Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Adicionar filtros








Intervalo de ano
1.
Chinese Journal of Surgery ; (12): 1070-1074, 2009.
Artigo em Chinês | WPRIM | ID: wpr-299764

RESUMO

<p><b>OBJECTIVE</b>To investigate the correlation between major histocompatibility complex (MHC) class I chain-related gene A (MICA) gene alleles matching rates and graft rejection in small intestine, liver and kidney transplantation.</p><p><b>METHODS</b>Genome DNA were extracted from blood samples or pathological sections collected from donors and recipients of living-related transplantation, included 4 cases of small bowel transplantation, 5 cases of liver transplantation and 6 cases of kidney transplantation. The correlation between MICA alleles matching rates and acute graft rejection was analyzed following 13 MICA alleles determination by polymerase chain reaction based on sequence-specific primers (PCR-SSP).</p><p><b>RESULTS</b>HLA zygosity of all donors and recipients was confirmed to be half-matching. The recipients displaying higher matching rates of MICA alleles with donors showed lighter clinical and pathological rejection and longer survival time. On the contrary, recipients with lower matching rates of MICA alleles with donors showed severer clinical and pathological rejection and shorter survival time relatively.</p><p><b>CONCLUSION</b>Matching rates of MICA alleles has negative relevance to acute rejection, and positive relevance to survival time of recipients in small bowel, liver, and kidney transplantation.</p>


Assuntos
Humanos , Alelos , Rejeição de Enxerto , Genética , Alergia e Imunologia , Antígenos de Histocompatibilidade Classe I , Genética , Intestino Delgado , Transplante , Transplante de Rim , Alergia e Imunologia , Transplante de Fígado , Alergia e Imunologia , Doadores Vivos , Transplante de Órgãos
2.
Chinese Journal of Surgery ; (12): 759-762, 2008.
Artigo em Chinês | WPRIM | ID: wpr-245534

RESUMO

<p><b>OBJECTIVE</b>To investigate the change in T cell-mediated immunity and its relationship with plasma high mobility group box-1 protein (HMGB1) levels in severely burned patients.</p><p><b>METHODS</b>Thirty-five extensively burned patients (> 30% total body surface area) were included in this study, and were divided into MODS group (n = 13) and non-MODS group (n = 22). The blood samples were collected on post burn days 1, 3, 5, 7, 14, 21 and 28. The plasma levels of HMGB1 were measured by using ELISA, and T lymphocyte proliferation response and its IL-2 production ability in peripheral blood were determined too. In addition, the ratio of CD4+/CD8+ T cells were detected by using flow cytometry.</p><p><b>RESULTS</b>Plasma HMGB1 levels were markedly elevated on post burn day 1 in severely burned patients, and HMGB1 level was significantly higher in MODS group than in non-MODS group (P < 0.05). Lymph proliferation response and IL-2 production of T cells in peripheral blood, and the ratio of CD4+/CD8+ T cells in MODS group were markedly lower than those in non-MODS group on post burn days 1, 14, 21 and 28 (all P < 0.05). It indicated that plasma HMGB1 levels were negatively correlated to T cellular immune function parameters, including lymphocyte proliferation response, IL-2 production, and the ratio of CD4+/ CD8+ T cells in extensively burned patients (all P < 0.05).</p><p><b>CONCLUSIONS</b>Extensive burns could lead to T cellular immune dysfunction, which appears to be associated with the development of MODS. HMGB1, as an important late mediators of inflammation, might be involved in the pathogenesis of suppression of T cell-mediated immunity in these patients.</p>


Assuntos
Adolescente , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Queimaduras , Sangue , Alergia e Imunologia , Proteína HMGB1 , Sangue , Imunidade Celular , Alergia e Imunologia , Insuficiência de Múltiplos Órgãos , Alergia e Imunologia , Linfócitos T , Alergia e Imunologia
3.
Acta Physiologica Sinica ; (6): 355-359, 2003.
Artigo em Chinês | WPRIM | ID: wpr-290960

RESUMO

In order to study the possible mechanism of CD226 monoclonal antibody (mAb)-mediated intracellular message transduction in human umbilical vein endothelial cells (HUVECs), the influence of CD226 mAb and its cross-linking by secondary antibody (II Ab) on the concentration changes in [Ca(2+)](i) in the HUVECs under different conditions were determined by confocal laser scanning microscopy. The main results are as follows. (1) When the culture medium was balanced by Hanks Buffer, [Ca(2+)](i) in HUVECs increased slowly after stimulation by CD226 mAb, whereas [Ca(2+)](i) increase was accompanied by [Ca(2+)](o) decrease after the mAb was cross-linked by goat anti-mouse IgG. Then [Ca(2+)](i) and [Ca(2+)](o) all returned to the normal level. (2) When the culture medium was balanced by D-Hanks buffer, [Ca(2+)](i) in HUVECs showed little variation when the cells were stimulated by CD226 mAb, but [Ca(2+)](i) decreased markedly after cross-linking. (3) When HUVECs were pretreated with EGTA, there was no variation in [Ca(2+)](i) of HUVECs after CD226 mAb stimulation alone or cross-linking of the mAb. Our results suggest that stimulation by CD226 mAb and cross-linking by goat anti-mouse IgG induce the variation of [Ca(2+)](i) in HUVECs under different conditions and the variation of [Ca(2+)](i) in HUVECs may play an important role in many physiological and pathological processes.


Assuntos
Humanos , Anticorpos Monoclonais , Farmacologia , Antígenos de Diferenciação de Linfócitos T , Alergia e Imunologia , Cálcio , Metabolismo , Células Cultivadas , Células Endoteliais da Veia Umbilical Humana , Metabolismo , Fisiologia
4.
Chinese Journal of Immunology ; (12)1986.
Artigo em Chinês | WPRIM | ID: wpr-674648

RESUMO

The immunoregulatory effect of TLSF_(JM) on the levels of IP3,Ca~(2+) in activated T cells andthe expression of Fos protein was investigated by ABC immunohistochemistry technique and Fu-ra-2/AM labelled T cells and anion-exchange chromatography techniques in this paper.The re-sults showed that TLSF_(JM) can the same time,it can strongly inhibit the levels of IP3、Ca~(2+) in ac-tivated T cells.

5.
Chinese Journal of Immunology ; (12)1985.
Artigo em Chinês | WPRIM | ID: wpr-674631

RESUMO

The regulatory effect of CD3 McAb on the human fetal thymocyte proliferative response toTPA was investigated.It was shown that the CD3 McAb couldn't induce human fetal thymo-cyte proliferative response,but suppressed PHA-induced human fetal thymocyte proliferativeresponse and promoted TPA-induced proliferative response,in a dose dependent manner.Ex-ogenous rHuIL—2,but not rHuIL—1 ,could enhance the proliferative response to CD3 McAb.These results have significances for understanding the expression of CD3 antigen and it's func-tions.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA