Dose estimation with the calibration of dose-response curve of micronucleus in human peripheral lymphocytes induced by 50MeV proton beams

The purpose of this paper is to establish an easy and reliable biodosimeter protocol to evaluate the biological effects of proton beams. Human peripheral blood lymphocytes were irradiated using proton beams [LET: 34.6 keV micro m[-1]], and the chromosome aberrations induced were analyzed using cytokinesis-blocked [CB] micronucleus [MN] assay. To determine the efficiency of MN assay in estimating the doses received by 50MeV proton beams and to monitor predicted dose of victims in accidental exposure, here we have evaluated the performance of MN analysis in a simulated situation after exposure with proton beams. Peripheral lymphocytes were irradiated by 50MeV proton beams up to 6Gy and analyzed by Giemsa staining of CB MN assay. The detected MN was found to be a significant dose-effect curve in the manner of dose-dependent increase after exposure with proton beams in vitro. When plotting on a linear scale against radiation dose, the line of best fit was Y=0.004+[1.882x10[-2] +/- 9.701x10[-5]] D+[1.43x10[-3] +/- 1.571x10[-5]]D2. Our results show a trend towards increase of the number of MN with increasing dose. It was linear-quadratic and has a significant relationship between the frequencies of MN and dose [R2= 0.9996]. The number of MN in lymphocyte that was observed in control group is 5.202 +/- 0.04/cell. Hence, this simple protocol will be particularly useful for helping physicians to decide medical therapy for the initial treatment of victims with rapid and precise dose estimation after accidental radiation exposure. Also it has potential for use as a valuable biomarker to evaluate the biological effectiveness for cancer therapy with proton beams

Annual Report on External Quality Assessment in Inborn Error of Metabolism in Korea (2003) / 임상검사와정도관리

The trial of external quality assessment for inborn error of metabolism was performed in 2003. A total 10 specimens for neonatal screening tests were distributed to 43 laboratories with a response rate of 83%. All the control materials were sent as a filter paper form. Each laboratory replied the test result as the screening items they were doing as a rountine test at the reception of the specimen among PKU screening, neonatal TSH, neonatal T4(total/free), galactosemia screen, homocytinuria screen and histidinemia screen. The mean, SD, and CV were analyzed.

Effect of Acupuncture on Histamine Induced Itch / 대한피부과학회지

We cairried out this study to find the possibility of itch control with acupuncture under the hypothesis that antipruritic effect also exists if the itch sensation is closely allied to pain. We evaluated the effect of acupuncture on histarnine induced itch, flare and wheal in 20 healthy male volunteers aged 24 32 years old and compared it with the effect of pseudoacupuncture on them and that of no intervention on them. The results were as follows: 1. Mans of itch intensity and duration were decreased in A(session with acupuncture) and P(session with Pseudoacupuncture), compared to those of N(session with no intervention), and A was more effective in decreasing the itch intensity and its duration than P. 2. Means of maximal sizes of flare and wheal were smaller in A and P, but there was no remarkable difference between A and P.