RESUMO
Cancer is an important disease that causing to deaths in the world. Cervical cancer is one of the most common among women and must be treated quickly to prevent cell proliferation. Natural products have been used for cancer treatment due to their antiproliferative and apoptosis induction properties. In the present study, we aimed to determine the antiproliferative and apoptosis induction activities of methanol extract of Inula graveolens (IGME) in the human cerival cancer cell line (HeLa). Antiproliferative activity of IGME was evaluated by using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) method. Apoptosis induction activity was determined by AnnexinV/propodium iodide staining with FACS. ROS induction was determined by DCFH-DA (2',7'-Dichlorofluorescin diacetate) staining. Interleukin 6 and 8 levels (IL-6 and IL-8) were determined by the Elisa method. IGME exhibited antiproliferative effect and induced apoptosis in the HeLa cells. IL-6, IL-8 and intracellular ROS levels were decreased after treatment of IGME. Consequently, IGME was found to have antiproliferative and apoptosis induction activities as an indicator of the anticancer activity.
El cáncer es una enfermedad importante que causa muertes en el mundo. El cáncer de cuello uterino es uno de los más comunes entre las mujeres y debe tratarse rápidamente para evitarla proliferación celular. Los productos naturales se han utilizado para el tratamiento del cáncer debido a sus propiedades anti proliferativas y de inducción de la apoptosis. En el presente estudio, nuestro objetivo fue determinar las actividades de inducción de apoptosis y antiproliferativas del extracto de metanol de Inula graveolens (IGME) en la línea celular de cáncer de cuello uterino humano (HeLa). La actividad antiproliferativa de IGME se evaluó utilizando el método MTT (bromuro de 3-[4,5-dimetiltiazol-2-il]-2,5-difenil-tetrazolio). La actividad de inducción de apoptosis se determinó mediante tinción con anexina V/yoduro de propodio con FACS. La inducción de ROS se determinó mediante tinción con DCFH-DA (diacetato de 2',7'-diclorofluoresceina). Los niveles de interleucina 6 y 8 (IL-6 e IL-8) se determinaron por el método Elisa. IGME exhibió un efecto antiproliferativo e indujo apoptosis en las células HeLa. Los niveles de IL-6, IL-8 y ROS intracelulares disminuyeron después del tratamiento con IGME. En consecuencia, se encontró que IGME tenía actividades antiproliferativas y de inducción de apoptosis como indicador de la actividad anticancerígena.
Assuntos
Plantas Medicinais , Neoplasias do Colo do Útero/tratamento farmacológico , Inula , Apoptose , Antineoplásicos/farmacologiaRESUMO
The breast cancer is one of the most common types of cancer. Many scientists have focused on the treatment of this disease for a long time by studying anti-cancer activities of plant extracts as well as synthetics. Lamiaceae and Asteraceae have been used in anticancer studies due to their phytochemical content. The genus Sideritis, Achillea and Tanacetumare the members of these families. Sideritis, Achillea and Tanacetum are used as herbal medication for the treatment ofvariety of diseases. In present study, we demonstrated the biological activity of Sideritis syriaca (SS), Achillea aleppica (AAZ) and Tanacetum argenteum (TAA) methanol extracts on cell viability of the breast cancer line MCF7. MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to determine the cell viability and proliferation of MCF7 cells. In a dose dependent manner, methanol extracts (0, 1, 5, 25, 100 and 250 μg/ml) of SS, AZZ and TAA were examined on MCF7, and viability of cells were determined with MTT staining. Especially, concentrations in 100 and 250 μg/ml of extracts decreased the cell viability (p<0.001). The results of the current study showed that methanol extracts of SS, AZZ and TAA effectively inhibited the cell proliferation by decreasing the cell viability of MCF7 cells. Suggesting that SS, AZZ and TAA can be considered as natural herbal-based anti-cancerous agents.