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1.
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 597-601, 2014.
Artigo em Chinês | WPRIM | ID: wpr-312770

RESUMO

<p><b>OBJECTIVE</b>To investigate the effect of Jiangtang Yishen Recipe (JTYSR) on high insulin induced cell proliferation of human glomerular mesangial cells (HMCs) and the expression of insulin receptor substrate 1 (IRS-1) and phosphatidylinositol-3-kinase (PI-3K).</p><p><b>METHODS</b>HMCs were divided into 4 groups, i.e., the negative control group, the high insulin model group, the JTYSR group, and the LY294002 group. The concentration of insulin, JTYSR, and LY294002 was respectively confirmed by pre-experiment. Different culture solution was respectively added for different groups. RPMI1640 culture solution was added for HMCs in the negative control group, while HMCs in the rest 3 groups were cultured by 100 nmol/L insulin for 24 h. Meanwhile, HMCs from the JTYSR group and the LY294002 group were exposed to 125 mg/L JTYSR and 80 micromol/L LY294002 respectively for further 48 h. The proliferation of HMCs was detected by MTT and flow cytometry. The protein expression of IRS-1 and PI-3K in HMC was detected by immunohistochemical assay and Western blot. Results The proliferation of HMCs induced by high insulin could be significantly lowered, and the protein expression of IRS-1 and PI-3K could be down-regulated in the JTYSR group and the LY294002 group (P <0.01). Compared with the LY294002 group, the protein expression of IRS-1 and PI-3K could be slightly down-regulated in the JTYSR group (P <0.05).</p><p><b>CONCLUSION</b>JTYSR could lower high insulin induced proliferation of HMCs, and its mechanism might be related to insulin signaling pathway.</p>


Assuntos
Humanos , Proliferação de Células , Cromonas , Medicamentos de Ervas Chinesas , Farmacologia , Proteínas Substratos do Receptor de Insulina , Metabolismo , Células Mesangiais , Fisiologia , Morfolinas , Fosfatidilinositol 3-Quinase , Metabolismo , Fosfatidilinositol 3-Quinases , Metabolismo , Transdução de Sinais
2.
China Journal of Chinese Materia Medica ; (24): 257-259, 2003.
Artigo em Chinês | WPRIM | ID: wpr-266772

RESUMO

<p><b>OBJECTIVE</b>To develop a new drug of treating the hepatitis C virus by studing the mechanisms of songzhi pills.</p><p><b>METHOD</b>The four-wee old mice and two-month old rats were chosen to induce interferon.</p><p><b>RESULT</b>The contents of interferon among the groups treated with songzhi pills were significantly higher than those of the normal group and the model group (P < 0.01),</p><p><b>CONCLUSIONS</b>Songzhi pills may have the function of inducing interferon.</p>


Assuntos
Animais , Feminino , Masculino , Camundongos , Ratos , Combinação de Medicamentos , Medicamentos de Ervas Chinesas , Farmacologia , Gardenia , Química , Interferon gama , Plantas Medicinais , Química , Polyporales , Química , Ratos Sprague-Dawley
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