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Objective:To study confirm whether curcumin could inhibit the proliferation of CD44+colon cancer cells. In this study. Methods: MTT method was used to test the proliferation inhibition effects of curcumin against colon cancer cells. And Real-time PCR and Fluorescence-activated cell sorting (FACS) were used to determine CD44 expression level in colon cancer cells after treated with curcumin. Then we used Magnetic-activated cell sorting ( MACS) method to separate CD44 positive and negative cells. And we compared the proliferation inhibition effects of curcumin against these two kinds of cells. Results: Curcumin could inhibit the proliferation of HCT116 and HT-29 colon cancer cell lines. The expression level of CD44 could also be down-regulated after the colon cancer cells were treated with curcumin. Conclusion: Curcumin could inhibit the proliferation of CD44+colon cancer cells. Thus it was suggested that crcumin might have the effect to inhibit the proliferation of colon cancer stem cells and might be used as a promising agents to prevent the relapse and metastasis of colon cancer.
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<p><b>OBJECTIVE</b>To investigate the effect and its mechanism of salmon milt DNA (SMD) on age-related involutions in mouse thymus.</p><p><b>METHODS</b>Female BALB/C mice aged 10 months were divided randomly into three groups according to their weights. They were high dosage group (333.33 mg.kg(-1).b.w.d(-1)), low dosage group (166.67 mg.kg(-1).b.w.d(-1)) and control group (0 mg.kg(-1).b.w.d(-1)). After five weeks, their thymus indexes were measured and the thymocytes were counted and the thymus cortex thicknesses were also measured using Image-Pro Plus (version. 4.0) software in the thymus section. All the data were analyzed by SAS statistic software. Microarray technique was applied to screen the gene fragments, which were differentially expressed between the high dosage group and the control group, together with RT-PCR to further confirm some of them.</p><p><b>RESULTS</b>No significant differences of the variables including body weight, thymus weight and thymus indexes among the three groups were found. The thymocytes quantities of thymus cortex and medulla in the high dosage group were significantly higher than those of the control group (P < 0.01 and P < 0.05, respectively). The thymus cortex thicknesses of both SMD supplement groups were significantly higher than that of the control group (P < 0.01 and P < 0.05 respectively). 112 differently expressed gene fragments were isolated. Furthermore, we found the fragments with the logged number of U23789, X80232 and Aw209102 were highly expressed in the high dosage group when RT-PCR technique was used.</p><p><b>CONCLUSIONS</b>SMD may reverse the age-related involutions in mouse thymus via up-regulation the expression of proliferation related genes and via up-regulation the expression of development and differentiation related genes simultaneously.</p>