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1.
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 148-152, 2009.
Artigo em Chinês | WPRIM | ID: wpr-337529

RESUMO

<p><b>OBJECTIVE</b>To observe the in vitro anticancer effect of Nispex, the total flavonoids extract from Scurrula parasitic L.</p><p><b>METHODS</b>The cell proliferation inhibitory effects of Nispex on various kinds of tumor cells or non-tumor cells in human and rats were detected with MTT assay and colony forming assay respectively, the cell apoptosis induced by Nispex was detected by AO/EB fluorescence staining, TUNEL assay and AnnexinV-FITC/PI flow cytometry.</p><p><b>RESULTS</b>Nispex could significantly inhibit human cancer cell proliferation and induce human cancer cell apoptosis, especially to the proliferative cell group, but its inhibition on human non-tumor cell was insignificant, and showed no effect on murine cancer cells in the tested scope.</p><p><b>CONCLUSION</b>Nispex is a nature plant extract which shows good selectivity for killing human cancer cell.</p>


Assuntos
Animais , Humanos , Ratos , Antineoplásicos Fitogênicos , Farmacologia , Apoptose , Proliferação de Células , Medicamentos de Ervas Chinesas , Química , Flavonoides , Farmacologia , Loranthaceae , Química , Neoplasias , Patologia , Especificidade da Espécie , Células Tumorais Cultivadas
2.
China Journal of Chinese Materia Medica ; (24): 427-432, 2008.
Artigo em Chinês | WPRIM | ID: wpr-284466

RESUMO

<p><b>OBJECTIVE</b>To compare the anticancer effects of flavonoids extracts of Scurrula parasitica from different host trees in vitro.</p><p><b>METHOD</b>80% ethanol extracts of S. parasitica parasitizing on Nernium indicum, Morus alba, Opsmanthus fragrans, and Sapindus mulorossi were purified by polyamides column chromatography, and the eluates of 30%, 50%, 70% and 90% ethanol were mixed as flavonoids extracts. Human acute myeloid leukemia cell line HL-60 was used to evaluate the cytotoxicity induced by flavonoids extracts of S. parasitica L with MTT assay. Apoptosis was detected by AO/EB fluorescence staining and DNA fragmentation analysis, apoptosis rates and cell cycle distribution were detected by flow cytometry analysis.</p><p><b>RESULT</b>Extract of S. parasitica parasitizing on N. indicum (NISPEX) was the most sensitive to HL-60 cells of the 4 different host trees, the IC50 value being 0.60 mg x L(-1); and extract of S. parasitica parasitizing on M. alba took the second place, the IC50 value, being 2.49 mg x L(-1); extract of S. parasitica parasitizing on O. fragrans had no effectiveness as high as 50 mg x L(-1) concentration. NISPEX induced HL-60 cell apoptosis and inhibited the cell proliferation in dose and time-dependent manner. Cell cycles were arrested at G0-G1 phase after treated with NISPEX.</p><p><b>CONCLUSION</b>Anticancer effects of S. parasitica correlated with the host trees. Flavonoids extracts of S. parasitica parasitizing on N. indicum exhibited comparatively better anticancer activity in vitro among the host trees studied. NISPEX is found to be a good candidate for anticancer.</p>


Assuntos
Humanos , Apoptose , Ciclo Celular , Proliferação de Células , Fragmentação do DNA , Medicamentos de Ervas Chinesas , Química , Farmacologia , Flavonoides , Química , Farmacologia , Citometria de Fluxo , Células HL-60 , Leucemia , Tratamento Farmacológico , Loranthaceae , Química
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