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Guided by the development of new medical science proposed by Ministry of Education and the "Education and Training Plan for Excellent Doctors 2.0", shifted from treatment-oriented to whole life-health cycle, we have explored curriculum ideological and political education in metabolic-related curriculum chain. Firstly, we constructed a core teaching team and had the training of curriculum ideological and political education. The top-level design was made with the integration of moral education into medical education. Secondly, the syllabus was comprehensively revised, containing the connotation of "morality education". The elements relevant to curriculum ideological and political education hidden behind professional courses were excavated. Finally, the mixed teaching mode of online combining with offline was carried out. Metabolism-related curriculum chain, focused on "metabolism, diabetes, obesity and patient education", formed a progressive link from basic medical science to practice to clinical, strengthening the "prevention, treatment and health care" based "one health" philosophy and giving full play to the implicit curriculum ideological and political education hidden behind professional courses. Our practice shows that the implementation of curriculum ideological and political education in metabolism-related curriculum has been accepted by students, and curriculum ideological and political education has been become part of professional courses. The "gene chimera" mode for curriculum ideological and political education incorporation into professional courses needs to be infiltrated imperceptibly, and the effect will be visualized in the future.
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Objective To investigate the relationship between the initial change of thyroglobulin (Tg) and clinical outcome in differentiated thyroid carcinoma (DTC) patients with pulmonary metastases after 131I treatment. Methods A total of 69 DTC patients with pulmonary metastases from January 2010 to December 2015 were retrospectively analyzed. Patients were divided into 3 groups according to the variation of Tg: groupⅠ(Tg declined≥ 50%), groupⅡ(Tg declined＜50% or Tg increased＜10%), and group Ⅲ (Tg increased ≥10% ). The follow-up time was (49.2 ± 9.3) months. Clinical outcomes were divided into remission, stable disease and progressive disease according to the serum test and imaging results. Results The percentage of group Ⅰ, Ⅱ, Ⅲ patients was 44.9% (31/69), 40.6% (28/69), and 14.5% (10/69) respectively. Results of follow-up showed 19.4% (6/31) patients achieved remission and 80.6% (25/31) had stable disease in groupⅠ. There were 10.7% (3/28) patients with remission, 60.7% (17/28) with stable disease and 28.6% (8/28) with progression disease in groupⅡ. All patients showed progressive disease in groupⅢ. The clinical outcome was related to the variation of Tg after 131I treatment (P＜0.01). Conclusions Initial Tg after 131I treatment could be a predictor to the outcome of patients. The increased Tg level indicates a high possibility of 131I refractory disease.
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Objective To investigate the impact of nursing intervention on the prevention of deep venous thrombosis after orthopaedic surgery of lower extremities.Methods 104 patients were selected from our hospital after orthopaedic surgery of lower extremities were randomly divided into the control group and the observation group with 52 cases in each group,the control group was given normal routine care,the observation group received target-oriented nursing.The incidence of deep venous thrombosis was compared between the two groups.Results The rate of deep venous thrombosis in the observation group was significantly lower than that in the control group.Conclusions Through targeted nursing intervention,we can effectively prevent deep venous thrombosis after orthopaedic surgery of lower extremities,so it deserved promotion and application in clinic.
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@#目的探讨康复训练配合针刺治疗脑卒中患者吞咽功能障碍的疗效。方法 87例脑卒中伴吞咽功能障碍者分为综合治疗组(56例)和对照组(31例)。综合治疗组进行针灸和康复训练 ,对照组仅进行内科常规治疗。结果综合治疗组与对照组在统计学上有非常显著性差异(P<0.01)。结论脑卒中吞咽功能障碍患者早期进行综合康复能促进功能恢复、减少鼻饲并发症。
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Objective:To construct a recombinant retroviral vector expressing pancreatic duodenal homeobox-1(PDX-1) gene and express the PDX-1 expression cassette in liver progenitor cells(LEPCs),so as to study the conversion of hepatic cells to pancreatic-like cells.Methods: The full length of PDX-1 gene was amplified by PCR and subcloned into pMSCVpuro vector.The pMSCV PDX-1 puro was then introduced into Phoenix package cells and the cell culture supernatant was used for Ping-Pong infection of another packaging cell line PT67 to obtain stable virus-producing cell line.Results: The pMSCV PDX-1 puro vector was successfully constructed and transfected into PT67 cells.The viral supernatants of PT67 cells efficiently infected LEPCs in vitro and the infected LEPCs stably expressed PDX-1 gene.Conclusion: The pMSCV PDX-1 puro vector and PDX-1 expressing LEPCs have been successfully constructed,laying a basis for studying the transdifferentiation of liver progenitor cells to pancreas lineage cells.
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Objective:To construct a recombinant retroviral vector harboring MafA and to establish a liver epithelial progenitor cell(LEPC)line for stable expression of MafA.Methods:MafA was amplified by PCR and suncloned into pBMN-Z- IRES-Neo vector to obtain pBMH-MafA-Neo vector.After introducing the pBMN-MafA-Neo into Phoenix package cells,the cell culture supernatant was used to infect LEPCs.LEPCs stably expressing MafA gene were screened out.RT-PCR was used to detect the influence of MafA on the phenotype of LEPCs.Results:We successfully constructed pBMH-MafA-Neo vector and obtained LEPCs which stably expressed MafA.Expression of GK and GLUT2 genes in LEPCs-MafA cells was higher than that in the LEPCs.Conclusion:We have successfully obtained LEPCs which can stably express MafA,laying a basis for studying the differentiation of LEPCs into pancreas cells.