The crambe (Crambe abyssinica Hochst) byproducts, can be used as a source of non-degradable protein in the rumen? / Coprodutos de crambe (Crambe abyssinica Hochst) podem ser utilizados como fonte de proteina não degrádavel no rúmen?

To evaluated the chemical composition and ruminal degradability of crambe byproducts (meal and crushed) and proteic supplements formulated with crushed crambe (0; 2.5; 5; 10 and 15%); five crossbred steers with average weight of 485±14 kg, were used. All the animal were kept in individual paddocks of 0.25 ha on Urochloa brizantha (syn. Brachiaria brizantha). It was observed a greater soluble fraction, higher effective degradability at 5%/h and higher degradation rate "c" and, consequent, lower indigestible fraction for crambe crushed ground in the sieve of 3 mm. The effective degradability at 5%/h was lower for the crambe crushed (55.42) in relation to the meal (48.80). The diet with 5% of inclusion of crambe showed higher effective degradability for dry matter (54.86%) and lower fraction "I" (30.64%) associated with higher fractions "c" and "b". Crambe ground in sieves of 1 and 3 mm mesh presented the highest degradability. Crushed crambe showed higher ruminal degradation than crambe meal; the crambe byproducts possibility can be use as a source of non-degradable protein in the rumen.

Para se avaliar a composição química, degradabilidade ruminal, o tempo da colonização da torta e do farelo de crambe e de suplementos protéico compostos de torta de crambe (0; 2,5; 5,0; 10 e 15%); foram utilizados cinco novilhos mestiços, com peso médio de 485 ± 14 kg. Todos os animais foram mantidos em piquetes individuais de 0,25 ha em pastagens de Urochloa brizantha (syn. Brachiaria brizantha). Observou-se uma fração solúvel maior, maior degradabilidade efetiva para a taxa de passagem de 5% / h e maior taxa de degradação "c" e, consequentemente, menor fração indigerível para a torta de crambe moído na peneira de 3 mm. A degradabilidade efetiva a 5% / h foi menor para a torta de crambe (55,42) em relação ao farelo (48,80). A dieta com adição de 5% de torta de crambe apresentou maior degradabilidade efetiva da matéria seca (54,86%) e menor fração "I" (30,64%), associada com as frações mais elevadas "c" e "b". A torta de Crambe moída em peneiras de 1 e 3 mm de diâmetro apresentaram os maiores valores de degradabilidade. A torta de apresenta maior degradação ruminal que o farelo de crambe. Os coprodutos de crambe possivelmente podem ser usados como fonte de proteína não degradada no rúmen.

Influence of starch and casein administered postruminally on small intestinal sodium-glucose cotransport activity and expression

Twenty-four Holstein steer calves (88 ± 3kg) with abomasal catheters were randomly assigned within blocks to one of four treatments. The treatments consisted of four abomasal infusions: water (control), 4 g/kg BW.d-1 of partially hydrolyzed starch (SH), 0.6 g/kg BW.d-1 of casein, and a mix of SH + casein. The small intestine was collected and five equidistan, 1m sites were identified (duodenum, jejunum 1, jejunum 2 jejunum 3 and ileum). Maltase specific activity in mucosal homogenate and brush border membrane vesicles, SGLT1 protein abundance, and sodium-dependent glucose uptake in brush border membrane vesicles did not differ between the calves receiving different abomasal infusion treatments. However, maltase specific activity in homogenates and brush border membrane vesicles increased four-fold from the duodenum to the first jejunal site before declining steadily towards the ileum (p=0.0145 p=0.0020, respectively). The SGLT1 abundance differed by intestinal sampling site (p=0.0162). These data indicated that cattle might not have the ability to alter the capacity for glucose uptake when challenged with different substrates and that the regulation of SGLT1 expression could differ between bovine and other species.