RESUMO
The detection technology based on micro-nanofluidics has been developed fast with wide applications in biochemical analysis and clinical diagnostics because of its small sample volume requirement, rapid detection and portability. Recently, nanofluidics emerges for DNA sequencing and biomarker detection. With nanopore technology,whole genome sequence in 24 hours with a cost of lower than $1000 could be realized. However, improvement in the detection accuracy and repeatability are still desired for clinic diagnostics. Nanopore technology could be powerful tools for clinical diagnostics, providing new opportunities for laboratory medicine.
RESUMO
The detection technology based on micro-nanofluidics has been developed fast with wide applications in biochemical analysis and clinical diagnostics because of its small sample volume requirement, rapid detection and portability. Recently, nanofluidics emerges for DNA sequencing and biomarker detection. With nanopore technology,whole genome sequence in 24 hours with a cost of lower than$1000 could be realized. However, improvement in the detection accuracy and repeatability are still desired for clinic diagnostics. Nanopore technology could be powerful tools for clinical diagnostics, providing new opportunities for laboratory medicine.
RESUMO
Objective To study the expression and significance of CD68 and TGF-β2 in benign prostatic hyperplasia(BPH).Methods The immunohistochemistry PV two step method was used to detect the expression level of CD68 and TGF-β2 in 90 cases of benign prostatic hyperplasia and combined with clinical data were analyzed.Results Positive rate of CD68 as macrophages marker was 86%(77/90) in the epithelial cells,positive rate of TGF-β2 as transforming growth factor marker was 79%(71/90) in epithelial cells and stromal cells. Immune inlfammation mediated by macrophages,with coloring degree deepening,the degree of immune inlfammation increased,larger prostate volume(P<0.01),IPSS score higher (P<0.01),maximum urinary lfow rate lower(P<0.01),the dffierences between groups were statistically significant.Conclusions This point may indicate immune inlfammation play an important role in the development process of BPH and help to complete the pathogenisis theory.