Effects of oxypeucedanin on the resistance of breast cancer MCF-7/DOX cells to doxorubicin / 中国临床药理学与治疗学

AIM: To investigate the effect of oxypeucedanin (OPD) on doxorubicin resistance in human breast cancer MCF-7/DOX cells and its possible mechanism. METHODS: MCF-7/DOX cells were cultured in vitro, MTT assay was used to detect the effect of OPD on the survival of MCF-7/DOX cells, and the effect of OPD combined with different concentrations of doxorubicin on the proliferation of MCF-7/DOX cells were investigated. The effect of OPD combined with doxorubicin on the expression of genes including MDR1, MRP1, AGPAT2, CHKA, CEPT1, DGKA, PCYT1A, PLA2G15 in MCF-7/DOX cells was measured by qRT-PCR. The effect of OPD combined with doxorubicin on the protein expression of MDR1, MRP1, CHKA and CCTα in MCF-7/DOX cells was determined by Western blot. RESULTS: The IC

Comparative study on endoscopic bipolar coagulation hemostasis and nasal packing in the treatment of epistaxis / 中国基层医药

Objective To compare the therapeutic effect and clinical tolerance of endoscopic bipolar coagulation hemostasis(EBCH) and nasal packing(NP) in treatment of epistaxis.Methods The clinical data of 76 epistaxis patients treated with EBCH and 50 epistaxis patients treated with NP between Januaray 2009 and December 2011 were retrospectively analyzed.The two groups were matched in age,gender and bleeding sites.One time control rate of epistaxis,body tolerance,hospitalization time and recurrence rate were compared between the two groups.Results The one time control rate,body tolerance,hospitalization time and recurrence rate were 98.7％ (75/76),(2.91 ± 0.07),(4.11 ± 0.07) d and 2.63 ％ (2/76) respectively in EBCH group.The one time control rate,body tolerance,hospitalization time and recurrence rate were 72.0％ (36/50),(7.96 ± 0.10),(6.72 ± 0.13) d and 16.0％ (8/50) respectively in NP group.There were significant differences between the two groups.Conclusion The treatment of epistaxis with EBCH is better than NP on the therapeutic effect and clinical tolerance.The former method is a potential preferable choice for the treatment of clinical emergent epistaxis.

Efficacy and compliance of specific immunotherapy in allergic rhinitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the efficacy, safety and compliance of specific immunotherapy in allergic rhinitis.@*METHOD@#Two hundred and eighty cases with allergic rhinitis were undertaken immunotherapy, and then were observed its symptoms, signs and treatment by graded score.@*RESULT@#Total effective rate was 86.8%, 70.4%, 62.5% at 16 week, 6 months and 1 year after treatment. Of 280 patients treated, 74 patients stopped treatment after one year, compliance rate was 73.6%.@*CONCLUSION@#Specific immunotherapy is a safe and effective treatment methods for allergic rhinitis and worthy of attention and promotion, while strengthening the health education is needed to improve treatment compliance of patients.

The effect of the fluticasone propionate to the dynamic process of the nasal mucosal remodeling in allergic rhinitis of the rats model / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the dynamic process of the nasal mucosal remodeling, and the effect of the fluticasone propionate (FP) to remodeling, by establish animal model of allergic rhinitis (AR).@*METHOD@#One hundred and twenty Sprague-Dawley (SD) rats were randomly divided into three groups: the normal Group A used as controls and experimental groups: Group B and C, each group had 40 rats. After the animal model were established successfully by OVA+ Al (OH)3 and disposed, then, the dynamic process of the nasal mucosal remodeling was observed, through HE staining and transmission electron microscopic section in special times.@*RESULT@#The Group B, C nasal epithelium and cilia were not complete, eosinophil-based inflammatory cell infiltration, basement membrane thickening, collagen deposition, and a small amount of fibrosis could be found, but the structure of cells were not damaged. While those changes could not be observed in the Group A. The morphological changes of the nasal mucosa of Group B aggravated gradually under persistent allergen exposure, even stripped to the basement membrane in whole epithelial layers, cell and tissue structure were destroyed seriously. The morphological changes of nasal mucosa of Group C did not further increase, but still showed varying degrees of cilia arranged in uneven fashion, basement membrane thickening, collagen deposition and fibrous hyperplasia after treatment by FP.@*CONCLUSION@#Remodeling happens in the nasal mucosa, which would be aggravated, and even becomes irreversible if the allergen exposure continues persistently. The FP can relieve the clinical symptoms, slow down and even reverse the remodeling of AR. And it is ineffective when the changes become irreversible.

The dynamic expression of the TGF-beta1 and FGF-2 in the nasal mucosa in the allergic rhinitis rat models / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the dynamic expression of the TGF-beta1 and FGF-2 in the nasal mucosa of rat models of allergic rhinitis, at different stages of the process during pathogenesis.@*METHOD@#One hundred and eighty Sprague-Dawley (SD) rats were randomly divided into three groups, the normal Group A used for contrast and experimental groups: Group B and C, each group were 60 rats. Then, observed the expression of the TGF-beta1 and FGF-2 in the nasal mucosa at different stages of the process by immunohistochemical staining.@*RESULT@#The expression of the TGF-beta1 and FGF-2 in the nasal mucosa of the normal Group A was negative, while the Group B and Group C were varying degrees of positive both, and Group B, the level of the TGF-beta1 and FGF-2 were both decline after allergen avoidance, but still more not ability than the expression of the Group B (P < 0.05).@*CONCLUSION@#The expression of the TGF-beta1 and FGF-2 in the nasal mucosa of rats models were varying degrees of positive , and it is decline while the allergen avoidance.