Effect of Delonix regia (Boj. Ex Hook.) Raf. stem bark extract against experimentally induced ulcers in rats.

Delonix regia, commonly called Flame Tree or Flamboyant (locally, Gul Mohor) is a common tree traditionally used to treat various diseases like gastric problems, body pain, rheumatic pains of joints and wound healing. Here, we carried out biological profiling of Delonix regia as antiulcer agent. Antiulcer activity of the ethanol extract from stem bark was evaluated on pylorus ligation and indomethacin induced ulcer in Wistar albino rats. Ethanol extract from stem bark of D.regia was administered at the doses 100, 200 and 400 mg/kg/day, p.o. for 7 days. Ulcer index, gastric pH, volume, free acidity, total acidity, total carbohydrate (TC), protein (P), mucin content (TC/P) and gastric mucus were evaluated in pylorus ligation model, while ulcer index, malondialdehyde, GSH, PGE2, and gastric mucus were estimated in the indomethacin induced ulcer model. Ex vivo assay for the activity of H+/K+-ATPase was also done. The results showed significant inhibition on H+/K+-ATPase in a dose dependent manner and comparableto their respective positive control group of rats demonstrating that ethanol extract of stem bark of Delonix regia possesses significant antiulcer properties.

Development and validation of selective UV spectrophotometric analytical method for budesonide pure sample.

Budesonide is a very potent corticosteroid, used for bronchial asthma and inflammatory bowel disease. Objective of the present investigation is to develop the simple and selective UV spectrophotometric method for quantification of budesonide in bulk sample. Absorption maximum of budesonide was found to be 246.0 nm and obeyed the beers law in the concentration range of 1.4 to 25 μ g/ml. Calibration curve shows a linear relationship between the absorbance and concentration in the range of 2 to 10 μ g/ml and the limit of detection is 0.01 μ g/ml. The limit of quantification was found to be 1.4 μg/ml. The method was validated for repeatability, accuracy and precision. The percent amount of recovery was 99 - 100% with minimum standard deviation less than 1%. Obtained results showed there is minimum intra day and inter day variation. The excipients present in the preparation did not interfered during the analysis. Developed analytical UV spectrophotometric method is simple, rapid and reproducible and further it can be used for estimation of drug in bulk and colon matrix tablet dosage form.

Rapid analytical procedure for Citicoline in bulk and pharmaceutical dosage form by UV Spectrophotometer.

A simple, rapid, accurate, precise, and inexpensive method for the determination of citicoline has been developed using double beam UV spectrophotometer. Ultraviolet spectrophotometric analysis was carried out on a Shimadzu UV 1800 (Shimadzu, Japan) spectrophotometer, in a 1cm quartz cuvette. Citicoline has absorption maxima at 272 nm and the measurements were obtained against distilled water. Beer Lambert’s law was obeyed in the concentration range of 5-50μg/ml with correlation coefficient (r2) 0.9998. The analytical method was successfully validated in order to verify its proper selectivity, linearity, accuracy and precision for the goal intended and its further implementation for the quantification of the active compound in the pharmaceutical speciality for quality control.

Evaluation of Antidiarrhoeal activity of extract from roots of Rumex hastatus (Family: Polygonaceae) on experimental animals.

Rumex hastatus is fairly common small shrub, growing on dry slopes, rocks and walls between 700-2500 m, typically in North Indian hill station. Roots of Rumex hastatus have been used in Ayurveda and other folk medicines for the treatment of diarrhoea. To justify its folklore, present study was undertaken to investigate the antidiarrhoeal activity of the ethanolic extract from the roots of Rumex hastatus (EERH). Preliminary phytochemical screening, acute toxicity study and antidiarrhoeal activity of were studied on castor oil induced diarrhoea and normal gastrointestinal models of rats at 100, 150 and 200mg/kg body weight. The preliminary phytochemical screening of the ethanolic extract from the roots of Rumex hastatus (EERH) results with the presence of anthraquinone glycoside, tannins, flavonoids etc., LD50> 2000mg/kg. The doses of EERH significantly decreased (P<0.001) the total number of diarrhoeal faeces. PI decreases doses dependentally (100, 150 and 200mg/kg orally) of EERH and was comparable with standard drug atropine sulphate. Conclusively, EERH has the antidiarrhoeal activity in experimental rats.