RESUMO
Two types of inclusion bodies were consistently observed under light microscopy in bean (Phaseolus vulgaris) leaf tissue infected with Bean rugose mosaic virus (BRMV), a species of the genus Comovirus, family Comoviridae. One type consisted of vacuolated inclusions found mainly in the cytoplasm of epidermal cells. The other type consisted of abundant crystalloid inclusions of different sizes and shapes found consistently in glandular hairs, guard cells, phloem tissue, xylem elements and occasionally in epidermal and mesophyll tissues. The two types of inclusion bodies stained with Azure A and Luxol Brilliant Green Bl-Calcomine Orange 2RS (O-G), and were similar to those seen to be caused by other species of comoviruses.
Assuntos
Comovirus , Doenças das Plantas/virologia , Corpos de Inclusão Viral , Plantas MedicinaisRESUMO
Normal and pathological human nasal mucous biopsies were used and obtained surgically. Pathological one were taken from cocaine addicted patients who had been using this drug during many years mainly by snorting via. From the clinical point of view all patients presented similar symptoms among which the most common were acute and chronic rhinitis, sneeze and bleeding. All cases include showed evident tissue disorganization that ultrastructurally present partial or total lost of cillia as well as disruption between epithelia and basal lamina. Although, normal tissue was substituted by abundant fibrous tissue explaining why this tissue lost its normal function. All described alterations were analyzed by ransmission electron microscope and scanning electron microscope