Preparation and quality evaluation of hydroxysafflor yellow A solid lipid nanoparticles / 国际中医中药杂志

Objective To optimize the formulation and preparation technology of hydroxy safflower yellow A solid lipid nanoparticles and evaluate its quality. Metheds Hydroxy safflower yellow A solid lipid nanoparticles were prepared by the method of hot melt emulsification ultrasonic-low temperature using hydroxypropyl methylcellulose as a lipid material and glyceryl monostearate as an emulsifier. Using entrapment efficiency as indexes, the amount of hydroxypropyl methylcellulose, purified water, glyceryl monostearate, and Tween 80 aqueous solution (1%) as factors, orthogonal test was applied to optimize the formulation and preparation technology. Dialysis method was used to measure encapsulation efficiency. The morphology and uniformity of the nanoparticles were observed by transmission scanning electron microscopy. The particle size, polydispersion index and Zeta potential were determined by nano-particle size analyzer. And hydroxy safflower yellow A solid lipid nanoparticles sustained releasing characteristics was evaluated by the percentage of cumulative drug release. Results The optimal process of prepared hydroxy safflower yellow A solid lipid nanoparticles was 5 mg of hydroxypropyl methylcellulose, 0.2 ml of purified water, 100 mg of glyceryl monostearate, and 1.8 ml of Tween 80 aqueous solution (1%). The size of the prepared nanoparticles was uniform and spherical. And the average particle size were (99.85 ± 3.04) nm, polydispersion index were (0.390 ± 0.021), Zeta potential were (-27.63 ± 2.12) mV, and the encapsulation efficiency of the hydroxy safflower yellow A solid lipid nanoparticles were (63.35 ± 2.65)%. The release rate of the nanoparticles was (44.35 ± 0.49)%. Conclusions The prepared hydroxy safflower yellow A solid lipid nanoparticles have good uniformity and good sustained release properties.

Preparation and quality evaluation of hydroxysafflor yellow A solid lipid nanoparticles / 国际中医中药杂志

Objective@#To optimize the formulation and preparation technology of hydroxy safflower yellow A solid lipid nanoparticles and evaluate its quality.@*Metheds@#Hydroxy safflower yellow A solid lipid nanoparticles were prepared by the method of hot melt emulsification ultrasonic-low temperature using hydroxypropyl methylcellulose as a lipid material and glyceryl monostearate as an emulsifier. Using entrapment efficiency as indexes, the amount of hydroxypropyl methylcellulose, purified water, glyceryl monostearate, and Tween 80 aqueous solution (1%) as factors, orthogonal test was applied to optimize the formulation and preparation technology. Dialysis method was used to measure encapsulation efficiency. The morphology and uniformity of the nanoparticles were observed by transmission scanning electron microscopy. The particle size, polydispersion index and Zeta potential were determined by nano-particle size analyzer. And hydroxy safflower yellow A solid lipid nanoparticles sustained releasing characteristics was evaluated by the percentage of cumulative drug release.@*Results@#The optimal process of prepared hydroxy safflower yellow A solid lipid nanoparticles was 5 mg of hydroxypropyl methylcellulose, 0.2 ml of purified water, 100 mg of glyceryl monostearate, and 1.8 ml of Tween 80 aqueous solution (1%). The size of the prepared nanoparticles was uniform and spherical. And the average particle size were (99.85 ± 3.04) nm, polydispersion index were (0.390 ± 0.021), Zeta potential were (-27.63 ± 2.12) mV, and the encapsulation efficiency of the hydroxy safflower yellow A solid lipid nanoparticles were (63.35 ± 2.65)%. The release rate of the nanoparticles was (44.35 ± 0.49)%.@*Conclusions@#The prepared hydroxy safflower yellow A solid lipid nanoparticles have good uniformity and good sustained release properties.

