Screening, preparation and biological activity of human monoclonal antibodies against postsynaptic short-chain neurotoxins fromLapemis curtus / 解放军医学杂志

Objective To prepare human anti-postsynaptic neurotoxin monoclonal antibody from phage antibody library using recombined postsynaptic short-chain neurotoxins ofLapemis curtus.Methods The three postsynaptic neurotoxins were expressed inEscherichia coli and phage antibodies against neurotoxins were screened. The obtained scFvs were further constructed to full antibodies. The antigen binding ability, biochemical and pharmacokinetic characteristics of the depurated antibodies were evaluated, and the anti-toxin effects of the antibody drugs were verifiedin vivo.Results Two positive scFvs with specific binding ability to all the three neurotoxins were obtained after 4 rounds of panning, and then the full antibodies were generated, expressed and purified. Antibody binding specificity was further confirmed. Pharmacokinetics of these two antibodies, SM-SD-911 and SM-SD-861 were similar to a conventional IgG molecule. SM-SD-911 and SM-SD-861 also showed strong antitoxin effectin vivo. Conclusionfull human anti-postsynaptic neurotoxin antibody has been successfully obtained by using recombinant neurotoxin technology and a large phage antibody library which may achieve clinical efficacy in navy medical applications.

Research on synchronous control by remote apparatus based on Windows API / 医疗卫生装备

This paper gives the techniques and steps of creating remote control instruments system using Windows API functions,and explains its hardware and software structures.The whole system is proved to be stable and reliable by experiment.It can provide a new experimental method for biomedical research,which enable the construction of multifunctional experiment platform.

Pseudo-color enhancement in calcium sparks detection of skeletal muscle fiber / 医疗卫生装备

Fluorescence probe Fluo-3AM can be luminary in confocal microscope after combining Ca2+,So nomadic Ca2+ in cytoplasm can be measured and tested by this way.Some confocal laser scan images are obtained after calcium in muscle fibers signed with F1uo-3AM. Then mapping function is used to convert the gray level image into colorful image by pseudo-color enhancement theory in digital image.Compared to the original gray level image,the processed pseudo-color image could be more easily distinguished.So the methods had a great value in Ca2+ detection fields.

Signal noise filtering of patch clamp based on Wavelet transform / 医疗卫生装备

Wavelet transform(WT) is a powerful technique in signal separation and can improve the signal-to-noise ratio by separating the white noise and useful signal.The method in the paper introduces the concepts of Wavelet transform.According to the analysis of Wavelet coefficients between the noise and signal at different levels,the appropriate filter is realized.The patch clamp experiment results demonstrate the feasibility of the method.

Method of calling MATLAB in VB application based on ActiveX / 医疗卫生装备

Objective To discuss the method of calling MATLAB in VB application program based on ActiveX.Methods MATLAB provided automatic service support to ActiveX,and MATLAB could be called as ActiveX of interpreter through ActiveX automatic interface.Results Good effect was achieved when this method was applied to noise filtering of patch clamp.Conclusion By using this method,users can embedded mix programming in self-developed application software conveniently,thus reducing the difficulties in programming.

Development of a detection system of the enzyme-linked analyzer / 医疗卫生装备

In allusion to high labor intensity, low working efficiency and high error rate in hospital laboratory, a detection system of the enzyme-linked analyzer based on Multiscan EX instrument is developed. Being easy to operate, the detection system proves effective in enhancing working efficiency. It deserves popularizing because of its comprehensive applications to the detection.

Development of microcomputer system for SCCA marker detection / 医疗卫生装备

With a PII-300 computer, a microcomputer system for SCCA marker detection is developed in this paper, which is based on Windows operating system and Visual Basic 6.0. More than 1,000 cases have proved that the results from the system completely accord with the one from artificial detection. With this system, such disadvantages are conquered as high labor intensity, low working efficiency and high error rate, and a quantitative detection method for SCCA marker comes into existence.

The development of an intelligentized microcomputer system for HBV marker detection and reporting / 医疗卫生装备

With a PII-300 computer, an intelligentized microcomputer system for HBV marker detection and reporting is developed in this paper, which is based on Windows operating system and accomplished through Visual Basic6.0. More than 10, 000 cases have proved that the result from the system accords with the one from artificial detection completely. This system solves the problems in hospital laboratory such as high labor intensity, low working efficiency and high error rate, and thus a quantitative detection method for HBV marker comes into existence.

Nonlinear detection method of ECG using wavelet transform / 医疗卫生装备

The nonlinear wavelet method is used to detect ECG signal. The ECG signal disrupted by high-frequency noise is decomposed with a dyadic Daubechies wavelet according to Mallat algorithm. The principle of the nonlinear wavelet detection method is discussed. Along with the Lipschitz criterion, it is applied to denoising the disrupted ECG signal. The ECG signal is effectively recovered while denoised.

Querying and Searching of XML Electronic Medical Records / 医疗卫生装备

Objective To implement XML-based electronic medical records' searching and querying using new technology of XML storing and indexing.Methods The information in electronic records was expressed with CDA XML standard,and XML Query and XML IR were used for querying of electronic records.Results Based on XML querying and indexing models,a new electronic medical record XML querying and searching system was built.Conclusion Querying and searching over XML database has been paid more attention to,with wide use of data adapting to HL7 CDA XML standard in electronic medical records.

Application Study of Wavelet Transform in Calcium Sparks Image De-noising / 医疗卫生装备

Objective To study on wavelet transform in calcium sparks image de-noising when excitation contraction cou-pling(ECC) was studied and researched by scanning confocal microscopy,in order to elucidate the mechanism of ECC.But due to the noise exist in most calcium sparks image,it is hardly to recognize and analyze the useful information of calcium sparks.So how to increase the signal-to-noise rate is very important in calcium spark studying.Methods By using the self-developed software based on multi-scale wavelet transform to remove the noise exists in the calcium sparks images.Results The experiments shows that the method can be increased the signal-to-noise rate in about 80 calcium sparks images.Con-clusion The new method for calcium spark expression and spatial and temporal analysis is provided.