Avaliaçöes ultra-sonográfica, macroscópica e histológica da biopsia testicular em ovinos / Assessment of ultrasonographic images and gross and microscopic lesions of the testicular biopsy in sheep

Devido ao fato da biopsia testicular poder acarretar hemorragia, inflamaçäo, degeneraçäo, aderência e fibrose, especialmente com as técnicas incisionais ou abertas, este trabalho avaliou a aplicabilidade de uma técnica menos invasiva (biopsia com agulha Tru-Cut) em ovinos na obtençäo de material para histologia e acompanhou as lesöes testiculares posteriores. Trinta carneiros foram igualmente divididos em três grupos: 1) controle, animais näo submetidos à biopsia; 2) submetidos à biopsia + cola de fibrina nos locais da biopsia testicular e incisöes da pele; e 3) submetidos à biopsia + sutura da pele escrotal após a biopsia. Exames ultra-sonográficos foram realizados pré e pós biopsia. No centésimodia, os testículos foram avaliados macro e microscopicamente após orquiectomia. A ultra-sonografia permitiu mapear as alteraçöes ocorridas e acompanhar a evoluçäo das seqüelas. Ao exame macroscópico, pequenas áreas de calcificaçäo foram observadas em 55 e 70 por cento dos testículos nos grupos 2 e 3, respectivamente. A biopsia com agulha Tru-Cut forneceu material suficiente para histologia mas ocasionou lesöes focais restritas à área biopsiada. Apesar da possível ocorrência de calcificaçäo e outras lesöes mínimas, foi demonstrado que a biopsia com agulha Tru-Cut em ovinos é um procedimento seguro por näo ter comprometido significativamente as características estruturais e funcionais dos testículos

Use of fibrin glue derived from snake venom in testicular biopsy of rams

Sequelae due to testicular biopsy such as hemorrhage, adhesion and fibrosis may be limiting factors to the use of this surgical procedure. Fibrin glue (FG) derived from snake venom was used to minimize these sequelae, as well as to evaluate its healing property in tunica vaginalis and scrotal skin or rams. Applicability of fibrin glue derived from snake venom was tested in different tissues of other animals such as in sciatic nerve and colon of rats and skin of rabbits. In the present study, 30 healthy adult rams were used. They were divided into 3 groups of 10 animals each as follows: G1: fibrin glue group (application of fibrin glue on puncutre sites and skin incisions after bilateral testicular biopsy with a Tru-Cut needle); G2: swab/nylon group (hemostasis by compression with a swab on puncture sites and skin suturing with nylon after biopsy) and G3: control group (the animals were not subjected either to biopsy or to surgery). On the 20th day after biopsy, the presence of adhesion strands between the sites of skin incision and testicle was evaluated by palpation. Adhecion strands were found in three testicles (15 per cent) in G1 and in two testicles (10 per cent) in G2. One hundred days after biopsy, orchiectomy was carried out and the material collected was assessed for subcutaneous (SC) and/or tunica vaginalis adhesions. G3 did not present any abnormality. Groups G1 and G2 presented four testicles each (20 per cent) with adhesion between the tunics and biopsy site. On the other hand, subcutaneous adhesions were found once (5 per cent) in G1 and three times (15 per cent) in G2. Fibrin glue showed to be of easy application, required short postoperative monitoring, presented fast and good-quality healing property and tended to reduce formation of subcutaneous adhesion.