RESUMO
No abstract available.
Assuntos
Humanos , Antibacterianos/farmacologia , Compostos Azabicíclicos/farmacologia , Proteínas de Bactérias/genética , Ceftazidima/farmacologia , Cefalosporinas/farmacologia , DNA Bacteriano/genética , Farmacorresistência Bacteriana/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Ácido Penicilânico/análogos & derivados , Pseudomonas aeruginosa/efeitos dos fármacos , Reação em Cadeia da Polimerase em Tempo RealRESUMO
Direct plating of synovial fluid (SF) on agar-based media often fails to identify pathogens in septic arthritis (SA). We developed a PCR assay for the simultaneous detection of Kingella kingae and Staphylococcus aureus from SF to evaluate molecular detection in SF and to estimate the incidence of K. kingae in SA in North America. The assay was based on detection of the cpn60 gene of K. kingae and the spa gene of S. aureus in multiplex real-time PCR. K. kingae was identified in 50% of patients between 0 and 5 yr of age (n=6) but not in any patients >18 yr old (n=105). Direct plating of SF on agar-based media failed to detect K. kingae in all samples. The PCR assay was inferior to the culture-based method for S. aureus, detecting only 50% of culture-positive cases. Our findings suggest that K. kingae is a common pathogen in pediatric SA in North America, in agreement with previous reports from Europe. PCR-based assays for the detection of K. kingae may be considered in children with SA, especially in those with a high degree of clinical suspicion.
Assuntos
Adulto , Criança , Pré-Escolar , Humanos , Lactente , Recém-Nascido , Artrite Infecciosa/diagnóstico , Proteínas de Bactérias/genética , DNA Bacteriano/análise , Kingella kingae/genética , Reação em Cadeia da Polimerase em Tempo Real , Staphylococcus aureus/genética , Líquido Sinovial/microbiologiaRESUMO
No abstract available.