RESUMO
Objective: To investigate whether there is an association between the 45bp insertion/deletion polymorphism in the uncoupling protein 2 [UCP2] gene, and obesity and/or obesity-related phenotypes
Design: A prospective analytical case control survey
Setting: Afyon Kocatepe University Medical Faculty and Afyonkarahisar State Hospital, Turkey
Subjects: A total of 138 obese cases and 75 non-obese controls from Western population of Turkey were included in this study
Interventions: Anthropometric data were obtained by measurements of weight, height and circumferences of waist; and hip and body mass index and waisthip ratio [WHR] were calculated. DNA was isolated from peripheral blood and statistical analysis was conducted using the SPSS programme [version 17.0]. The genotypes for the UCP 45 bp ins/del polymorphism were identified by polymerase chain reaction
Main outcome measures: The relationship between the 45bp insertion/deletion polymorphism in the UCP2 gene and obesity and/or obesity related phenotypes
Results: No significant difference was detected between obese cases and non-obese controls regarding genotype distribution and allele frequencies. However, the WHRs from phenotypes among obese cases having Del/Del and Ins/Del genotypes were found to be respectively higher [0.87 +/- 0.09 and 0.90 +/- 0.10] than in the ones having Ins/Ins genotypes [0.81 +/- 0.13] [p <0.05]
Conclusions: This is the first study supporting the role of the 45 bp insertion/deletion polymorphism in obesity in Turkey. Further studies with larger sample sizes may confirm these findings