico , Química , Raízes de Plantas , Química , Plantas Medicinais , Química , Sitosteroides , Química , Estigmasterol , Química
12.
China Journal of Chinese Materia Medica ; (24): 562-565, 2006.
Artigo em Chinês | WPRIM | ID: wpr-356768

RESUMO

<p><b>OBJECTIVE</b>To study the chemical constituents from the roots of Ardisia punctata.</p><p><b>METHOD</b>Compounds were isolated by chromatographic techniques on silica gel and Rp-HPLC column. Their structures were elucidated by chemical and spectroscopic methods.</p><p><b>RESULT</b>Twelve compounds were identified as 3-hydroxy-5-tridecyl-methyl phenyl ether (1), 5-pentadecyl-1, 3-benzenediol (2), 2-methoxy-6-tridecyl-1, 4-benzoquinone (3), 2-methoxy-6-pentadecyl-1, 4-benzoquinone (4), glutinol (5), ardisicrenoside A (6), ardisiacrispin B (7), 24-ethyl-5a-cholesta-7, 22(E)-dien-3-one (8), 24-ethyl-5alpha-cholesta-7, 22(E)-dien-3beta-ol (9), daucosterol (10), vanillin acid (11), tetratriacontanoic acid (12).</p><p><b>CONCLUSION</b>All the compounds were obtained from this plant for the first time.</p>


Assuntos
Ardisia , Química , Benzoquinonas , Química , Ácido Oleanólico , Química , Raízes de Plantas , Química , Plantas Medicinais , Química , Saponinas , Química , Triterpenos , Química
13.
Chinese Journal of Sports Medicine ; (6)1982.
Artigo em Chinês | WPRIM | ID: wpr-584575

RESUMO

Objective To investigate the effects of different exercise loads on the myocardial expression of vascular endothelial growth factor (VEGF) in rats. Methods 54 male SD rats were randomly divided into control group(C), moderate-load training group(MT), high-load training group(HT). Both MT and HT group were subdivided into three groups:swimming training for 1 week(n=6),3 weeks (n=6)and 5 weeks(n=6). The general conditions during the training were observed. The expression of VEGF and histological changes were investigated. Results The results showed that the expression of VEGF in MT group increased (P

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