Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Adicionar filtros








Intervalo de ano
1.
Indian J Med Microbiol ; 2015 Jan-Mar ; 33 (1): 101-109
Artigo em Inglês | IMSEAR | ID: sea-156998

RESUMO

Purpose: Different approaches have been used for preventing biofi lm-related infections in health care settings. Many of these methods have their own de-merits, which include chemical-based complications; emergent antibiotic resistant strains, etc. The formation of biofi lm is the hallmark characteristic of Staphylococcus aureus and S. epidermidis infection, which consists of multiple layers of bacteria encased within an exopolysachharide glycocalyx. Nanotechnology may provide the answer to penetrate such biofi lms and reduce biofi lm formation. Therefore, the aim of present study was to demonstrate the biofi lm formation by methicillin resistance S. aureus (MRSA) and methicillin resistance S. epidermidis (MRSE) isolated from wounds by direct visualisation applying tissue culture plate, tube and Congo Red Agar methods. Materials and Methods: The anti-biofi lm activity of AgNPs was investigated by Congo Red, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) techniques. Results: The minimum inhibitory concentration (MIC) was found to be in the range of 11.25-45 μg/ml. The AgNPs coated surfaces effectively restricted biofi lm formation of the tested bacteria. Double fl uorescent staining (propidium iodide staining to detect bacterial cells and fl uorescein isothiocyanate concanavalin A (Con A-FITC) staining to detect the exopolysachharides matrix) technique using CLSM provides the visual evidence that AgNPs arrested the bacterial growth and prevent the glycocalyx formation. In our study, we could demonstrate the complete anti-biofi lm activity AgNPs at a concentration as low as 50 μg/ml. Conclusions: Our fi ndings suggested that AgNPs can be exploited towards the development of potential anti-bacterial coatings for various biomedical and environmental applications. In the near future, the AgNPs may play major role in the coating of medical devices and treatment of infections caused due to highly antibiotic resistant biofilm.

2.
Braz. j. microbiol ; 40(4): 916-922, Oct.-Dec. 2009. tab
Artigo em Inglês | LILACS | ID: lil-528175

RESUMO

A few mixed ligand transition metal carbodithioate complexes of the general formula [M(4-MPipzcdt)x(phen)y]Y (M = Mn(II), Co(II), Zn(II); 4-MPipzcdt = 4-methylpiperazine-1-carbodithioate; phen = 1,10-phenanthroline; x = 1 and y = 2 when Y = Cl; x = 2 and y = 1 when Y = nil) were synthesized and screened for their antimicrobial activity against Candida albicans, Escherichia coli, Pseudomonas aeruginosa,Staphylococcus aureus andEnterococcusfaecalis by disk diffusion method. All the complexes exhibited prominent antimicrobial activity against tested pathogenic strains with the MIC values in the range <8-512 ìgmL-1. The complexes [Mn(4-MPipzcdt)2(phen)] and [Co(4-MPipzcdt)(phen)2]Cl inhibited the growth of Candida albicans at a concentration as low as 8 µgmL-1.The complexes were also evaluated for their toxicity towards human transformed rhabdomyosarcoma cells (RD cells). Moderate cell viability of the RD cells was exhibited against the metal complexes.


Assuntos
Fenantrolinas/análise , Metais/análise , Toxicidade/análise , Ligantes
3.
J Biosci ; 1990 Dec; 15(4): 313-322
Artigo em Inglês | IMSEAR | ID: sea-160854

RESUMO

Using a bacterial species Pseudomonas PG-1, evidence has been obtained which indicates that uptake of n-pentane to n-octane by microbial cells takes place primarily from the gas phase either directly or via the aqueous phase. Specific growth rate increased along with the increase in substrate concentration but above the alkane concentration of 0·3% by volume, specific growth rate decreased indicating substrate inhibition of growth. In the case of less volatile alkanes, n-nonane and n-decane, substrate transfer is predominantly through substrate solubilization system elaborated by the cells. EDTA, a strong inhibitor of hydrocarbon solubilization by the cells, inhibited growth on these two alkanes but had negligible effect on growth on n-pentane to n-octane.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA