RESUMO
ABSTRACT Four compounds including beauvericin, parahydroxybenzaldehyde, indole-3-carboxylic acid and quinizarin were isolated from endophytic fungus Epicoccum nigrum and their cytotoxicity, antibacterial and antioxidant activity were evaluated. Beauvericin had remarkable activity against two Gram-negative strains (Bacillus cereus and Salmonella typhimurium) with respective MIC values of 3.12 and 6.25 µg/ml. All the compounds had weak cytotoxic effect on both normal and tumor cells. LC50 values ranged from 40.42 to 86.56 µg/ml, 31.87 to 86.57 µg/ml and 21.59 to 67.27 µg/ml on Vero cells, THP-1 and RAW 264.7 respectively. The present study showed that these compounds could be developed for the formulation of antioxidant-rich therapeutic diets and as a therapeutic agent against bacterial infections.
RESUMO
Ethyl acetate (EA) extract of the stem bark of Cylicodiscus gabunensis (CG) was analysed phytochemically and evaluated for its antimicrobial activity against 17 pathogenic species isolated from patient: Escherichia coli; Klebsiella pneumoniae; Shigella dysenteriae; Shigella flexneri; Morganella morganii; Proteus vulgaris; Proteus mirabilis ; Salmonella typhi; Citrobacter freundii; Enterobacter cloacae; Enterobacter agglomerans; Staphylococcus aureus; Streptococcus feacalis; Pseudomonas aeruginosa; Bacillus cereus T; Candida albicans and Candida glabrata. Flavonoids; saponins; tannins; polyphenols; coumarins; triterpenes and/or sterols and reducing sugars were detected in the (EA) extract of CG. The best MIC and MBC values for the microorganisms sensitive to the extract were 0.00078 and 0.00315 mg/ml respectively. The greater and remarkable antimicrobial activity of the (EA) extract of CG was recorded with Staphylococcus aureus; Proteus vulgaris and Bacillus cereus T. These results provide a rationalization for the traditional use of this plant for the treatment of infections diseases