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1.
Braz. j. med. biol. res ; 40(7): 985-992, July 2007. tab, graf
Artigo em Inglês | LILACS | ID: lil-455992

RESUMO

The objective of the present study was to investigate the effects of the direct addition of pentoxifylline (PF) to the ejaculates of men with poor sperm quality before freezing on post-thaw sperm motility, viability, acrosome integrity, and agonist-induced acrosome reaction. Semen specimens from 16 infertile men with impaired sperm count and motility (oligoasthenozoospermia) were divided into two equal aliquots: one received no treatment (control) while the other was incubated with 5 mM PF (treated). Both aliquots were cryopreserved by the liquid nitrogen vapor method. Motility was assessed according to WHO criteria. Acrosome integrity and spontaneous and calcium ionophore-induced acrosome reactions were assessed with fluorescein isothiocyanate-conjugated peanut agglutinin combined with a supra-vital dye (Hoechst-33258). Cryopreservation impaired sperm motility (percentage reduction: 87.4 (interquartile range, IQ: 70.3-92.9) vs 89.1 (IQ: 72.7-96.0 percent)), viability (25.9 (IQ: 22.2-29.7) vs 25.6 (IQ: 19.7-40.3 percent)) and acrosome integrity (18.9 (IQ: 5.4-38.9) vs 26.8 (IQ: 0.0-45.2 percent)) to the same extent in both treated and control aliquots. However, PF treatment before freezing improved the acrosome reaction to ionophore challenge test scores in cryopreserved spermatozoa (9.7 (IQ: 6.6-19.7) vs 4.8 (IQ: 0.5-6.8 percent); P = 0.002). These data show that pre-freeze treatment of poor quality human sperm with pentoxifylline did not improve post-thaw motility or viability nor did it prevent acrosomal loss during the freeze-thaw process. However, PF, as used, improved the ability of thawed spermatozoa to undergo the acrosome reaction in response to calcium ionophore. The present data indicate that treatment of poor quality human sperm with PF may enhance post-thaw sperm fertilizing ability.


Assuntos
Humanos , Masculino , Reação Acrossômica/efeitos dos fármacos , Pentoxifilina/farmacologia , Inibidores de Fosfodiesterase/farmacologia , Motilidade dos Espermatozoides/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Estudos de Casos e Controles , Criopreservação/métodos , Contagem de Espermatozoides , Preservação do Sêmen/métodos
2.
Braz. j. med. biol. res ; 36(6): 787-793, June 2003. ilus, tab
Artigo em Inglês | LILACS | ID: lil-340669

RESUMO

We determined the prevalence of Y chromosome deletions in a population of 60 Brazilian nonobstructive azoospermic and severely oligozoospermic men. PCR-based screening of microdeletions was performed on lymphocyte DNA for the presence of 14 sequence-tagged sites (STS) located in the azoospermic factor (AZF) on the Yq chromosome. All STS were amplified efficiently in samples from 12 fertile men tested, but failed to be amplified in samples from fertile women, indicating the specificity of PCR conditions for Yq screening. Overall, 4 of the 60 infertile patients tested (6.7 percent) exhibited deletion of the Y chromosome, 2 of them being severely oligozoospermic patients (P10 and P32) and 2 azoospermic men (patients P47 and P57). Patients P47 and P57 presented larger deletions in the AZFa, AZFb and AZFc subregions, with apparent loss of Yq material evidenced by karyotype analysis. Patients P10 and P32 presented deletions confined to the AZFc region, involving the DAZ locus. Male relatives of patients P10 and P32 had no Y chromosome deletions and presented a normal karyotype, suggesting a de novo status of the deletions found. Our data add to the growing literature showing that microdeletions of the Y chromosome can be the cause of male idiopathic infertility


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Deleção Cromossômica , Infertilidade Masculina , Cromossomo Y , Oligospermia , Reação em Cadeia da Polimerase , Prevalência , Sitios de Sequências Rotuladas , Índice de Gravidade de Doença
3.
Hansen. int ; 16(1/2): 35-43, dez. 1991. ilus, tab
Artigo em Inglês | LILACS | ID: lil-143819

RESUMO

Estudaram-se 58 fragmentos testiculares e o espermograma de 10 indivíduos normais e 24 hansenianos. Verificaram-se as alteraçöes gerais e precoces na histologia, a freqüência de espermátides jovens no lumem tubular e a medida dos diâmetros de 1.450 túbulos seminíferos. De acordo com os resultados obtidos, näo foi possível estabelecer uma correlaçäo qualitativa entre as descriçöes histológicas e ou quantitativa entre a morfometria, e um grupo clínico específico de hansenianos. Assim, também, a simultaneidade e a variabilidade dos achados observados em um mesmo testículo, se opöe às fases evolutivas, descritas por H. Grabstald & L.L. Swan e à classificaçäo proposta por B. Kumar et al. Os autores sugerem que as alteraçöes testiculares sejam conseqüência de uma auto-agressäo à glândula


Assuntos
Adolescente , Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Hanseníase/patologia , Testículo/patologia , Contagem de Espermatozoides , Ducto Deferente/patologia
4.
Rev. Hosp. Säo Paulo Esc. Paul. Med ; 2(3/4): 64-6, July-Dec. 1990. tab
Artigo em Inglês | LILACS | ID: lil-140654

RESUMO

In this investigation a comparison was made between the method for dounting leucocytes in first and final voided centrifuged urine samples and that for noncentrifuged urine samples. Among 417 patients, with a presumed diagnosis of urethritis, 216 had their diagnosis confirmed by the centrifuged urine method and 221 by that which did not use centrifugation. This difference was considered signficant and showed the superiority of the method which did not use centrifugation


Assuntos
Adolescente , Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Uretrite/diagnóstico , Centrifugação , Contagem de Leucócitos/métodos
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