RESUMO
Objective @#To investigate whether exogenous calcium-binding protein A8 / A9 ( S100A8 / A9) promotes the migration and invasion of oral squamous cell carcinoma ( OSCC) cells CAL-27 and SCC-25 through Wnt / β - catenin．@*Methods @#CAL-27 and SCC-25 were cultured with S100A8 and S100A9 proteins respectively or together， and the expression of β-catenin was detected by Western blot．20 μg / ml Wnt / β-catenin pathway inhibitor DKK-1 was added，the expression of β-catenin was detected by Western blot and qPCR ; Transwell assay was used to compare the cell invasion and migration ability of normal control group，S100A8 / A9 protein added group，DKK-1 add- ed group，and DKK-1 added and S100A8 / A9 protein recovery group ; Western blot was used to detect the expres- sion of c-Myc and cyclinD1 . @*Results @#S100A8 and S100A9 could activate the Wnt / β-catenin pathway．Compared with S100A8 and S100A9 cultured separately，the expression of β-catenin in CAL-27 and SCC-25 cells was up-reg- ulated with time when co-cultured，and the qPCR results were consistent with them ; in the immunofluorescence ex- periment，changes in the expression of β-catenin were observed in the cytoplasm and nucleus of SCC-25 and CAL- 27 cells ，and the expression of β-catenin protein in SCC-25 and CAL-27 cells increased at 48 h (P ＜0. 05 ) . Adding DKK-1，Western blot and qPCR showed that the expression of β-catenin decreased (P＜0. 001) ; the num- ber of cell migration and invasion in the S100A8 / A9 group was more than that in the blank group (P ＜0. 001) ， the number of cells invading and migrating decreased in the DKK-1 inhibitor group，and the number of cells in the DKK-1 and S100A8 / A9 protein recovery group decreased，suggesting that DKK-1 could inhibit the promoting effect of exogenous S100A8 / A9 protein on the invasion and migration ability of SCC-25 and CAL-27 cells，and the differ- ence was statistically significant (P＜0. 001) ; adding Wnt / β-catenin pathway inhibitor DKK-1，Western blot ex- periments showed that the expressions of key molecules c-Myc and cyclinD1 in the Wnt / β-catenin pathway de- creased (P＜0. 001) ．@*Conclusion @#Exogenous S100A8 / A9 can promote the invasion and migration of SCC-25 and CAL-27 cells by activating the Wnt / β-catenin signaling pathway.