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1.
Indian J Biochem Biophys ; 1989 Oct; 26(5): 293-5
Artigo em Inglês | IMSEAR | ID: sea-26640

RESUMO

A soluble extract capable of transcribing Sarcoma-180 chromatin and DNA in a cell-free transcription system was prepared from Sarcoma-180 mouse ascites tumour cells. Incorporation of [3H]UTP into trichloroacetic acid-precipitable fraction is (i) reduced by 50% on removing DNase I hypersensitive sites of chromatin and (ii) inhibited by DNA binding antitumour anthracyclines, suggesting that this cell-free assay represents true transcription of active genes of Sarcoma-180 chromatin. Preparation of this soluble extract from mouse ascites tumour cells thus presents a very convenient way of studying cell-free transcription of active genes of chromatin and effect of antitumour agents on chromatin transcription.


Assuntos
Animais , Antibióticos Antineoplásicos/farmacologia , Sistema Livre de Células , Cromatina/metabolismo , Camundongos , Sarcoma 180/genética , Transcrição Gênica/efeitos dos fármacos , Células Tumorais Cultivadas
2.
J Indian Med Assoc ; 1988 Mar; 86(3): 83-5
Artigo em Inglês | IMSEAR | ID: sea-104252
3.
7.
J Indian Med Assoc ; 1967 May; 48(9): 438-40
Artigo em Inglês | IMSEAR | ID: sea-96440
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