RESUMO
Oligozoospermia and asthenospermia are common causes of clinical male infertility. The number of patients increases year by year and shows a younger trend. Its etiology is complex， the mechanism and unclear pathogenesis and rely on empirical therapy. Therefore， it is necessary for exploring the pathogenesis and developing corresponding drugs to establish reasonable animal models. By comparing different animal model making methods， this paper provides ideas for constructing a more standardized animal model of oligoasthenospermia. At the moment， a lot of molding methods for oligoasthenospermia are available. Combined with the animal experimental articles of oligoasthenospermia in recent years， this study described the modeling with adenine， ornidazole， tripterygium glycoside， hydrocortisone， cyclophosphamide， busulfan， paclitaxel， heat stress， ionizing radiation， high-fat diet， and gene knockout， respectively， and compared the modeling methods in terms of the time， indexes， animal line， and model evaluation. Thereby， the advantages and disadvantages of different models of oligoasthenospermia were summarized， and finds that the existing animal models of oligoasthenospermia still have many shortcomings that need to be further improved. The selection， standardization and innovation of animal models need to be solved urgently， and the coincidence between animal models and clinical patients' traditional Chinese medicine syndromes is not coincident. In view of the existing problems， we should further explore how to build a modeling method in line with clinical characteristics and syndrome types， select the compound model method of integrated traditional Chinese and Western medicine， copy the model closer to the law of disease development and in line with traditional Chinese medicine syndrome， and provide animal experimental support for exploring the mechanism of disease， developing characteristic drugs and guiding clinical medication.
RESUMO
OBJECTIVE:To examine the effects of sanazole and of sanazole combining with ?-ray irradiation on the colony formation ratio of granuloid/macrophage committed progenitor cell(CFU-GM)from mouse bone marrow in vivo.METHODS:(1)After a series of doses of sanazole solution(50,100,200mg/kg)was given to mice intravenously,CFU-GMs were separated from mouse bone marrow for evaluating their colony formation ratio.(2)To evaluate the radiosensitization of sanazole,a series of doses of sanazole solution(50,100,200mg/kg)was given to mice intravenously 30min before the mice were exposed to ?-ray irradiation in different doses(5,10,20Gy)respectively.After each treatment,CFU-GMs were separated from mice marrow for counting the ratio of colony formation.RESULTS:(1)Sanazole especially in large doses didn’t showed considerable cytotoxicity to CFU-GM colony formation in mice in vivo.(2)The inhibiting effect of a series of doses of sanazole solution combined with a series of doses of radiation (5,10,20Gy)on colony formation of CFU-GM from mice bone marrow was the same as that of radiation alone.CONCLUSION:Large doses of sanazole showed no obvious effect on the colony formation of CFU-GM of mouse bone marrow in vivo.No radiosensitization to the colony formation of CFU-GM was found in vivo when a series of doses of sanazole in combination with radiation were used.