Effects of timosaponin B-II on differentiation of rat neural stem cells into tyrosine hydroxylase posi-tive neurons in vitro / 中华行为医学与脑科学杂志

Objective To explore the effect of timosaponin B-II ( TB-II) on the differentiation of neural stem cells (NSCs) into tyrosine hydroxylase (TH) positive neurons in neonatal rats. Methods The biological functions of self-proliferation and multi-differentiation of NSCs were identified by primary culture, cell proliferation counting,morphological observation and immunology. NSCs of SD rats were cultured in vitro and treated with different concentrations of TB-II (10 μg/ml,30 μg/ml ,100 μg/ml) for 7 days. Immuno-histochemistry was used to detect the effect of TB-II on the differentiation of NSCs into TH-positive neurons, and Western blot was used to detect the expression of TH protein in neurons. Results ( 1) The cultured cells had the ability to self-proliferation,expressed nestin protein and differentiated into neurons and glial cells. So the cultured cells were conformed to the biological function of neural stem cells. (2)Compared with the control group,the TH positive cell ratio of TB-II 30 μg/ml group and TB-II 100 μg/ml group increased ((10. 03± 1. 36)%),( 20. 01± 3. 37)%),(31. 32± 3. 98)%) ,the difference was significant ( t=6. 15, 16. 54,both P<0. 05). There was no significant difference between TB-II 10 μg/ml group and control group (P>0. 05). (3)Western results showed that the relative expression of TH protein in TB-II 30 g/ml group and TB-II 100 μg/ml group was higher than that in control group,the difference was statistically significant (con-trol group: (1. 02±0. 24),TB-II 30μg/ml group: (3. 64±1. 78),TB-II 100 μg/ml group: (5. 88±2. 34);t=12. 58,9. 15,both P<0. 05). There was no significant difference between TB-II 10 μg/ml group and con-trol group (P>0. 05). Conclusion TB-II can promote the differentiation of NSCs into TH-positive neurons.

ULTRASTRUCTURE STUDIES ON ERYTHROID LINE IN THALSSEMAIA / 重庆医科大学学报

Ultrastructure varities in erythroid line of 6 patients with Thalassemia were studied by electron microscopy. A number of electron-dense inclusions (alpha or beta chains precipitation) were noted in cytoplasm or nuclei of polychromatic erythroblasts, normoblasts, reticulocytes and erythrocytes. The nuclear membranes showed absence or duplication and the space between two layers of nuclear membranes dilated. Autophage vacuoles and vacuolation were easily observed within the early and late stages of erythroid series. The figure of red cells was markedly abnormal, the characteristic ropalocytes might be found. In addition, the various forms of squeezed and partly destructed RBC which had been sequestrated in the minute sinuses of spleen were observed. The observations above provide direct morpdological evidences of the pathogenesis in Thalassemia

A MORPHOLOGIC STUDY OF PULMONARY HYPOPLASIA IN FETAL RABBITS WITH EXPERIMENTAL DIAPHRAGMATIC HERNIA / 重庆医科大学学报

Experimental diahragmatio hernia was created on fetal rabbits during gestaional day (GD) 23-25d, and 8 rases of the model survived in intrauterin'j life up to the full term -were obtained by hysterotomy for morphologic study on pulmonary hypoplsia. At the samatime 20 normal f

YOLK SAC TUMUR IN CHILDREN / 重庆医科大学学报

37 cases of yolk sac tumor were treated in our hospital from 1966-1989. Serum AFP were measured. The diagnosis of yolk sac tumor was confirmed by light microscope, 10 cases were further examined with electron microscope and 8 cases with AFP immunohistochemistry study. Related literature reviewed. These studies confirm the concept suggested by Telium. i. e. histogenesis of the tumor arising from germ cell differentiating along dorsal wall of yolk sac.1. AFP had been synthesized in yolk sac of embryo. On our study AFP elevated predominately and had been localized by immunoenzymatic labelling PAP methods.2. Electron microscope examination showed that morphology of this tumor is similar with normal human yolk sac at 7 weeks gestation and those finding demonstrated epithilial and mesothelial element is the same as the extia-embryonic endoderm and mesoderm.The terminology of this tumor, the associated relation with teratoma and practice of immunohistochemistry have been discussed