RESUMO
Objective To detect different animal erythrocyte hemolysins titer,and compare the application of these hemolysins in immunological experimental teaching,for selecting the better method of preparing high titer hemolysin for experimental teaching of medical immunology.Methods A total of 40 experiment rabbits were divided into 4 groups in this study,and immunized by sheep red blood cell(SRBC) and porcine red blood cell(PRBC) through different immunization procedures to prepare the hemolysin,detect and compare these 4 groups hemolysins titer by the complement hemolysis test.Results Rabbit Anti-SRBC in the group A was 1∶4 800,rabbit Anti-PRBC in the group B was 1∶1 200,rabbit Anti-SRBC in the group C was 1∶1 000,rabbit Anti-PRBC in the group D was 1∶200.Conclusion The hemolysin titer of the rabbit Anti-PRBC was lower than that of the rabbit Anti-SRBC by the same immunization procedures,and the immunization procedure by intradermal multi-point and auricular vein injection is the better method of preparing high titer hemolysin,so PRBC could replace SRBC as antigen,and immunize the rabbits for preparing hemolysin,which could be used in experimental teaching of medical immunology.
RESUMO
Objective To evaluate the effect of flurbiprofen axetil pretreatment on mesenteric trac-tion syndrome in elderly patients undergoing abdominal surgery. Methods Sixty patients of both sexes, aged 65-86 yr, with body mass index of 18.8-25.6 kg∕m2, of American Society of Anesthesiologists phys-ical status Ⅰor Ⅱ, scheduled for elective gastrointestinal tract surgery under general anesthesia, were di-vided into 2 groups(n=30 each)using a random number table: flurbiprofen axetil group(group F)and normal saline group(group NS). Flurbiprofen axetil 50 mg was intravenously injected during anesthesia in-duction in group F, while the equal volume of normal saline was given instead in group NS. Immediately af-ter anesthesia induction, immediately after mesenteric traction and at 10, 15 and 30 min after mesenteric traction, arterial blood samples were collected for measurement of plasma 6-keto-prostaglandin F1α concen-trations using enzyme-linked immunosorbent assay. The occurrence of mesenteric traction syndrome was re-corded within 30 min after mesenteric traction. Results Compared with group NS, the plasma 6-keto-pros-taglandin F1α concentrations were significantly decreased at each time point, and the incidence of mesen-teric traction syndrome was decreased during each period after mesenteric traction in group F(P<0.05). Conclusion Flurbiprofen axetil pretreatment can effectively prevent the development of mesenteric traction syndrome in elderly patients undergoing abdominal surgery.